地塞米松抑制哮喘大鼠肺组织 p38 蛋白激酶的表达

中国临床药理学与治疗学 ›› 2005, Vol. 10 ›› Issue (5): 555-558.

地塞米松抑制哮喘大鼠肺组织 p38 蛋白激酶的表达

黄翠萍, 杨和平, 张珍祥¹, 徐永健¹

咸宁学院医学院内科, 咸宁 437100, 湖北;
¹华中科技大学同济医学院附属同济医院呼吸内科, 武汉 430030, 湖北

收稿日期:2005-01-14 修回日期:2005-03-14 出版日期:2005-05-26 发布日期:2020-11-19
通讯作者: 黄翠萍,女, 医学博士,副教授, 研究方向:哮喘、急性肺损伤。Tel:0715-8268279 E-mail:huangcuiping @hotmail.com
基金资助:
本课题为湖北省教育厅科研基金资助项目(No2003B004)

Changes of p38 mitogen-activated protein kinase in lung tissue and effects of dexamethasone in asthmatic rats

HUANG Cui-ping, YANG He-ping, ZHANG Zhen-xiang¹, XU Yong-jian¹

Department of Medicine, Affiliated Hospital of Xianning Medical College, Xianning 437100, Hubei, China;
¹Depart-ment of Respiratory Medicine, Tongji Hospital, Tongji Medicial College, HuazhongUniversity of Science and Technology,Wuhan430030, Hubei, China

Received:2005-01-14 Revised:2005-03-14 Online:2005-05-26 Published:2020-11-19

摘要/Abstract

摘要： 目的: 探讨支气管哮喘大鼠肺组织 p38 蛋白激酶(p38 MAPK) 表达的变化以及地塞米松对其影响。方法: 复制大鼠哮喘模型, 随机分成 3 组 :正常对照组、哮喘对照组和地塞米松(DEX) 干预组。分别采用酶联免疫吸附法(ELISA) 和蛋白质印迹检测支气管肺泡灌洗液(BALF) IL-5 含量和肺组织磷酸化p38 MAPK 表达的变化, 并观察气道阻力、BALF中 EOS 计数以及肺组织病理学变化。结果: 哮喘对照组大鼠肺组织磷酸化 p38 MAPK 表达水平及气道阻力、BALF 中 IL-5 含量和 EOS 计数均较正常对照组显著增加(P<0.01);DEX 干预组上述指标较哮喘对照组显著降低(P<0.01), 肺组织病理学损伤程度明显减轻。肺组织磷酸化 p38 MAPK 表达水平与气道阻力、BALF 中 IL-5 含量和 EOS 计数之间分别呈显著正相关(r =0.77、0.63、0.65, P<0.01)。结论: p38 MAPK 可能参与了支气管哮喘的发病过程。DEX 对哮喘的治疗作用至少部分与抑制磷酸化 p38 MAPK 的表达有关。

关键词: 支气管哮喘, 丝裂原活化蛋白激酶, 地塞米松, 白细胞介素

Abstract: AIM: To identify the changes of p38 mi-togen-activated protein kinase (p38MAPK) in lung tissue of asthmatic rats and the effects of dexamethasone on it. METHODS: Ovalbumin (OVA) was injected intraper-itoneally and inhaled to produce the asthmatic model. Twenty ratswere randomly divided into three groups:con-trol group, asthma group and DEX group. The concentra-tion of IL-5 in BALF and the expression of phospho-p38 MAPK in lung tissue were measured by ELISA and West-ern blot, respectively. RESULTS: The expression of phospho-p38 MAPK was up-regulated in the lung of asth-matic rats.The injection of DEX intraperitoneally de-creased the expression of phospho-p38 MAPK and abol-ished the increases of airway resistance, the concentration of IL-5 and the number of eosinophil in BALF. His-topathologic damage of lung tissue was alleviated.There were positive correlations between the expression of phos-pho-p38 MAPK and airway resistance, the concentration of IL-5 and the number of eosinophil in BALF. CONCLUSION: p38 MAPK may play a role in pathological process of asthma. DEX can effectively treat asthma by inhibiting the expression of p38 MAPK.

Key words: asthma, p38 mitogen-activated protein kinase, dexamethasone, interleukine

中图分类号:

R966

黄翠萍, 杨和平, 张珍祥, 徐永健. 地塞米松抑制哮喘大鼠肺组织 p38 蛋白激酶的表达[J]. 中国临床药理学与治疗学, 2005, 10(5): 555-558.

HUANG Cui-ping, YANG He-ping, ZHANG Zhen-xiang, XU Yong-jian. Changes of p38 mitogen-activated protein kinase in lung tissue and effects of dexamethasone in asthmatic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(5): 555-558.

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