硫化氢供体硫氢化钠对肺缺血-再灌注损伤细胞凋亡的影响

中国临床药理学与治疗学 ›› 2010, Vol. 15 ›› Issue (5): 496-501.

硫化氢供体硫氢化钠对肺缺血-再灌注损伤细胞凋亡的影响

王兴民¹, 季海锋¹, 王万铁², 石璐², 贾旭广²

¹瑞安市中医院内科,瑞安 325200,浙江;
²温州医学院病理生理教研室,温州 325053, 浙江

收稿日期:2010-02-01 修回日期:2010-04-07 出版日期:2010-05-26 发布日期:2020-09-16
通讯作者: 王万铁,男,教授,主任医师,硕士研究生导师,主要研究方向:脏器缺血再灌注损伤。Tel: 0577-86689817 E-mail: wzwwt@tom.com
作者简介:王兴民,男,医学硕士,副主任医师,研究方向:大内科;脏器缺血再灌注损伤。Tel: 0577-65071662 E-mail: wxm0021@163.com
基金资助:
瑞安市科技局资助项目(20083075);温州市科技局项目(Y20090326)

Effects of sodium hydrosulfide on apoptosis in lung injury induced by ischemic and reperfusion in rats

WANG Xing-min¹, JI Hai-feng¹, WANG Wan-tie², SHI Lu², JIA Xu-guang²

¹Department of Internal Medicine, Ruian TCM Hospital, Ruian 325200, Zhejiang, China;
²Department of Pathophysiology, Wenzhou Medical College, Wenzhou 325053, Zhejiang, China

Received:2010-02-01 Revised:2010-04-07 Online:2010-05-26 Published:2020-09-16

摘要/Abstract

摘要： 目的: 探讨硫氢化钠(NaHS)对肺缺血-再灌注损伤(LIRI)时细胞凋亡的影响。 方法: 采用在体大鼠单肺原位肺缺血-再灌注模型。健康SD大鼠30只,随机均分成3组:假手术对照(Sham)组,肺缺血-再灌注(LIR)组,肺缺血再灌注+硫氢化钠(LIR+NaHS)组。各组大鼠实验结束后取肺组织,观察肺组织bcl2、bax蛋白的表达、凋亡指数(AI)、肺系数(LW/BW)、肺组织肺泡损伤率(IAR)及光镜、电镜下的肺组织形态学改变。结果: 在肺小血管内膜、肺泡上皮及细支气管上皮,LIR+NaHS组bcl-2蛋白表达及bcl-2/bax的比值显著高于LIR组(均P<0.01),bax蛋白的表达显著低于LIR组(均P<0.01);AI、LW/BW、IAR值显著低于LIR组(P<0.05);肺组织形态学异常改变不同程度减轻。结论: 硫化氢供体(NaHS)可上调bcl-2蛋白的表达,下调bax蛋白的表达,调控bcl-2/bax之间的平衡而减轻细胞凋亡,对LIRI具有保护作用。

关键词: 再灌注损伤, 肺, 细胞凋亡, 硫氢化钠

Abstract: AIM: To investigate the effect of sodium hydrosulfide (NaHS), the donor of H₂S, on apoptosis during lung ischemia and reperfusion injury (LIRI) in rats. METHODS: Single lung ischemia and reperfusion animal model were used in vivo. A total of 30 Sprague-Dawley(SD) rats were divided at random into 3 groups as follows: sham operation control (Sham) group, lung ischemia-reperfusion(LIR) group, and LIR-sodium hydrosulfide(LIR+NaHS) group. Each group consisted of 10 rats. Under anesthetized, all rats were underwented the procedure of operation as the following: opening the left chest and isolating the hilus of lung, through which a suture was passed. The Sham group didn't endure the pulmonary hila's ligation while the LIR group was subjected to a 30-minute ischemia and 120-minute reperfusion insult. The lung tissues sampled at the end of experiments were assayed for lung/body weight ratio (LW/BW).The tissue slides were also stained by immunohistochemistry (IHC) for bcl-2/bax to detect the expression of bcl-2/bax protein in lung and to analyze the optical density. The cell apoptosis in lung tissue were determined by TUNEL. The histological and ultrastructure changes were observed by light microscope and electron microscope. The injured alveoli rate (IAR) was counted as well. RESULTS: As compared with LIR group, in intima and extima of small pulmonary artery, alveoli, and bronchiole epithelia, the expression of bcl-2 proteins and the ratio of bcl-2/bax were increased, and the expression of bax protein was decreased in LIR+NaHS group. The values of AI, LW/BW and IAR showed significantly lower than that in LIR group (P<0.05). Meanwhile, abnormal changes of the lung tissue in morphologically were markedly lesseed in LIR-NaHS group. CONCLUSION: NaHS produces a notable protective effect on LIRI in rats by up-regulating bcl-2 protein expression, down-regulating bax protein expression in lung tissue and regulating the balance of bcl-2 and bax to decrease apoptosis.

Key words: Ischemia-reperfusion injury, Lung, Apoptosis, Sodium hydrosulfide

中图分类号:

R965.2

王兴民, 季海锋, 王万铁, 石璐, 贾旭广. 硫化氢供体硫氢化钠对肺缺血-再灌注损伤细胞凋亡的影响[J]. 中国临床药理学与治疗学, 2010, 15(5): 496-501.

WANG Xing-min, JI Hai-feng, WANG Wan-tie, SHI Lu, JIA Xu-guang. Effects of sodium hydrosulfide on apoptosis in lung injury induced by ischemic and reperfusion in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2010, 15(5): 496-501.

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