哮喘大鼠硫化氢含量与胱硫醚-γ-裂解酶mRNA 表达的实验性研究

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (1): 20-24.

哮喘大鼠硫化氢含量与胱硫醚-γ-裂解酶mRNA 表达的实验性研究

方丽¹, 李昌崇², 童夏生³, 王华芳¹, 王招定¹

¹诸暨市人民医院儿科, 绍兴 311800, 浙江;
²温州医学院附属育英儿童医院呼吸科, 温州 325003, 浙江;
³台州中西医结合医院儿科, 温岭 317523, 浙江

收稿日期:2008-12-22 修回日期:2009-01-05 出版日期:2009-01-26 发布日期:2020-10-27
通讯作者: 童夏生,男, 硕士, 副主任医师, 研究方向:儿科呼吸。Tel:13758688128 E-mail:xshtzg@163.com
作者简介:方丽, 女, 硕士, 主治医师, 研究方向:儿科呼吸。Tel:13567578335 E-mail:zjwz2fl@126.com
基金资助:
浙江省温岭市科技局基金资助项目(2007-55)

Experimental research about levels of endogenous hydrogen sulfide and cystathionine-γ-lyase mRNA in asthmatic rats

FANG Li¹, LI Chang-chong², TONG Xia-sheng³, WANG Hua-fang¹, WANG Zhao-ding¹

¹Department of Pediatrics, People' s Hospital of Zhuji, Shaoxing 311800, Zhejiang, China;
²Respitory Department, theYuying Children' s Hospital Affiliated to Wenzhou Medical College, Wenzhou 325027, Zhejiang, China;
³Taizhou Integrated Traditional and Western Medicine Hospital, Wenling 317523, Zhejiang, China

Received:2008-12-22 Revised:2009-01-05 Online:2009-01-26 Published:2020-10-27

摘要/Abstract

摘要： 目的: 观察哮喘大鼠硫化氢(H₂S)含量与胱硫醚-γ- 裂解酶(CSE)mRNA 的表达, 探讨H₂S和CSE 在哮喘发病机制中的作用。方法: 采用哮喘大鼠模型, 将30 只SD 大鼠随机分成哮喘组、正常对照组、地塞米松治疗组, 分光光度法测定血浆H₂S 的含量,RT-PCR 法测定肺组织CSE mRNA 的表达水平。结果: 哮喘组血浆H₂S 的含量显著低于正常对照组[(61±16 vs 84±15)μmol/L, P<0.01], 地塞米松治疗组[(73±16)μmol/L] 与哮喘组和正常对照组相比无统计学差异(均P>0.05)。哮喘组肺组织CSE mRNA 的表达水平显著低于正常对照组(0.14±0.02 vs 0.46±0.05, P<0.01), 地塞米松治疗组(0.33±0.04)显著性低于正常对照组但高于哮喘组(P均<0.01)。血浆H₂S 的含量和肺组织CSE mRNA 的表达水平呈显著正相关(n=30, r=0.504, P<0.01)。结论: 哮喘大鼠血浆H₂S 含量与肺组织CSE mRNA 的表达水平均下降, 它们可能参与了哮喘的炎症过程。地塞米松改善哮喘炎症的机制可能部分通过H₂S/CSE 体系。

关键词: 哮喘, 大鼠, 硫化氢, 胱硫醚-γ-裂解酶, 地塞米松

Abstract: AIM: To investigate the levels of endogenous hydrogen sulfide(H₂S)and cystathionine-γ-lyase(CSE)mRNA of acute asthma, and to explore the functions of H2S and CSE in mechanism of asthma exacerbation.METHODS: Thirty male SD rats were randomly divided into three groups, named asthma group,control group and dexamethasone treated group.The contents of H₂S were detected in the blood plasma by spectrophotometry.The levels of CSE mRNA in the lung tissues were detected by RT-PCR.RESULTS: The contents of H₂S in plasma of asthma group(61±16)μmol/L were significantly decreased than those in control group[(84±15)μmol/L, P<0.01].But there were nosignificance between dexamethasone treated group[(73±16)μmol/L] and control group(P>0.05), and there were nonsignificance between dexamethasone treated group and asthma group either(P>0.05).Levels of CSE mRNA in the lung tissues of asthma group(0.14±0.02 vs 0.33±0.04)were significantly decreased than those in control group (0.46±0.05)(P<0.01).And in dexamethasone treated group(0.33±0.04), levels of CSE mRNA in the lung tissues were significantly increased than those in asthma group(P<0.01), but were significantly decreased than those in control group(P<0.01).There were significantly positive correlation between contents of H₂S in the blood plasma and levels of CSE mRNA in the lung tissues(n=30, r=0.504, P<0.01).CONCLUSION: The contents of H₂S in the blood plasma and levels of CSE mRNA in the lung tissues are decreased in asthmatic rats.They possibly participate in the inflammation process of asthma.Dexamethasone reducing the airway inflammation of asthma is possibly in part to the system of H₂S CSE.

Key words: asthma, rat, hydrogen sulfide, cystathionine-γ-lyase, dexamethasone

中图分类号:

R965.2

方丽, 李昌崇, 童夏生, 王华芳, 王招定. 哮喘大鼠硫化氢含量与胱硫醚-γ-裂解酶mRNA 表达的实验性研究[J]. 中国临床药理学与治疗学, 2009, 14(1): 20-24.

FANG Li, LI Chang-chong, TONG Xia-sheng, WANG Hua-fang, WANG Zhao-ding. Experimental research about levels of endogenous hydrogen sulfide and cystathionine-γ-lyase mRNA in asthmatic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(1): 20-24.

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