参麦注射液对肺缺血-再灌注损伤时Fas/FasL 表达的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (12): 1355-1359.

参麦注射液对肺缺血-再灌注损伤时Fas/FasL 表达的影响

王俊¹, 林丽娜¹, 王万铁², 金立达¹, 邱晓晓², 戴雍月², 王青²

¹温州医学院附属第一医院麻醉科,
²温州医学院病理生理学教研室, 温州 325000, 浙江

收稿日期:2008-09-03 修回日期:2008-12-07 发布日期:2020-10-30
作者简介:王俊,男,硕士研究生,研究方向:器官保护。Tel:13456043482 E-mail:wangjun3778@126.com
基金资助:
浙江省中医药管理局(2008CA071)

Effects of Shenmai injection on the expression of Fas/FasL mRNA in pulmonary injury induced by ischemia-reperfusion in rabbits

WANG Jun¹, LIN Li-na¹, WANG Wan-tie², JIN Li-da¹, QIU Xiao-xiao², DAI Yong-yue², WANG Qin²

¹Department of Anesthesiology, the First Affiliated Hospital of Wenzhou Medical College;
²Department of Morbid Physiology, Wenzhou Medical College, Wenzhou 325000, Zhejiang, China

Received:2008-09-03 Revised:2008-12-07 Published:2020-10-30

摘要/Abstract

摘要： 目的探讨参麦注射液对肺缺血-再灌注损伤(PIRI) 时Fas/FasL 配体(Fas/FasL) 表达的影响。方法采用在体兔单肺原位缺血-再灌注模型。实验兔30 只, 随机分为假手术对照组(sham,n=10) 、肺缺血-再灌注组(I-R, n=10) 和肺缺血-再灌注加参麦注射液组(SM, n=10)。分别于再灌注3 h 取左肺组织, 观察Fas/FasL mRNA 定位表达、凋亡指数(AI) 、肺组织湿干重比(W D) 、肺损伤组织学定量评价指标(IQA) 及光镜、电镜下的组织形态学改变。结果 SM 组Fas/FasL mRNA在肺小动脉内(外) 膜、肺小静脉内膜、肺泡上皮及肺支气管上皮呈弱阳性表达, 明显低于I-R 组(P<0.05);AI 、W D 和IQA 值显著低于I-R 组(P <0.01 和P <0.05);肺组织形态学异常改变程度减轻。结论参麦注射液可下调肺组织Fas/FasLmRNA 的表达而减轻细胞凋亡, 对PIRI 发挥积极的防治作用。

关键词: 再灌注损伤, 肺, Fas/Fas 配体, 细胞凋亡, 参麦注射液

Abstract: AIM: To investigate the effects of Shenmai injection (SM) on the expression of Fas/FasL mRNA during pulmonary ischemia and reperfusion injury (PIRI) in rabbits.METHODS: Lung ischemia and reperfusion animal orthotopic model was established in vivo.The rabbits were randomly divided into three groups:sham operated group (sham, n=10), ischemia-reperfusion group (I-R, n=10) and I-R +Shenmai injection group (SM, n=10).The changes in several parameters, which included Fas/FasL mRNA locating expression, apoptotic index (AI), wet to dry ratio of lung tissueweight(W D) and index of quantitative assessment of histologic lung injury(IQA), and the histomorphology under light and electron microscope, were evaluated at 3 h after reperfusion in lung tissue.RESULTS: Compared with I-R group, the Fas/FasL mRNA slightly expressed in intima and extima of small pulmonary artery, pulmonary vein, and alveolar epithelium, bronchiole epithelia in SM group.The values of AI, W D and IQA were significantly lower than those in I-R group at 3 h after reperfusion in lung tissue (P <0.01 and P <0.05), and the changes of lung tissue in histomorphology were less markedly in SM group.CONCLUSION: Shenmai injection has notable protective effects on PIRI in rabbits, which may related with inhibition on Fas/FasL mRNA expression in lung tissue and decreasing apoptosis.

Key words: reperfusion injury, lung, Fas/FasL, apoptosis, shenmai injection

中图分类号:

R965.2

王俊, 林丽娜, 王万铁, 金立达, 邱晓晓, 戴雍月, 王青. 参麦注射液对肺缺血-再灌注损伤时Fas/FasL 表达的影响[J]. 中国临床药理学与治疗学, 2008, 13(12): 1355-1359.

WANG Jun, LIN Li-na, WANG Wan-tie, JIN Li-da, QIU Xiao-xiao, DAI Yong-yue, WANG Qin. Effects of Shenmai injection on the expression of Fas/FasL mRNA in pulmonary injury induced by ischemia-reperfusion in rabbits[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(12): 1355-1359.

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