芍灵消增贴对膝骨关节炎患者血清NO 与外周血单个核细胞的金属蛋白酶9 mRNA 表达的影响

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (3): 322-327.

芍灵消增贴对膝骨关节炎患者血清NO 与外周血单个核细胞的金属蛋白酶9 mRNA 表达的影响

李恒¹, 沈霖¹, 李丽琴², 帅波¹, 郭向飞¹

¹华中科技大学同济医学院附属协和医院中西医结合骨科;
²华中科技大学生命科学与技术学院, 武汉430022, 湖北

收稿日期:2008-08-21 修回日期:2008-08-21 发布日期:2020-10-27
通讯作者: 沈霖, 男, 教授, 主要从事骨组织方面及骨与关节疾病的研究。Tel:13986144898 E-mail:shenlinhb @yahoo.com.cn
作者简介:李恒, 男, 在读博士, 研究方向:骨与关节疾病的研究。Tel:15527008418 E-mail:lihengunion @yahoo.com.cn
基金资助:
湖北省卫生厅资助课题(鄂卫发2004-79)

Effects of Shaolingxiaozeng Tie on serum levels of NO and MMP-9mRNA in peripheral blood mononuclear cell on knee osteoarthritis patients

LI Heng¹, SHEN Lin¹, LI Li-qin², SHUAI Bo¹, GUO Xiang-hei¹

¹Department of Combination of TCM with Western Medicine, Union Hospital, TongjiMedical College, Huazhong University of Technology and Science;
²Life Science and Technology College, Wuhan 430022, Hubei, China

Received:2008-08-21 Revised:2008-08-21 Published:2020-10-27

摘要/Abstract

摘要： 目的观察芍灵消增贴对膝骨关节炎(OA)患者血清NO 与外周血单个核细胞(PBMC) 的基质金属蛋白酶9(MMP-9) mRNA 表达的影响, 探讨其疗效机理。 方法: 采用随机、双盲、安慰剂平行对照临床研究方法, 并设立健康对照组。随机选择健康志愿者共21 例, 作为健康组;将门诊膝OA患者共60 例, 随机进入试验组39 例( 芍灵消增贴) 和对照组21 例( 安慰剂) , 共治疗2 周。治疗前后各采取空腹血清测定NO 与PBMC 的MMP-9mRNA 表达, 并采用Lequense 指数作为疗效评分标准, 观察治疗前后膝关节症状变化包括休息痛、运动痛、压痛、肿胀、晨僵、行走能力的改善程度。 结果: 试验组与对照组血清NO 、PBMC 的MMP-9mRNA 基础值均高于健康组, 且差异有统计学意义(P<0.05) ;治疗2 周后, 试验组血清NO 、PBMC的MMP-9 mRNA 及Lequesne 指数值低于基础值,且差异有统计学意义(P<0.05),NO 、MMP-9 表达量与休息痛、关节肿胀、行走能力及总Lequesne 指数呈显著正相关。结论芍灵消增贴作用机理是通过各种途径下调血清NO 的浓度和抑制PBMC的MMP-9 mRNA 表达, 从而抑制软骨细胞凋亡和软骨基质的降解, 达到治疗和缓解OA 临床症状的目的。

关键词: 骨关节炎, NO, 基质金属蛋白酶-9

Abstract: AIM: To observe the effects of Shaolingxiaozeng Tie on serum levels of nitric oxide (NO) and matrix metalloproteinase-9(MMP-9) mRNA in peripheral blood mononuclear cell(PBMC) on knee osteoarthritis (OA) patients and further to study its mechanism. METHODS: Healthy group (n =21) was established with a randomized, double-blind, placebo, clinical trial research method.Sixty outpatients with knee osteoarthritis were randomly divided into Shaolingxiaozeng Tie(n =39) and placebo (n =21) groups. The treatment lasted for 2 weeks.The serum levels of NO and MMP-9mRNA in peripheral blood mononuclear cell were measured before and after treatment.The changes of articular genu symptoms such as knee pain at rest and movement, tenderness, knee swelling, morning stiffness and the improvement of walking ability were scored by Lequesne's index before and after treatment. RESULTS: The serum levels of NO and MMP-9 mRNA in PBMC in Shaolingxiaozeng Tie and placebo groups were higher than those in healthy group, there were significant differences (P<0.05). After two weeks'treatment, the serum levels of NO and MMP-9 mRNA in PBMC and Lequesne's score in Shaolingxiaozeng Tie group were lower compared with the foundmental values, there were significant differences (P<0.05).There was signifcant positive correlation between the serum levels of NO and MMP-9 mRNA in PBMC and the knee pain at rest and movement, tenderness, knee swelling, morning stiffness, the walking ability and total Lequesne's score. CONCLUSION: Decreasing the concentration of serum NO and inhibiting the expression of MMP-9 mRNA in PBMC by various ways may be the action mechanisms of Shaolingxiaozeng Tie, so the clinical symptom of osteoarthritis is improved and relieved by inhibiting the apoptosis of chondrocytes and the degradation of cartilage matrix.

Key words: osteoarthritis, nitric oxide, matrix metalloproteinase-9

中图分类号:

R593

李恒, 沈霖, 李丽琴, 帅波, 郭向飞. 芍灵消增贴对膝骨关节炎患者血清NO 与外周血单个核细胞的金属蛋白酶9 mRNA 表达的影响[J]. 中国临床药理学与治疗学, 2009, 14(3): 322-327.

LI Heng, SHEN Lin, LI Li-qin, SHUAI Bo, GUO Xiang-hei. Effects of Shaolingxiaozeng Tie on serum levels of NO and MMP-9mRNA in peripheral blood mononuclear cell on knee osteoarthritis patients[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(3): 322-327.

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