PTEN在哮喘大鼠肺部的表达及地塞米松的干预

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (8): 872-876.

PTEN在哮喘大鼠肺部的表达及地塞米松的干预

徐慧, 戴元荣, 曾潍显, 何剑波, 夏晓东

温州医学院附属二院呼吸内科, 温州 325003, 浙江

收稿日期:2009-03-26 修回日期:2009-07-27 出版日期:2009-08-26 发布日期:2020-10-30
通讯作者: 戴元荣, 男, 硕士, 主任医师, 主要从事哮喘发病机理研究。Tel:0577-88879063 E-mail:daiyr@126.com.cn
基金资助:
浙江省科技计划项目(2006C33074)

Expression of PTEN and the intervention effect of dexamethasone in the asthma rat model

XU Hui, DAI Yuan-rong, ZENG Wei-xian, HE Jian-bo, XIA Xiao-dong

Second Affiliated Hospital of Wenzhou Medical College, Wenzhou325003, Zhejiang, China

Received:2009-03-26 Revised:2009-07-27 Online:2009-08-26 Published:2020-10-30

摘要/Abstract

摘要： 目的: 研究哮喘大鼠 PTEN 的表达和地塞米松(DXM) 对其表达的影响。方法: 24 只体重(200±10) g 的 SPF 级雄性 SD 大鼠, 随机分为 3组:正常对照组、哮喘组、地塞米松组。对支气管肺泡灌洗液(BALF) 进行细胞分类计数;应用双抗体夹心酶联免疫吸附试验法测定 BALF 中 IL-13浓度 ;采用免疫组化法和 Western blot 法分别检测PTEN 的表达及各组大鼠肺组织 PTEN 量的变化。结果: 正常对照组、地塞米松组支气管 PTEN 蛋白的表达分别为 7.87±0.80、1.80 ±0.23, 均高于哮喘组(0.92±0.23, 均为 P<0.01) , 其主要表达细胞是支气管上皮细胞;哮喘组大鼠 BALF 中 IL-13表达明显升高, 明显高于正常对照组;地塞米松组 BALF 中 IL-13 量明显下降, 与哮喘组比有统计学意义。哮喘组大鼠支气管 PTEN 蛋白与BALF 中的 IL-13 浓度呈显著负相关(r=-0.675,P<0.01) 。结论: 气道上皮细胞是 PTEN 主要表达细胞, 哮喘大鼠 PTEN 蛋白表达明显减少, PTEN能减少 Th 2 因子的表达;DXM 可以促进 PTEN 的表达, 可能为其抑制哮喘气道炎症形成的重要作用机制。

关键词: 哮喘, PTEN, 气道炎症, 地塞米松

Abstract: AIM: To study the expression of PTEN and the intervention effect of dexamethasone(DXM) in the asthma rat model .METHODS: Twenty-four (200 ±10) g SD rats were randomly divided into three groups:control group(Group C) , asthma group(Group A) and DXM group(Group DXM) .The lung tissue was sampled from the left lung .The bronchoalveolar lavage fluid (BALF) was collected from the right lung .The differential count of cells in BALF was measured.The concentration of IL-13 in BALF wasmeasured by Sand-wich ELISA .The expression of PTEN protein and the changes of PTEN level in lung homogenate of each groupwere detected by immunohistochemical and West-ern blot method. RESULTS: The expression of bron-chial PTEN protein in Group C and Group DXM were 7.87±0.80, 1.80±0.23 which were higher than that in Group A(0.92 ±0.23, all P<0.01) , and the bronchial epithelial cell was the main expression cell. The expression of IL-13 in BALF of Group A was sig-nificantly increased, and which was higher than that in Group C (P<0.01) , and which in Group DXM was significantly decreased, compared with Group A, there was significant difference .There were significantly negative correlations between the expression of PTEN protein in lung homogenate and the level of IL-13 in BALF(r =-0.675, P<0.01) .CONCLUSION: The PTEN chief expression cell was tracheal epithelial cell .The expressions of PTEN protein in Group A were decreased.PTEN could decrease the expression of Th₂ factor.Dexamethasone could promote the expression of PTEN,which may be an important mechanism of inhib-iting asthmatic airway inflammation formation.

Key words: asthma, PTEN, airway inflammation, dexamethasone

中图分类号:

R965.2

徐慧, 戴元荣, 曾潍显, 何剑波, 夏晓东. PTEN在哮喘大鼠肺部的表达及地塞米松的干预[J]. 中国临床药理学与治疗学, 2009, 14(8): 872-876.

XU Hui, DAI Yuan-rong, ZENG Wei-xian, HE Jian-bo, XIA Xiao-dong. Expression of PTEN and the intervention effect of dexamethasone in the asthma rat model[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(8): 872-876.

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