转化生子因子β1对培养心脏成纤维细胞α-SMA及Cx43表达的影响

中国临床药理学与治疗学 ›› 2014, Vol. 19 ›› Issue (7): 738-742.

转化生子因子β₁对培养心脏成纤维细胞α-SMA及Cx43表达的影响

王新¹, 王德国¹, 赵春梅¹, 邢文¹, 王安才¹, 朱红军², 孙贤林²

¹ 皖南医学院附属弋矶山医院老年科,芜湖 241001,安徽;
² 安徽省立医院心血管内科,合肥 230001,安徽

收稿日期:2014-06-11 修回日期:2014-07-04 发布日期:2014-07-21
通讯作者: 王德国,男,博士, 副主任/副教授,研究方向:心律失常基础与临床研究。 Tel: 0553?5739308 　E?mail: wangdeguo@medmail.com.cn
作者简介:王新,女,副主任医师,研究方向:心血管疾病基础与临床。 Tel: 13855362589 E-mail: wangx308@163.com
基金资助:
国家自然基金(81200142); 省自然科学基金(1208085QH156, 1208085MH129); 医院人才引进科研基金(YR201001)

The effects of TGFβ₁ on the expression of α-SMA and Cx43 in cultured cardiac fibroblasts

WANG Xin¹, WANG De-guo¹, ZHAO Chun-mei¹, XING Wen¹, WANG An-cai¹, ZHU Hong-jun², SUN Xian-lin²

¹ Department of Gerontology, Yijishan Hospital of Wannan Medical College, Wuhu 241001, Anhui, China;
² Department of Cardiology, Anhui Provincial Hospital , Hefei 231000, Anhui, China

Received:2014-06-11 Revised:2014-07-04 Published:2014-07-21

摘要/Abstract

摘要： 目的本研究拟观察转化生子因子β₁(TGFβ₁)诱导乳鼠CFs向MFs分化中α-SMA及Cx43表达的影响。方法酶解-差速贴壁分离法分离培养乳鼠CFs,将 10 ng/mL 的TGFβ₁诱导培养第1天细胞;细胞免疫荧光及共聚焦纤维观察α-SMA及Cx43表达;以免疫印迹和RT-PCR分别半定量比较两者α-SMA及Cx43蛋白和mRNA表达。结果分离培养 2 d 的原代CFs细胞极少表达α-SMA及Cx43蛋白及其mRNA;经TGFβ₁诱导 24 h 后α-SMA及Cx43蛋白及其mRNA表达显著增高(P<0.01)。结论 TGFβ₁可诱导乳鼠CFs向MFs分化,导致α-SMA及Cx43蛋白及其mRNA表达显著增高。

关键词: 成纤维细胞, 肌成纤维细胞, α-平滑肌肌动蛋白;, 缝隙连接蛋白43, 转化生子因子β₁;

Abstract: AIM: To observe the effects of transforming growth factor beta-1(TGFβ₁)on the expression of α-smooth muscle actin(α-SMA) and connexin43 (Cx43) in cultured cardiac fibroblasts (CFs). METHODS: CFs were isolated from neonatal rat by trypsin dissociation and differential adhension. Primary cultures of CFs for one day were subjected to TGFβ₁ (10 ng/mL) incubation for 24 hours. The expressions of α-SMA and Cx43 were determined by immunofluorescence staining. Semiquantitative analysis of mRNA and protein for α-SMA and Cx43 was finished by RT-PCR (reverse transcription polymerase Chain reaction) and Western blots. RESULTS:Little expression of protein and mRNA levels for α-SMA and Cx43 were observed in cultured 2 days primary CFs. TGFβ₁ stimulation for 24 hours led to significantly increased protein and mRNA expression of α-SMA and Cx43.CONCLUSION: Transient TGFβ₁ stimulation causes differentiation of CFs into MFs and up-regulations protein and mRNA expression of α-SMA and Cx43.

Key words: cardiac fibroblasts (CFs), myofibroblasts (MFs), α-smooth muscle actin(α-SMA);, connexin43 (Cx43), transforming growth factor beta-1(TGFβ₁);

中图分类号:

R965.2

王新, 王德国, 赵春梅, 邢文, 王安才, 朱红军, 孙贤林. 转化生子因子β₁对培养心脏成纤维细胞α-SMA及Cx43表达的影响[J]. 中国临床药理学与治疗学, 2014, 19(7): 738-742.

WANG Xin, WANG De-guo, ZHAO Chun-mei, XING Wen, WANG An-cai, ZHU Hong-jun, SUN Xian-lin. The effects of TGFβ₁ on the expression of α-SMA and Cx43 in cultured cardiac fibroblasts[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2014, 19(7): 738-742.

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转化生子因子β₁对培养心脏成纤维细胞α-SMA及Cx43表达的影响

The effects of TGFβ₁ on the expression of α-SMA and Cx43 in cultured cardiac fibroblasts

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