miRNA在糖尿病中的研究进展

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (2): 214-218.

miRNA在糖尿病中的研究进展

龚志成^1,2, 黄琼¹, 刘昭前¹

¹中南大学临床药理研究所,湖南省遗传药理学重点实验室, 长沙 410078,湖南;
²中南大学湘雅医院药剂科,长沙 410008,湖南

收稿日期:2009-12-23 修回日期:2010-01-27 出版日期:2010-02-26 发布日期:2020-09-18
通讯作者: 刘昭前,男, 博士,教授,博士生导师,研究方向:遗传药理学。Tel: 0731-84805380 E-mail: liuzhaoqian63@126.com
作者简介:龚志成,男,博士,副主任药师,研究方向:遗传药理学。Tel: 0731-84327317 E-mail: gongzhicheng2003@yahoo.com.cn
基金资助:
国家自然科学基金项目(30572230, 30873089);湖南省自然科学基金重点项目 (08JJ3058);湖南省“芙蓉学者计划特聘教授”(湘教通[2006]312号)

Research progress of miRNA in diabetes

GONG Zhi-cheng^1,2, HUANG Qiong¹, LIU Zhao-qian¹

¹ Institute of Clinical Pharmacology, Hunan Key Laboratory of Pharmacogenetics, Central South University, Changsha 410078, Hunan,China;
² Department of Pharmacy, The XiangYa Hospital of XiangYa Medical School, Central South University, Changsha 410078, Hunan,China

Received:2009-12-23 Revised:2010-01-27 Online:2010-02-26 Published:2020-09-18

摘要/Abstract

摘要： 已发现大量微小RNA(microRNA, miRNA)调节编码糖尿病相关蛋白基因的表达。miRNA 表达量或功能的变异与多种疾病相关。已有研究报道miRNA与糖尿病相关,miRNA参与调节胰岛β细胞的功能且糖尿病状态下组织内miRNA会发生变化。miRNA成为糖尿病治疗的潜在靶点。本文就近年来miRNA在糖尿病方面的研究进展进行综述。

关键词: 微小RNA, 糖尿病, 基因表达, 胰岛, RNA干扰

Abstract: The discovery hundreds of small RNA molecules, called microRNA, with the potential to modulate the expression of the majority of the protein-coding genes has revolutionized in the diabetes field. Moreover, alterations in the level or function of microRNA are associated with an increasing number of diseases. We present evidence for the involvement of microRNA in diabetes mellitus, by outlining the contribution of these microRNA molecules in the control of pancreatic β-cell functions and by reviewing recent studies reporting changes in microRNA expression in tissues. MicroRNA holds great potential as therapeutic targets. In this article, the researches of miRNA in diabetes and their potentialfor molecular therapy were summarized.

Key words: miRNA, Diabetes, Gene expression, Pancreatic islet, RNA interference

中图分类号:

R968

龚志成, 黄琼, 刘昭前. miRNA在糖尿病中的研究进展[J]. 中国临床药理学与治疗学, 2010, 15(2): 214-218.

GONG Zhi-cheng, HUANG Qiong, LIU Zhao-qian. Research progress of miRNA in diabetes[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(2): 214-218.

