心理应激对溃疡性结肠炎病情及疗效的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (1): 86-90.

心理应激对溃疡性结肠炎病情及疗效的影响

王昊, 吴万春, 韩真, 金道友, 汪萌芽¹

皖南医学院弋矶山医院消化内科, ¹生理教研室, 芜湖 241000, 安徽

收稿日期:2005-12-20 修回日期:2005-12-30 出版日期:2006-01-06 发布日期:2020-10-23
通讯作者: 吴万春, 通讯作者, 男, 主任医师, 硕士生导师, 研究方向:胃肠动力。
作者简介:王昊, 男, 硕士研究生, 研究方向:心理应激和胃肠疾病。 Tel:13515535006 E-mail:howard_123@163.com 吴万春, 通讯作者, 男, 主任医师, 硕士生导师, 研究方向:胃肠动力。

Effects of psychological stress on pathogenetic condition and rapeutic effect of ulcerative colitis

WANG Hao, WU Wan-chun, HAN Zhen, JIN Dao-you, WANG Meng-ya

Department of Gastroenterology, Wannan Medical College, Wuhu 241001, Anhui, China

Received:2005-12-20 Revised:2005-12-30 Online:2006-01-06 Published:2020-10-23

摘要/Abstract

摘要： 目的 :通过观察心理应激对溃疡性结肠炎大鼠产生的影响并探讨其影响的机制 ;同时观察抗焦虑药物对处于应激状态下溃疡性结肠炎大鼠症状的干预作用及其作用机制。方法:80 只 Wistar 大鼠随机分为 UC 对照组、UC 应激组、UC +氯硝西泮组和 UC 应激 +氯硝西泮组各 20 只, 用免疫接种法建立溃疡性结肠炎大鼠模型后, 用猫恐吓的方法建立心理应激模型。建立心理应激模型期间分别给予各组生理盐水、氯硝西泮( 2 mg·kg ^-1 ) 灌胃。15 d 后处死大鼠, 以组织损伤评分、结肠组织 MPO 和 NO 活性等指标来评价其炎症和损伤程度。结肠组织损伤评分通过显微镜观察得出。并用分光光度分析法测定结肠组织MPO 、NO 活性, 放射免疫分析法( RIA) 检测大鼠血清中皮质醇的含量。结果 :应激模型组的血清皮质醇、肠组织 MPO 、NO 含量及结肠组织损伤评分较UC 对照组明显增高 ;UC 应激 +氯硝西泮组上述指标明显低于 UC 应激组 ;而 UC +氯硝西泮组上述指标与 UC 对照组无明显区别。结论 :心理应激可以明显加重溃疡性结肠炎的症状。苯二氮卓类药物本身无抗炎作用, 但可通过影响边缘系统和网状结构, 减轻了心理应激对内脏的影响, 缓解炎性肠病症状的加重。

关键词: 心理应激, 溃疡性结肠炎 , 氯硝西泮 , 大鼠

Abstract: AIM:To investigate the effects of psy- chological stress on ulcerative colitis rats and to explore the mechanisms of this ;meanwhile to investigate the ef- fects of anxiolytic drug on symptoms of ulcerative colitis rats under psychological stress and the mechanisms of it . METHODS:eighty male Wistar rats were randomly di- vided into four groups ( n =20) :UC control group ;UC stress group ;UC +clonazepam group ;UC stess +clonaz- epam group .After UC model was established with immu- nization, these rats were fightened by cat to set up psy- chological stress model .During establishing stress model, each groups were respectively received physiologic saline or clonazepam by intragastric administration .After 15d treatment, all rats were sacrified simultaneously .Colon mucosal inflammationand damage were assessed by mea- suring colon mass, morphologic damage score, colonic MPO and NO activity levels .Morphologic damage score was examined under microscope.Colonic MPO and NO was measured by spectrophotometric method .Serum hydrocortisone was determined by radioimmunoassay ( RIA) .RESULTS :Morphologic damage score, colonic MPO and NO, serum hydrocortisone of UC stress group were significantly increased compared with those of UC control group ;morphologic damage score, colonic MPO and NO, serum hydrocortisone of UC +clonazepam group were decreased markedly vs UC stress group ;Little differ- ence was observed between UC +clonazepam group and UC control group .CONCLUSION:Psychological stress can aggravate symptoms of UC.Although benzodiazepine have no anti-inflammatory action, it relieve effects on in- ternal organs of psychological stress and aggravation of symptoms of UC by to effect limbic system and formatio reticularis .

Key words: psychological stress ;ulcerative colitis , clonazepam ;rat

中图分类号:

R965 .1

王昊, 吴万春, 韩真, 金道友, 汪萌芽. 心理应激对溃疡性结肠炎病情及疗效的影响[J]. 中国临床药理学与治疗学, 2006, 11(1): 86-90.

WANG Hao, WU Wan-chun, HAN Zhen, JIN Dao-you, WANG Meng-ya. Effects of psychological stress on pathogenetic condition and rapeutic effect of ulcerative colitis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(1): 86-90.

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