LC-MS/MS测定大鼠血浆中乌头碱的浓度

中国临床药理学与治疗学 ›› 2012, Vol. 17 ›› Issue (4): 371-375.

LC-MS/MS测定大鼠血浆中乌头碱的浓度

王志琪^1,2, 景贤³, 曾嵘^1,2, 陈尧³, 谭志荣³

¹湖南中医药大学药学院,长沙 410208,湖南;
²湖南中医药大学干细胞中药调控与应用实验室,长沙 4102083,湖南;
³中南大学临床药理研究所,长沙 410078,湖南

收稿日期:2011-12-15 修回日期:2012-02-07 发布日期:2012-04-28
通讯作者: 谭志荣,男,副研究员,从事药物分析、遗传药代动力学研究。Tel: 0731-84805380 E-mail: tanzr@163.com
作者简介:王志琪,女,博士研究生,讲师,从事天然药物安全与有效性、药代动力学研究。Tel: 13017285185 E-mail: wzqx2004@126.com
基金资助:
湖南省教育厅科学研究项目(08B056),国家自然科学基金项目(81001471);湖南省重点学科建设项目资助(1007)

Determination of aconitine concentration in rats plasma by LC-MS/MS

WANG Zhi-qi^1,2, JING Xian³, ZENG Rong^1,2, CHEN Yao³, TAN Zhi-rong³

¹School of Pharmacy,Hunan University of Chinese Medicine,Changsha 410208,Hunan,China;
²Division of Stem Cell Regulation and Applicaton,Hunan University of Chinese Medicine, Changsha 410208,Hunan,China;
³Institute of Clinical Pharmacology, Central South University, Changsha 410078, Hunan, China

Received:2011-12-15 Revised:2012-02-07 Published:2012-04-28

摘要/Abstract

摘要： 目的: 建立LC-MS/MS法检测大鼠血浆中乌头碱的浓度。方法: 以维拉帕米作内标,血浆样品经MTBE液液萃取后,采用HyPURITY Cyano (150 mm×2.1 mm, 5 μm)柱分离,流动相为乙腈:10 mmol/L 甲酸铵=(70∶30,V/V),流速为 0.30 mL/min。然后采用电喷雾离子源(ESI源)正离子多反应监测(MRM)扫描分析,乌头碱和维拉帕米的离子选择通道分别为:m/z 646.4→586.4 和 455.2→164.9。结果: 乌头碱的线性范围为 9.3～2390 pg/mL,最低检测浓度为 9.3 pg/mL,日内和日间变异均<15%。结论: 本方法灵敏度高,适用于乌头碱在鼠内的药物代谢动力学研究。

关键词: LC-MS/MS, 乌头碱, 药代动力学

Abstract: AIM: To establish an LC-MS/MS method for determining the concentration of aconitine in rats plasma. METHODS: Verapamil was used as internal standard, the plasma samples extracted by MTBE were separated on a HyPURITY Cyano column (150 mm × 2.1 mm, 5 μm) with mobile phase contained acetonitrile and 10 mmol/L ammonium formate (70∶30, V/V) at flow rate of 0.30 mL/min. The electrospray ionization (ESI source) in positive ion multiple reaction monitoring (MRM) were used for scanning analysis, then, aconitine and verapamil ion selective channels were: m/z 646.4→586.4 and 455.2→164.9, respectively. RESULTS: The linear range for aconitine was 9.3-2390 pg/mL. The lower limit of quantitation was 9.3 pg/mL, and the relative standard deviation of intra-day and inter-day were less than 15%. CONCLUSION: The established method is suitable for pharmacokinetic study of aconitine.

Key words: LC-MS/MS, Aconitine, Pharmacokinetics

中图分类号:

R969.1

王志琪, 景贤, 曾嵘, 陈尧, 谭志荣. LC-MS/MS测定大鼠血浆中乌头碱的浓度[J]. 中国临床药理学与治疗学, 2012, 17(4): 371-375.

WANG Zhi-qi^1,2, JING Xian³, ZENG Rong^1,2, CHEN Yao³, TAN Zhi-rong³. Determination of aconitine concentration in rats plasma by LC-MS/MS[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2012, 17(4): 371-375.

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