生长相关癌基因α在大鼠哮喘中的表达意义及地塞米松对其的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (4): 396-400.

生长相关癌基因α在大鼠哮喘中的表达意义及地塞米松对其的影响

罗丽丹¹, 金小红², 童夏生³, 王恩智³, 郭海渊³, 阮正英³, 叶辉⁴

¹温岭市第四人民医院儿科,温岭317511,浙江;
²台州医院儿科,台州317000,浙江;
³温岭市第三人民医院儿科,温岭317523,浙江;
⁴台州市第人民医院儿科,台州318020,浙江

收稿日期:2008-01-03 修回日期:2008-03-26 出版日期:2008-04-26 发布日期:2020-10-12
通讯作者: 童夏生,男,硕士,副主任医师,研究方向:儿科哮喘。Tel:13758688128 E-mail:xshtzg@163.com
作者简介:罗丽丹,女,学士,主治医师,研究方向:儿科呼吸。Tel:13173790091 E-mail:JJP8203312@163.com
基金资助:
浙江省卫生厅基金资助项目(2007-B238);温岭市科技局基金资助项目(2006-53)

Expression of Grouth- Related Oncogen-a protein in rat asthma model and effects of dex amethasone

LUO Li-dan¹, JIN Xiao-hong², TONG Xia-sheng³, WANG En-zhi³, GUO Hai-yuan³, RUAN Zheng-ying³, YE Hui⁴

¹ Department of Pediatrics, Fourth Peple' s Hospital of Wenling,Wenlin 317511, Zhejiang, China;
² Department ofPediatrics, Taizhou Hospital, Taihou 317000,Zhgjiang, China;
³ Dpartment of Pediatrics, Third People' s Hospital of Wenling City,Wenling 317523,Zhejiang, China;
⁴ Department of Peliatrics, First People's Hospital of Taizhou, Taizhou 317000, Zhgjiang, China

Received:2008-01-03 Revised:2008-03-26 Online:2008-04-26 Published:2020-10-12

摘要/Abstract

摘要： 目的: 观察大鼠哮喘生长相关癌基因α(GROα)蛋白的表达水平及地塞米松对其的影响,探讨GROα和中性粒细胞(PMN)的相关性。方法: 采用大鼠哮喘模型,随机分成哮喘组、正常对照组、地塞米松治疗组,分离纯化血PMN,免疫组化法测定支气管腔和血PMN中GROα蛋白的表达水平,计数肺组织PMN数目。结果: 哮喘组(0.138±0.009,OD值)和地塞米松治疗组(0.105±0.012,OD值)支气管腔的GROa蛋白的平均吸光度值均显著高于正常对照组(0.077±0.010,OD值)(P<0.01),地塞米松治疗组GROa蛋白的平均吸光度值显著低于哮喘组(P<0.01)。哮喘组(0.211±0.017,OD值)和地塞米松治疗组(0.128±0.012,OD值)血PMN的GROα蛋白的平均吸光度值均显著高于正常对照组(0.081±0.008,OD值)(P<0.01),地塞米松治疗组GROα蛋白的平均吸光度值显著低于哮喘组(P<0.01)。三组PMN计数两两比较差异均存在显著统计学意义,正常对照组(7±2)<哮喘组(26士6)<地塞米松治疗组(202±30)(P<0.05或P<0.01)。GROα蛋白的表达水平与肺组织PMN计数在哮喘组和正常对照组中呈显著正相关(n=19,r=0.865,K0.01)。结论: 哮喘时支气管腔和PMN的GROa蛋白的表达水平及肺组织PMN计数增加,它们参与了哮喘发病的炎症过程;地塞米松部分通过抑制GROa蛋白的表达从而减轻气道炎症,但能加剧PMN在肺组织中聚集;GROa蛋白可能与PMN在肺组织中聚集密切相关。

Abstract: AIM: To observe the expressions of Grouth-Related Oncogen-a(GROa) in rat asthma model and effects of dexamethasone. METHODS: In this experiment, the rat model of asthma were randomly divided into three groups on average, incuding asthma group，control group and dexamethasone treated group.Blood PMN were isolated and purified.The expressions of GROa protein in bronchial wall and in blood PMN were detected by immunohistochemical method. Numbers of PMN were calculated in lung tissue. RESULTS: Levels of GROa protein in asthma group(0.138±0.009,Optical density) and in dexamethasone treated group(0.105±0.012, Optical density)were significant higher than those in control group(0.077±0.010，Optical density) (P<0.01) in bronchial wall. And levels of GROa protein in dexamethasone treated group were significantly decreased compared to asthma gnoup(PK 0.01). While in blood PMN，The expressions of GROa protein in asthma group(0.211土0.017, Optical density) and in dexamethasone treated goup(0.128±0.012, Optical densirty) were significant higher than those in control group(0.081±0.008，Optical density)(P<0.01). And levels of GROa protein in dexamethasone treated group were significantly decreased compared to asthma group(P<0.01). And the levels of G ROa protein in bronchial wall and blood PMN were significant different between dexamethasone treated group and those in asthma group(P<0.01). There numbers of PMN in lung tissue were significant different among three goups, in control group (7±2)< in asthma group(26±6)CONCLUSION: In bronchial wall and in blood PMN，levels of GROa protein were increased in rat asthma. And numbers of PMN in lung tissue were increased either. They are involved in inflammation of rat asthma model. The decreased inflammation of asthma by dexamethasone maybe via decreased synthesis of GROa protein. But dexamethasone can increasing PMN influxing into lung tissue. The influx of PMN into lung tissue may be corelated with GROa protein.

Key words: asthma, rat, GROa protein, polymorphonuclear leukocyte, immunohistochemical method, dex amet hasone

中图分类号:

R965.2

罗丽丹, 金小红, 童夏生, 王恩智, 郭海渊, 阮正英, 叶辉. 生长相关癌基因α在大鼠哮喘中的表达意义及地塞米松对其的影响[J]. 中国临床药理学与治疗学, 2008, 13(4): 396-400.

LUO Li-dan, JIN Xiao-hong, TONG Xia-sheng, WANG En-zhi, GUO Hai-yuan, RUAN Zheng-ying, YE Hui. Expression of Grouth- Related Oncogen-a protein in rat asthma model and effects of dex amethasone[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(4): 396-400.

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