参考文献

[1] Lagos-Quintana M, Rauhut R, Lendeckel W, et al. Identification of novel genes coding for small expressed rnas[J]. Science, 2001,294(5543):853-858.
[2] Yanaihara N, Caplen N, Bowman E, et al. Unique microrna molecular profiles in lung cancer diagnosis and prognosis[J]. Cancer Cell, 2006,9(3):189-198.
[3] Berezikov E, Guryev V, van de Belt J, et al. Phylogenetic shadowing and computational identification of human microrna genes[J]. Cell, 2005,120(1):21-24.
[4] Bernstein E, Caudy AA, Hammond SM, et al. Role for a bidentate ribonuclease in the initiation step of rna interference[J]. Nature, 2001,409(6818):363-366.
[5] Pillai RS. Microrna function: Multiple mechanisms for a tiny rna[J] ? Rna, 2005,11(12):1753-1761.
[6] Lee Y, Ahn C, Han J, et al. The nuclear rnase iii drosha initiates microrna processing[J]. Nature, 2003,425(6956):415-419.
[7] Bartel DP. Micrornas: Target recognition and regulatory functions[J]. Cell, 2009,136(2):215-233.
[8] Reinhart BJ, Slack FJ, Basson M, et al. The 21-nucleotide let-7 rna regulates developmental timing in caenorhabditis elegans[J]. Nature, 2000,403(6772):901-906.
[9] Lee RC, Feinbaum RL, Ambros V, et al. Elegans heterochronic gene lin-4 encodes small rnas with antisense complementarity to lin-14[J]. Cell, 1993,75(5):843-854.
[10] Poy MN, Eliasson L, Krutzfeldt J, et al. A pancreatic islet-specific microrna regulates insulin secretion[J]. Nature, 2004,432(7014):226-230.
[11] El Ouaamari A, Baroukh N, Martens GA, et al. Mir-375 targets 3'-phosphoinositide-dependent protein kinase-1 and regulates glucose-induced biological responses in pancreatic beta-cells[J]. Diabetes, 2008,57(10):2708-2717.
[12] Poy MN, Hausser J, Trajkovski M, et al. Mir-375 maintains normal pancreatic alpha- and beta-cell mass[J]. Proc Natl Acad Sci USA, 2009,106(14):5813-5818.
[13] Plaisance V, Abderrahmani A, Perret-Menoud V, et al. Microrna-9 controls the expression of granuphilin/slp4 and the secretory response of insulin-producing cells[J]. J Biol Chem, 2006,281(37):26932-26942.
[14] Lovis P, Gattesco S, Regazzi R. Regulation of the expression of components of the exocytotic machinery of insulin-secreting cells by micrornas[J]. Biol Chem, 2008,389(3):305-312.
[15] Baroukh N, Ravier MA, Loder MK, et al. Microrna-124a regulates foxa2 expression and intracellular signaling in pancreatic beta-cell lines[J]. J Biol Chem, 2007,282(27):19575-19588.
[16] Tang X, Muniappan L, Tang G, et al. Identification of glucose-regulated mirnas from pancreatic {beta} cells reveals a role for mir-30d in insulin transcription[J]. Rna, 2009,15(2):287-293.
[17] Lovis P, Roggli E, Laybutt DR, et al. Alterations in microrna expression contribute to fatty acid-induced pancreatic beta-cell dysfunction[J]. Diabetes, 2008,57(10):2728-2736.
[18] Lynn FC, Skewes-Cox P, Kosaka Y, et al. Microrna expression is required for pancreatic islet cell genesis in the mouse[J]. Diabetes, 2007,56(12):2938-2945.
[19] Kloosterman WP, Lagendijk AK, Ketting RF, et al. Targeted inhibition of mirna maturation with morpholinos reveals a role for mir-375 in pancreatic islet development[J]. PLoS Biol, 2007,5(8):e203.
[20] Bravo-Egana V, Rosero S, Molano RD, et al. Quantitative differential expression analysis reveals mir-7 as major islet microrna[J]. Biochem Biophys Res Commun, 2008,366(4):922-926.
[21] Correa-Medina M, Bravo-Egana V, Rosero S, et al. Microrna mir-7 is preferentially expressed in endocrine cells of the developing and adult human pancreas[J]. Gene Expr Patterns, 2009,9(4):193-199.
[22] Bradley SP, Rastellini C, da Costa MA, et al. Gene silencing in the endocrine pancreas mediated by short-interfering rna[J]. Pancreas, 2005,31(4):373-379.
[23] Brummelkamp TR, Bernards R, Agami R. Stable suppression of tumorigenicity by virus-mediated rna interference[J]. Cancer Cell, 2002,2(3):243-247.
[24] Snove O, Rossi JJ. Expressing short hairpin rnas in vivo[J]. Nat Methods,2006,3(9):689-695.
[25] Zeng Y, Cai X, Cullen BR. Use of rna polymerase ii to transcribe artificial micrornas[J]. Methods Enzymol, 2005,392:371-380.
[26] Chen X, Ba Y, Ma L, et al. Characterization of micrornas in serum: A novel class of biomarkers for diagnosis of cancer and other diseases[J]. Cell Res, 2008,18(10):997-1006.
[27] Chin LJ, Slack FJ. A truth serum for cancer-micrornas have major potential as cancer biomarkers[J]. Cell Res,2008,18(10):983-984.
[28] Stanczyk J, Pedrioli DM, Brentano F, et al. Altered expression of microrna in synovial fibroblasts and synovial tissue in rheumatoid arthritis[J]. Arthritis Rheum, 2008,58(4):1001-1009.
[29] Xiao J, Luo X, Lin H, et al. Microrna mir-133 represses herg K⁺ channel expression contributing to qt prolongation in diabetic hearts[J]. J Biol Chem, 2007,282(17):12363-12367.