辛伐他汀对高脂血症兔主动脉内皮细胞非神经性M受体的影响

中国临床药理学与治疗学 ›› 2006, Vol. 11 ›› Issue (11): 1270-1274.

辛伐他汀对高脂血症兔主动脉内皮细胞非神经性M受体的影响

李军, 王倩¹, 王汝欢¹, 龙超良, 汪海

军事医学科学院毒物药物研究所,北京 100850;
¹北京恩华医药研究院,北京 100039

收稿日期:2006-04-27 修回日期:2006-06-30 出版日期:2006-11-26 发布日期:2020-11-06
通讯作者: 汪海, 男, 博士, 研究员, 博士生导师, 研究方向:分子药理学和心脑血管新药。Tel:010-66932651 E-mail:wh@nic.bmi.ac.cn
作者简介:李军, 男, 博士研究生, 研究方向:分子药理学。Tel:010-66931557 　E-mail:lijunbmi@sina.com

Effect of simvastatin on non-neuronal M cholinoceptor of rabbit aorta endothelium with hyperlipemia

LI Jun, WANG Qian, WANG Ru-huan, LONG Chao-liang, WANG Hai

Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China

Received:2006-04-27 Revised:2006-06-30 Online:2006-11-26 Published:2020-11-06

摘要/Abstract

摘要： 目的探索辛伐他汀降脂治疗对介导内皮依赖性血管舒张的内皮细胞非神经性M₃ 受体基因表达的影响。方法高脂饮食饲喂诱发兔高脂血症模型, 同时设置辛伐他汀每日5 mg·kg^-1降脂干预,15周后, 取兔血浆测定血脂指标, 取主动脉环测定内皮依赖性和非内皮依赖性血管舒张活性, 并取主动脉内皮细胞以RT-PCR 方法检测M₃ 亚型的表达。结果高脂饲喂15 周造成高脂血症兔模型, 同时应用辛伐他汀干预可有效地降低血脂水平, 提高血管的内皮依赖性舒张活性, 提高介导内皮依赖性舒张活性的内皮非神经性M₃ 基因的表达。结论辛伐他汀可以逆转高脂血症状态下内皮非神经性M₃ 受体的表达, 改善血管的舒张活性。

关键词: 非神经性M 受体, 高脂血症, 内皮, 血管舒张, 辛伐他汀

Abstract: AIM: To investigate the effect of simvastatin on non-neuronal M3 mRNA expression of rabbit aorta with hyperlipemia.METHODS: Hype rlipemia rabbit model was induced by high lipid diet.The simvastatin grouPwas treated with 5 mg·kg^-1 everyday for 15 weeks.Endothelium dependent relaxation responses to acetylcholine( ACh) and non-endothelium dependent relaxation responses to nitroprusside ( SNP) of thoracic aortic rings were measured respectively.The M₃ mRNA expression of aorta endothelium were tested by RT-PCR.RESULTS: Compared with the hyperlipemia group, simvastatin treatment significantly degraded the plasma lipid concentration,attenuated the inhibition of endothelium dependent relaxation induced by hyperlipemia and improved the mRNA expression of M₃ in aorta endothelium.CONCLUSION: Simvastatin can reverse M₃ mRNA expression of aorta endothelium reduced by hyperlipemia and improve the vasodilatory activity.

Key words: non-neuronal M cholinoceptor, hyperlipemia, endothelium, vasodilation, simvastatin

中图分类号:

R965.2
R966

李军, 王倩, 王汝欢, 龙超良, 汪海. 辛伐他汀对高脂血症兔主动脉内皮细胞非神经性M受体的影响[J]. 中国临床药理学与治疗学, 2006, 11(11): 1270-1274.

LI Jun, WANG Qian, WANG Ru-huan, LONG Chao-liang, WANG Hai. Effect of simvastatin on non-neuronal M cholinoceptor of rabbit aorta endothelium with hyperlipemia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2006, 11(11): 1270-1274.

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