针药结合对慢性脑缺血大鼠大脑皮质BDNF、TrkB及VEGF表达的影响

中国临床药理学与治疗学 ›› 2013, Vol. 18 ›› Issue (4): 376-381.

针药结合对慢性脑缺血大鼠大脑皮质BDNF、TrkB及VEGF表达的影响

张业贵, 丁艳霞, 侯良芹

皖南医学院人体解剖学教研室,芜湖 241002,安徽

收稿日期:2012-12-21 出版日期:2013-04-26 发布日期:2013-04-26
通讯作者: 侯良芹,女,博士,副教授,硕士生导师,研究方向:神经生物学。Tel: 0553-3932487 E-mail: hlq1227@sohu.com
作者简介:张业贵,男,硕士,讲师,研究方向:神经生物学。Tel: 13855346546 E-mail: zhangyegui025@163.com
基金资助:
安徽省高校省级优秀青年基金项目(2009SQRZ186);安徽省自然科学基金项目(1040606M162)

Effects of electroacupuncture combined with compound salviae miltiorrhizae on the expressions of BDNF, TrkB and VEGF in cortex of chronic cerebral ischemia rats

ZHANG Ye-gui, DIN Yan-xia, HOU Liang-qin

Department of Anatomy,Wannan Medical College,Wuhu 241002,Anhui,China

Received:2012-12-21 Online:2013-04-26 Published:2013-04-26

摘要/Abstract

摘要： 目的: 观察电针联合复方丹参对慢性脑缺血大鼠大脑皮质脑源性神经营养因子(BDNF)、酪氨酸激酶B(TrkB)及血管内皮生长因子(VEGF)表达的影响,探讨针药结合治疗脑缺血的可能机制。方法: 将SD雄性大鼠采用永久性双侧颈总动脉结扎术制备慢性脑缺血模型,将造模成功的32只大鼠随机分为模型组、复方丹参组、电针组、针药结合组,每组8只。复方丹参组给予复方丹参片 0.75 g/kg 灌胃,电针组选取百会、大椎穴给予电针刺激,持续 30 min,针药结合组联合采用复方丹参片灌胃和电针刺激,各组治疗均每天1次,连续5周。另用8只SD雄性大鼠作为正常对照组。采用免疫组织化学方法检测各组大鼠大脑皮质BDNF、TrkB及VEGF的表达。结果: 与正常组比较,模型组大鼠大脑皮质BDNF、TrkB 及VEGF表达明显减弱(P<0.01 或P<0.05);与模型组比较,复方丹参组、电针组、针药结合组大脑皮质BDNF、TrkB 及VEGF表达均明显增强(P<0.01 或P<0.05),并且针药结合组的表达高于复方丹参组和电针组(P<0.01 或P<0.05)。结论: 电针与复方丹参联合疗法对大鼠慢性缺血性脑损伤有保护作用,其机制可能与上调BDNF、TrkB及VEGF的表达有关。

关键词: 复方丹参, 电针, 脑缺血, 脑源性神经营养因子, 酪氨酸激酶B, 血管内皮生长因子

Abstract: AIM: To observe the effects of electroacupuncture (EA) combined with Compound Salviae Miltiorrhizae (CSM) on the expressions of brain derived neurotrophic factor (BDNF),Tyrosine kinaseB(TrkB) and vascular endothelial growth factor (VEGF) in cortex of chronic cerebral ischemia rats,and to explore its mechanism of treating chronic cerebral ischemia.METHODS: Sprague-Dawley rats were subjected to permanent bilateral Ligation of common carotid artery to prepare chronic cerebral ischemia model rats. The 32 rats were successfully made and were randomly divided into model group,CSM group,EA group,and EA+CSM group(n=8). One week after modeling,CSM at 0.75 g/kg was given to CSM group rats by gastrogavage,EA at Baihui and Dazhui was performed to EA group rats for 30 min,the EA+CSM group was treated with both CSM gastrogavage and electroacupuncture therapy,once a day for 5 weeks. In the experiments, eight SD rats were used as control group.The expressions of BDNF,TrkB and VEGF in the cortex area were detected by immuno-histochemical assay and image analysis.RESULTS: Compared with the normal control group,the expressions of BDNF,TrkB and VEGF in the cortex of the model group were obviously lower (P<0.01 or P<0.05). Compared with the model group,the expressions of BDNF,TrkB and VEGF in the cortex of the CSM group,the EA group,and the EA+CSM group were obviously increased (P<0.01 or P<0.05). The BDNF,TrkB and VEGF expressions in the cortex of EA+CSM group was obviously higher than those of the CSM group and the EA group (P<0.01 or P<0.05).CONCLUSION: EA combined with CSM can protect the chronic cerebral ischemia rats from the ischemia injury,the mechanism of which may related to up-regulating the expressions of BDNF,TrkB and VEGF.

Key words: Compound salviae miltiorrhizae, Electroacupuncture, Cerebral ischemia, BDNF, TrkB, VEGF

中图分类号:

R965.2

张业贵, 丁艳霞, 侯良芹. 针药结合对慢性脑缺血大鼠大脑皮质BDNF、TrkB及VEGF表达的影响[J]. 中国临床药理学与治疗学, 2013, 18(4): 376-381.

ZHANG Ye-gui, DIN Yan-xia, HOU Liang-qin. Effects of electroacupuncture combined with compound salviae miltiorrhizae on the expressions of BDNF, TrkB and VEGF in cortex of chronic cerebral ischemia rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2013, 18(4): 376-381.

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