哮喘大鼠中性粒细胞基质金属蛋白酶9和血清基质金属蛋白酶抑制剂-1的表达及地塞米松对其的影响

中国临床药理学与治疗学 ›› 2008, Vol. 13 ›› Issue (8): 870-873.

哮喘大鼠中性粒细胞基质金属蛋白酶9和血清基质金属蛋白酶抑制剂-1的表达及地塞米松对其的影响

周华斐¹, 王昕昕¹, 童夏生², 李绍波¹, 王恩智², 阮正英², 叶辉³, 金小红¹, 郭海渊²

¹台州医院儿科, 台州317500, 浙江;
²温岭市第三人民医院儿科, 温岭317523, 浙江;
³台州市第一人民医院, 台州317500, 浙江

收稿日期:2008-03-31 修回日期:2008-08-12 出版日期:2008-08-26 发布日期:2020-10-12
通讯作者: 童夏生, 男, 硕士, 副主任医师, 研究方向:儿科哮喘。Tel:13758688128　E-mail:xshtzg@163.com
作者简介:周华斐, 女, 学士, 副主任医师, 研究方向:儿科呼吸。Tel:13968683696　E-mail:wldsyy@163.com
基金资助:
浙江省卫生厅基金资助项目(2007-B238)

Expression levels of matrix metalloproteinase-9 in blood polymorphonuclear leucocyte and serum TIMP-1 and the effect of dexamethasone on them in rat asthma models

ZHOU Hua-fei¹, WANG Xing-xing¹, TONG Xia-sheng², LI Shao-bo¹, WANG En-zhi², RUAN Zheng-ying², YI Hui³, JING Xiao-hong¹, GUO Hai-yuan²

¹Department of Pediatrics, Taizhou Hospitaly, Taizhou 317000, Zhejiang, China;
²Department of Pediatrics, Third People's Hospital of Wenling City, Wenling 317523, Zhejiang, China;
³Department of Pediatrics, Taizhou First Hospitaly, Taizhou 317000, Zhejiang, China

Received:2008-03-31 Revised:2008-08-12 Online:2008-08-26 Published:2020-10-12

摘要/Abstract

摘要： 目的:观察基质金属蛋白酶9(MMP-9)在哮喘大鼠血中性粒细胞(PMN)中的表达及地塞米松对其的影响, 探讨血PMN 中MMP-9和基质金属蛋白酶抑制剂-1(TIMP-1)的相关性。方法:采用哮喘大鼠模型, 随机分成哮喘组、正常对照组、地塞米松治疗组, 对血PMN 进行分离纯化, 免疫细胞化学法检测MMP-9的表达水平, ELISA 法检测血清TIMP-1的蛋白浓度。结果:哮喘组(0.196±0.011, OD 值)和地塞米松治疗组(0.135±0.008,OD 值)中MMP-9的表达水平均显著高于正常对照组(0.100±0.010, OD 值)(P<0.01), 地塞米松治疗组MMP-9的表达水平显著低于哮喘组(P<0.01)。哮喘组[(34.96±4.54)ng/mL] 血清TIMP-1的表达水平分别显著高于正常对照组[(26.14±3.43)ng/mL] 和地塞米松治疗组[(29.89±3.43)ng/mL] (均P<0.01), 地塞米松治疗组TIMP-1的表达水平显著高于正常对照组(P<0.05)。MMP-9与TIMP-1的表达水平呈显著正相关(n=29, r=0.741, P<0.01)。结论:PMN 能合成MMP-9, 且哮喘时其合成水平增加。PMN 可能部分通过MMP-9起作用来参与哮喘的炎症过程, 其合成功能可以被地塞米松所抑制。MMP-9及其抑制物TIMP-1共同参与哮喘的发病机制。

关键词: 哮喘, 基质金属蛋白酶9, 中性粒细胞, 基质金属蛋白酶抑制剂-1, 免疫细胞化学, 大鼠

Abstract: AIM:To investigate the expression of matrix metalloproteinase-9(MMP-9)in blood polymorphonuclear leukocyte(PMN)and the effect of dexamethasone on them in rat asthma models.To explore the relationship between MMP-9 in PMN and its inhibitor matrix metallo-proteinase inhibitor-1(TIMP-1). METHODS:The rat models of asthma were randomly divided into asthma group (group A), control group (group C)and dexamethasone treated group (group C).Blood PMN was isolated and purified.The expression of MMP-9was detected by immunocytochemical method in blood PMN.The concentration of TIMP-1protein was detected by ELISA.RESULTS:The levels of MMP-9 in PMN in group A (OD=0.196± 0.011)and in group D(OD=0.135±0.008)were significantly higher than those in group C(OD=0.100±0.010)(P<0.01).The levels of MMP-9 in PMN in group D were significantly lower than those in group A.The levels of TIMP-1protein in group A[(34.96± 4.54)ng/mL] were significantly higher than those in group C [(26.14±3.43)ng/mL] and in group D [(29.89±3.43)ng/mL] (P<0.01).The levels of TIMP-1protein in group D were significantly higher than those in group C(P<0.05).There was significant positive correlation between bloodMMP-9 in PMN protein expression level and concentration of TIMP-1protein(n=29, r=0.741, P<0.01).CONCLUSION:MMP-9 protein can be synthesized by PMN, and expression levels of MMP-9 are increased in rat asthma.PMN are involved in asthmatic inflammation and maybe by increasing synthesis of MMP-9.But this function of PMN can be inhibited by dexamethasone. MMP-9 and its inhibitor TIMP-1may take part in the pathogenesis of asthma exacerbation.

Key words: asthma, matrix metalloproteinase-9, polymorphonuclear leukocyte, TIMP-1, immunocytochemical method, rat

中图分类号:

R965.2

周华斐, 王昕昕, 童夏生, 李绍波, 王恩智, 阮正英, 叶辉, 金小红, 郭海渊. 哮喘大鼠中性粒细胞基质金属蛋白酶9和血清基质金属蛋白酶抑制剂-1的表达及地塞米松对其的影响[J]. 中国临床药理学与治疗学, 2008, 13(8): 870-873.

ZHOU Hua-fei, WANG Xing-xing, TONG Xia-sheng, LI Shao-bo, WANG En-zhi, RUAN Zheng-ying, YI Hui, JING Xiao-hong, GUO Hai-yuan. Expression levels of matrix metalloproteinase-9 in blood polymorphonuclear leucocyte and serum TIMP-1 and the effect of dexamethasone on them in rat asthma models[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2008, 13(8): 870-873.

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