还原型谷胱甘肽对2型糖尿病大鼠膈肌保护作用的研究

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (12): 1364-1368.

还原型谷胱甘肽对2型糖尿病大鼠膈肌保护作用的研究

杨丽娟^1,2, 刘磊³, 郭亚玲³, 高琴², 关宿东², 姜宝法¹

¹山东大学公共卫生学院流行病学教研室,济南 250012,山东;
²蚌埠医学院生理学教研室,蚌埠 233000,安徽;
³蚌埠医学院第一附属医院肾内科,蚌埠 233000,安徽

收稿日期:2013-05-24 修回日期:2013-11-19 出版日期:2013-12-26 发布日期:2014-01-04
通讯作者: 姜宝法,男,博士,教授,博士生导师,研究方向:慢性病流行病。E-mail: bjiang@sdu.edu.cn
作者简介:杨丽娟,女,学士,讲师,研究方向:慢性病学。Tel: 13655529911 E-mail: bbmcylj@163.com
基金资助:
国家自然科学基金(81000074);蚌埠医学院级课题(Byky1210)

Protective effect of prototype glutathione on the disfunction of diaphragmatic muscle in type 2 diabetic rats

YANG Li-juan^1,2, LIU Lei³, GUO Ya-ling³, GAO Qin², GUAN Su-dong², JIANG Bao-fa¹

¹School of Public Health, Shandong University,Jinan 250012,Shandong,China;
²Department of Physiology,Bengbu Medical College,Bengbu 233000, Anhui,China;
³Department of Nephropathy, the First Affiliated Hospital of Bengbu Medical College,Bengbu 233000, Anhui,China

Received:2013-05-24 Revised:2013-11-19 Online:2013-12-26 Published:2014-01-04

摘要/Abstract

摘要： 目的: 观察还原型谷胱甘肽(GSH)对2型糖尿病大鼠膈肌损伤的保护作用及其机制,并观察膈肌组织骨架蛋白nebulin和titin mRNA含量的变化。方法: 雄性SD大鼠随机分为正常对照组(CON组)、2型糖尿病模型组(DM组)和还原型谷胱甘肽治疗组(GSH组)。各组大鼠取离体膈肌条,测定其收缩力,测定膈肌组织中丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性,逆转录聚合酶链反应(RT-PCR)检测膈肌组织中骨架蛋白nebulin和titin的mRNA表达。结果: (1)与CON组比较,DM组大鼠膈肌单收缩力(P_t)、最大强直收缩力(P₀)、张力最大上升速率(+dT/dt_max)、张力最大下降速率(-dT/dt_max)明显降低(P<0.05);GSH组则高于DM组(P<0.05)。(2)与CON组相比,DM组大鼠膈肌组织MDA含量显著增高(P<0.05),SOD活性显著降低(P<0.01);GSH则能逆转该变化(P<0.05)。(3)DM组膈肌组织中nebulin和titin的mRNA表达显著低于CON组(P<0.01),而GSH组明显高于DM组(P<0.05)。结论: GSH通过提高膈肌组织骨架蛋白nebulin和titin的mRNA表达来增强2型糖尿病受损膈肌的收缩力,其机制可能与减轻膈肌组织氧化应激的损伤有关。

关键词: 还原型谷胱甘肽, 糖尿病, 膈肌, 大鼠

Abstract: AIM: To study the protective effect of reduced glutathione hormone (GSH) on the dysfunction of diaphragmatic muscle in type 2 diabetic rats and investigate the changes of diaphragm nebulin and titin mRNA expression.METHODS: Male SD rats were randomly divided into control group (CON group), type 2 diabetes mellitus group (DM group) and prototype glutathione treatment group (GSH group). The isolated diaphragm contractile force was measured, diaphragm SOD activity and MDA content, nebulin and titin mRNA levels in diaphragmatic muscle tissue were detected by reverse transcription polymerase chain reaction (RT-PCR).RESULTS: (1) P_t, P_o, +dT/dt_max and -dT/dt_max in rat diaphragm in DM group were significantly lower than those in CON group (P<0.05), which were higher in GSH group than those in DM group (P<0.05). (2) Diaphragm MDA content in DM group was increased significantly (P<0.05 vs CON), and SOD activity was significantly decreased (P<0.01 vs CON group); and these changes could be reversed by GSH (P<0.05 ). (3) The levels of nebulin and titin mRNA in DM group were significantly decreased (P<0.01 vs CON group), but increased in GSH group (P<0.05 vs DM group).CONCLUSION: GSH can increase the levels of nebulin and titin mRNA in diaphragm and improve diaphragm contractile force, which may be due to reduce oxidative stress injury.

Key words: Reduced glutathione hormone, Diabetes mellitus, Diaphragm, Rat

中图分类号:

R965.2

杨丽娟, 刘磊, 郭亚玲, 高琴, 关宿东, 姜宝法. 还原型谷胱甘肽对2型糖尿病大鼠膈肌保护作用的研究[J]. 中国临床药理学与治疗学, 2013, 18(12): 1364-1368.

YANG Li-juan, LIU Lei, GUO Ya-ling, GAO Qin, GUAN Su-dong, JIANG Bao-fa. Protective effect of prototype glutathione on the disfunction of diaphragmatic muscle in type 2 diabetic rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(12): 1364-1368.

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