II、 III 组亲代谢型谷氨酸受体激动剂对脂多糖抑制C6 胶质瘤细胞摄取谷氨酸的影响

中国临床药理学与治疗学 ›› 2007, Vol. 12 ›› Issue (6): 626-629.

II、 III 组亲代谢型谷氨酸受体激动剂对脂多糖抑制C6 胶质瘤细胞摄取谷氨酸的影响

邹静, 姚红红, 胡刚

南京医科大学药理学系, 南京210029, 江苏

收稿日期:2007-04-24 修回日期:2007-05-21 发布日期:2020-11-09
通讯作者: 胡刚, 男, 博士, 教授, 博士生导师, 研究方向:神经退行性疾病的病因学与实验治疗学研究。Tel:025-86863108 E-mail:ghu @njmu. edu. cn
作者简介:邹静, 女, 硕士, 研究方向:神经退行性疾病的病因学与实验治疗学研究。Tel:025-86863169 E-mail:Lily-zou8 @hotmail. com
基金资助:
国家自然科学基金项目(30625038; 30600758; 30572172); 江苏省教育厅自然科学基金项目(05KJA31014; 06KJA31029); 江苏省卫生厅自然科学基金项目(K200501)

Agonists of group II and III metabotropic glutamate receptors reverse LPS-induced glutamate uptake inhibition in C6 glioma cells

ZOU Jing, YAO Hong-Hong, HU Gang

Department of Pharmacology, Nanjing Medical University, Nanjing 210029, Jiangsu, China

Received:2007-04-24 Revised:2007-05-21 Published:2020-11-09

摘要/Abstract

摘要： 目的:探讨II、 III 组亲代谢型谷氨酸受体(metabotropic glutamate receptors, mGluRs) 激动剂对脂多糖(LPS) 抑制C6 胶质瘤细胞摄取谷氨酸(glutamate,Glu) 的影响。方法:应用同位素标记法测定C6 胶质瘤细胞对[³H]-D, L-Glu 的摄取。应用Hoechst染色法、噻唑蓝比色法(MTT) 分别检测C6 胶质瘤细胞的凋亡、细胞活力。结果:LPS(4、 6 μg/mL) 显著抑制C6 胶质瘤细胞摄取[³H]-D, L-Glu, 抑制率分别达17. 6% 和22. 2% 。II 组mGluRs 激动剂DCGIV100 μmol/L 和III 组mGluRs 激动剂L-AP4100 μmol/L 逆转LPS 对C6 胶质瘤细胞摄取[³H]-D,L-Glu 的抑制作用, 这种逆转作用分别被II、 III 组mGluRs 拮抗剂APICA 和MSOP 取消。结论:DCG-IV和L-AP4 逆转LPS 引起的C6 胶质瘤细胞Glu 摄取抑制, 提示II、 III 组mGluRs 激动剂通过促进Glu 摄取, 降低细胞外Glu 浓度, 从而发挥神经保护作用。

关键词: 亲代谢型谷氨酸受体, 脂多糖, C6 胶质瘤细胞, 谷氨酸摄取

Abstract: AIM:To study whether agonists of group II and III metabotropic glutamate receptors (mGluRs) exert effects on LPS-induced glutamate uptake inhibition in C6 glioma cells.METHODS:The glutamate uptake into C6 glioma cells was measured by uptake of [³H]-D, Lglutamate; and the apoptosis and the viability of C6 glioma cells were investigated by Hoechst33342 and MTT methods, respectively.RESULTS:LPS (4, 6 μg/mL) inhibited glutamate uptake significantly compared with that in the control group without effect on the apoptosis and viability of C6 glioma cells. Pretreatment of C6 glioma cells with group II and III mGluRs agonists DCG-IV (100 μmol/L) and L-AP4(100 μmol/L) reversed LPS-induced glutamate uptake inhibition. These recovery effects were abolished by their respective antagonists APICA and MSOP.CONCLUSION:Activation of group II and III mGluRs recovers LPS-induced glutamate uptake inhibition in C6 glioma cells, suggesting the enhancement of glutamate uptake is involved in neuroprotective roles exerted by group II and III mGluRs agonists.

Key words: metabotropic glutamate receptors, lipopolysaccharide, C6 glioma cells, glutamate uptake

中图分类号:

R965. 2

邹静, 姚红红, 胡刚. II、 III 组亲代谢型谷氨酸受体激动剂对脂多糖抑制C6 胶质瘤细胞摄取谷氨酸的影响[J]. 中国临床药理学与治疗学, 2007, 12(6): 626-629.

ZOU Jing, YAO Hong-Hong, HU Gang. Agonists of group II and III metabotropic glutamate receptors reverse LPS-induced glutamate uptake inhibition in C6 glioma cells[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2007, 12(6): 626-629.

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