阿仑膦酸钠对骨-螺钉界面骨吸收的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (1): 74-78.

阿仑膦酸钠对骨-螺钉界面骨吸收的影响

郭艾, 陈亮

首都医科大学附属北京友谊医院骨科, 北京 100050

收稿日期:2007-04-12 修回日期:2008-01-19 出版日期:2008-01-26 发布日期:2020-10-13
作者简介:郭艾, 男, 硕士, 主任医师、副教授, 硕导, 研究方向:关节外科。Tel:010-63138353 　E-mail: guoaij@yahoo.com.cn

Impact of alendronate on bone resorption at the bone-screw interface

GUO Ai, CHEN Liang

Department of Orthopaedic Surgery, Capital Medical University, Beijing 100050, China

Received:2007-04-12 Revised:2008-01-19 Online:2008-01-26 Published:2020-10-13

摘要/Abstract

摘要： 目的从影像学和组织学方面评价阿仑膦酸钠对正常及骨质疏松大鼠骨-螺钉界面骨吸收的影响。方法取平均体重为332 g(287 ~ 351 g)成年Wister 雌鼠32 只, 随机分为4 组, C 组和D组行去势手术。8 周后各组于左侧后肢胫骨近端1 3 处置入皮质螺钉, 术后分别给予阿仑膦酸钠(A 组和C 组) 及生理盐水(B 组和D 组) 5 周, 安乐处死。通过影像学观察、组织涂片、抗酒石酸酸性磷酸酶(TRAP) 染色, 对其骨-螺钉界面的纤维吸收膜宽度与TRAP(+) 破骨细胞数量进行统计学分析。结果影像上可见, 术后当时, 螺钉周围并没有明显的溶骨区域。术后5 周时, 生理盐水组螺钉钉孔处相对阿仑膦酸钠组会呈现比较明显的宽低密度区;组织学方面, 阿仑膦酸钠组的纤维吸收膜膜宽及TRAP 染色阳性细胞数量均比生理盐水对照组明显减少, 差异有统计学意义。结论大鼠实验中, 无论是去势还是未去势, 阿仑膦酸钠均有效抑制了螺钉周围的骨溶解及纤维吸收膜的形成。

关键词: 大鼠, 阿仑膦酸钠, 纤维吸收膜, 抗酒石酸酸性磷酸酶

Abstract: AIM: To investigate the impact of alendronate on bone resorption of normal and ovariectomized rats at the bone-screw interface through radiographic and histologic findings. METHODS: Thirtytwo female Wister rats with a mean body weight of 332 g (287-351 g) were divided into four groups at random. Rats in group C and D were ovariectomized. 8 weeks later, the proximal one-third of the left tibia of all rats were inserted into self-drilling mini cortical screws. After operations, alendronate were used in group A and C and saline were given in group B and D. The rats were euthanized at 5 weeks after screws having been attached. Radiographic and histologic findings subsequently were analyzed. RESULTS: Radiographs confirmed that no osteolytic area was present around screws immediately after insertion, whereas 5 weeks after insertion, a wide and low-density area corresponding to the screw hole was evident in the saline groups compared with the alendronate groups. On histologic observation, the width of the fiber membrane and the number of TRAP-positive cells were decreased in the alendronate groups than those in the saline groups, and the difference was statistically significant. CONCLUSION: Alendronate effectly inhibits bone resorption of either normal or ovariectomized rats at the bone-screw interface in rats.

Key words: rats, alendronate, fiber membrane for absorption, tartrate-resistant acid phosphatase

中图分类号:

R965.2

郭艾, 陈亮. 阿仑膦酸钠对骨-螺钉界面骨吸收的影响[J]. 中国临床药理学与治疗学, 2008, 13(1): 74-78.

GUO Ai, CHEN Liang. Impact of alendronate on bone resorption at the bone-screw interface[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(1): 74-78.

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