加味五味消毒饮提取物对糖尿病大鼠牙周组织的保护作用

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (11): 1223-1228.

加味五味消毒饮提取物对糖尿病大鼠牙周组织的保护作用

向敏^1,2, 张雅琴^1,2, 吴萍萍², 高振宇¹, 刘竞天¹, 顾瑶华¹, 王建梅¹

¹苏州卫生职业技术学院检验药学系,苏州 215009,江苏;
²苏州市检验医学生物技术重点实验室,苏州 215009,江苏

收稿日期:2012-08-28 修回日期:2012-10-10 发布日期:2012-12-04
作者简介:向敏,女,博士,副教授,研究方向:糖尿病血管病变及其分子机制。Tel: 0512-62690089 E-mail: xiangmin99@126.com
基金资助:
苏州市科技局应用基础研究计划资助课题(SYS201012)

Protection of modified wuweixiaoduyin extracts on the peridentalt of diabetic rats

XIANG Min^{1, 2}, ZHANG Ya-qin^{1, 2}, WU Ping-ping², GAO Zhen-yu¹, LIU Jing-tian¹, GU Yao-hua¹, WANG Jian-mei¹

¹Lab-pharmacy Department, Suzhou Health College, Suzhou 215009, Jiangsu, China;
²Key Laboratory of Biotechnology for Laboratory Medicine, Suzhou 215009, Jiangsu, China

Received:2012-08-28 Revised:2012-10-10 Published:2012-12-04

摘要/Abstract

摘要： 目的: 观察加味五味消毒饮提取物(modified wuweixiaoduyin extracts, MWE)对糖尿病(diabetic mellitus, DM)大鼠牙周组织的保护作用并探讨其初步机制。方法: 以栓线法结合注射链脲佐菌素(streptozotocin, STZ, 50 mg/kg)法制备糖尿病性牙周炎动物模型。实验分5组,正常对照组、模型组、氨基胍(Aminoguanidine, AG)阳性组(100 mg/kg)、MWE治疗小剂量组(20 g 生药/kg)和大剂量组(40 g 生药/kg),每组动物数10只。灌胃给药治疗6周后处死大鼠,测定空腹血糖值和糖化血红蛋白(GHb)水平,ELISA法测定血清晚期糖基化终产物(AGEs)浓度,牙周探针测量牙周袋深度,X光牙片机检查大鼠左上颌牙槽骨组织变化,免疫组化法检测牙龈组织AGEs受体(receptor for AGEs, RAGE)蛋白表达。结果: 治疗6周后,MWE几乎没有降血糖作用,但与模型组相比,治疗组牙周袋深度下降,GHb和AGEs水平降低(P<0.01), 骨小粱排列紊乱状况和牙槽骨水平吸收有所改善,RAGE蛋白表达水平也有下降。结论: MWE对糖尿病大鼠牙周组织具有保护作用,其机制可能与干扰AGEs-RAGE信号通路有关。

关键词: 加味五味消毒饮, 糖尿病, 牙周炎, 晚期糖基化产物, 晚期糖基化产物受体, 炎症反应

Abstract: AIM: To observe the protection effect of modified Wuweixiaoduyin extracts (MWE) on peridentalt of diabetic rats and investigate its possible mechanisms. METHODS: Diabetic periodontitis models were induced by combination periodontal ligation and injection of streptozotocin (STZ, 50 mg/kg). There were 5 experimental groups as followed: Normal, model groups, Aminoguanidine (AG) positive control groups (100 mg/kg), low dosage (20 g/kg, crude drug, the same below) and high dosage (40 g/kg) of MWE, with 10 rats in each group. Rats were killed after treatment for 6 weeks, the levels of fasting blood glucose and glycosylated hemoglobin (GHb) were determined, AGEs in serum was analyzed by ELISA. Depth of periodontal pocket was measured by periodontal probe. Left alveoli dentales maxillae were checked by dental X machine. In addition, expression of RAGE protein was detected by sABC methods.RESULTS: After treatment for 6 weeks, MWE didn't decrease blood glucose. However, compared with model group, MWE decreased depth of periodontal pocket, level of GHb and AGEs in serum (P<0.01). And derangement of Trabeculae bone and horizontal resorption in alveoli dentales maxillae were also ameliorated. In addition, expression of RAGE protein was reduced, too. CONCLUSION: MWE has protection effect on peridental of diabetic rats and the possible mechanism is related with interference AGEs-RAGE by MWE .

Key words: Modified wuweixiaoduyin, Diabetic mellitus, Periodontitis, AGEs, RAGE, Inflammatory reaction

中图分类号:

R965.2

向敏, 张雅琴, 吴萍萍, 高振宇, 刘竞天, 顾瑶华, 王建梅. 加味五味消毒饮提取物对糖尿病大鼠牙周组织的保护作用[J]. 中国临床药理学与治疗学, 2012, 17(11): 1223-1228.

XIANG Min, ZHANG Ya-qin, WU Ping-ping, GAO Zhen-yu, LIU Jing-tian, GU Yao-hua, WANG Jian-mei. Protection of modified wuweixiaoduyin extracts on the peridentalt of diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(11): 1223-1228.

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