中性粒细胞激活蛋白-2在大鼠哮喘中性粒细胞中的表达及意义

中国临床药理学与治疗学 ›› 2009, Vol. 14 ›› Issue (8): 883-887.

中性粒细胞激活蛋白-2在大鼠哮喘中性粒细胞中的表达及意义

周华斐¹, 罗冬娇², 童夏生³, 郭海渊⁴, 阮正英⁴, 叶辉⁵

¹温州医学院附属台州医院儿科 ,台州 317000, 浙江;
²杭州师范大学钱江学院医学系,杭州 310012,浙江;
³台州市中西医结合医院儿科,附属浙江中医药大学第一临床医学;
⁴台州市中西医结合医院病理科 ,附属浙江中医药大学第一临床医学,温岭 317523,浙江;
⁵台州市第一人民医院儿科, 台州 317000, 浙江

收稿日期:2009-05-13 修回日期:2009-08-06 出版日期:2009-08-26 发布日期:2020-10-30
通讯作者: 童夏生,男, 硕士, 副主任医师, 研究方向:儿科呼吸。E-mail:xshtzg @163.com
作者简介:周华斐, 女, 本科, 副主任医师, 研究方向:儿科呼吸。E-mail:527287916@QQ.com
基金资助:
浙江省卫生厅基金资助项目(2007-B238); 温岭市科技局基金资助项目(2006-53)

Expression and significance of neutrophil-activating peptide-2 in asthma rat

ZHOU Hua-fei¹, LUO Dong-jiao², TONG Xia-sheng³, GUO Hai-yuan⁴, RUAN Zheng-ying⁴, YE Hui⁵

¹Department of Pediatrics, Taizhou Hospital , Affiliated to Wenzhou Medical College , Taizhou 317000 , Zhejiang ,China;
²Department of Medical Science , Qianjiang College , Hangzhou Normal University , Hangzhou 310012 ,Zhejiang , China; 3Department of Pediatrics , Taizhou Integrated Western and Traditional Chinese Medicine Hospital ,Affiliated to the First Clinical Medicine of Zhejiang Chinese Medical University; 4Department of Pathology , Taizhou Integrated Western and Traditional Chinese Medicine Hospital , Affiliated to the First Clinical Medicine of Zhejiang Chinese Medical University , Wenling317523 , Zhejiang , China;
⁵Department of Pediatrics, the First People's Hospital of Taizhou , Taizhou 317000 , Zhejiang , China

Received:2009-05-13 Revised:2009-08-06 Online:2009-08-26 Published:2020-10-30

摘要/Abstract

摘要： 目的: 观察大鼠哮喘中性粒细胞激活蛋白-2(NAP-2)蛋白在中性粒细胞(NEU)上的表达及地塞米松对其的影响, 探讨 NEU 参与哮喘发病的可能作用机制。方法: 采用大鼠哮喘模型 ,随机分成哮喘组、对照组、地塞米松治疗组, 分离纯化血NEU,免疫组织化学法测定支气管和血 NEU 中NAP-2 蛋白的表达水平。结果: 哮喘组NEU NAP-2 蛋白的平均光密度值(0.198±0.016)显著高于对照组值(0.079±0.015)(P<0.01);地塞米松治疗组的NEU NAP-2 蛋白的平均光密度值(0.135±0.021)显著低于哮喘组且高于对照组(P<0.01)。哮喘组支气管NAP-2蛋白的平均光密度值(0.226±0.050)显著高于对照组值(0.140±0.012)(P<0.01);地塞米松治疗组的支气管NAP-2蛋白的平均光密度值(0.175±0.028)显著低于哮喘组且高于对照组(P<0.01或 P<0.05)。NEU NAP-2 蛋白的表达水平与支气管肺泡灌洗液 NEU 计数呈显著正相关(n=29, r =0.334, P<0.05)。结论: 哮喘大鼠NEU NAP-2 蛋白的表达水平增加 ,NEU可能通过NAP-2参与哮喘发病的炎症过程;地塞米松部分通过抑制 NEU NAP-2 的表达从而减轻气道炎症 ,NAP-2可能与 NEU 在肺组织中聚集有关。

关键词: 哮喘, 中性粒细胞激活蛋白-2, 免疫组织化学, 中性粒细胞, 地塞米松

Abstract: AIM: To observe the effect of dexam-ethasone on the expression of neutrophil-activating pep-tide-2 (NAP-2)in neutrophil and explore the role of neutrophil in asthma rat .METHODS: In this experi-ment , the model rats of asthma were randomly divided into three groups on average, including asthma group, control group and dexamethasone treated group.Blood neutrophil were isolated and purified,the expression of NAP-2 protein in bronchial wall and in blood neutro-phil was detected by immunohistochemical assay .RESULTS: The expression of NAP-2 protein in blood neu-trophil from asthma group (0.198 ±0.016, optical density)was significant higher than that from control group (0.079 ±0.015, optical density)(P<0.01). And the expression of NAP-2 protein in blood neutro-phil from dexamethasone treated group (0.135 ± 0.021, optical density)was lower than that from asth-ma group, but was higher than that from control group (all P<0.01).While in bronchial wall , the expression of NAP-2 protein in asthma group (0.226 ±0. 050, optical density)was significant higher than that in control group (0.140±0 .012, optical density)(P<0.01).And the expression of NAP-2 protein in dex-amethasone treated group (0.175 ±0.028, optical density)was lower than that in asthma group, but was higher than that in control group (P<0.01).There were significant positive correlation between NAP-2 protein expression in blood neutrophil and the number of NEU in bronchoalveolar lavage fluid(n =29, r = 0.334, P<0.05).CONCLUSION: The levels of NAP-2 protein in blood neutrophil were increased in rat asthma .Neutrophil may be involved in asthma inflam-mation via NAP-2.The decreased inflammation of asth-ma by dexamethasone may partly depend on suppressing the synthesis of NAP-2 protein, which may be correlat-ed with the influx of neutrophil into lung tissue .

Key words: asthma, neutrophil-activating peptide-2, immunohistochemical method, neutrophil, dexamethasone

中图分类号:

R965.2

周华斐, 罗冬娇, 童夏生, 郭海渊, 阮正英, 叶辉. 中性粒细胞激活蛋白-2在大鼠哮喘中性粒细胞中的表达及意义[J]. 中国临床药理学与治疗学, 2009, 14(8): 883-887.

ZHOU Hua-fei, LUO Dong-jiao, TONG Xia-sheng, GUO Hai-yuan, RUAN Zheng-ying, YE Hui. Expression and significance of neutrophil-activating peptide-2 in asthma rat[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2009, 14(8): 883-887.

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