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中国临床药理学与治疗学 ›› 2017, Vol. 22 ›› Issue (11): 1221-1226.

• 基础研究 • 上一篇    下一篇

地塞米松对人胎盘葡萄糖转运功能的作用及机制

商宏恺,傅爱萍,陈露芳,仝进毅,斯 奇   

  1. 浙江省杭州市第一人民医院,南京医科大学附属杭州医院,妇产科,杭州 310006,浙江
  • 收稿日期:2017-04-05 修回日期:2017-07-03 出版日期:2017-11-26 发布日期:2017-12-11
  • 通讯作者: 斯奇,女,本科,研究方向:围产儿医学。 Tel:15990032799 E-mail:806520681@qq.com
  • 作者简介:商宏恺,男,博士,主治医师,研究方向:围产儿医学,妇科肿瘤。 Tel:15990032799
  • 基金资助:

    浙江省自然科学基金(LQ15H040002);杭州市科学发展计划项目(20140733Q06)

Effect and mechanism of dexamethasone on the function of  placental transport of glucose

SHANG Hongkai, FU Aiping, CHEN Lufang, TONG Jinyi, SI Qi   

  1. Department of Obstetrics and Gynecology, Hangzhou First People's Hospital,Hangzhou 310006, Zhejiang, China
  • Received:2017-04-05 Revised:2017-07-03 Online:2017-11-26 Published:2017-12-11

摘要:

目的: 探讨地塞米松对人胎盘葡萄糖转运功能的作用及机制。方法: 取本院治疗并分娩的早产孕妇160例,随机分为对照组和地塞米松组,各80例。随机选取两组各10例胎盘组织样品,采用免疫组织化学检测胎盘生乳素(human placental lactogen,HPL)蛋白。选取人绒毛膜癌细胞系JEG-3细胞株纳入机制研究,根据转染载体的不同分为4组,pcDNA3.1组:JEG-3细胞转染对照载体pcDNA3.1;pcDNA3.1-GRα组:JEG-3细胞转染GRα过表达载体pcDNA3.1-GRα;Control siRNA组:JEG-3细胞转染Control siRNA;GRα siRNA组:JEG-3细胞转染GRα siRNA。分别利用CCK-8法检测胎盘细胞增殖、TUNEL法检测细胞凋亡程度、检测线粒体膜电位、检测胎盘细胞葡萄糖摄取量、荧光分子探针测定胎盘细胞活性氧(reactive oxygen species, ROS)的含量及实时荧光定量PCR(quantitative real-time polymerase chain reaction)检测糖皮质激素受体α(glucocorticoid receptor-α,GRα)、葡萄糖转运蛋白(glucose transporter,GLUT)的相对表达量。结果: 对照组的HPL强阳性细胞多于地塞米松组。与pcDNA3.1组比较,pcDNA3.1-GRα组的细胞增殖能力、线粒体膜电位水平、葡萄糖摄取量显著升高(P<0.05,P<0.01);细胞凋亡、ROS含量明显降低(P<0.01);GRα、GLUT1、GLUT3的相对表达量显著上调(P<0.01)。与Control siRNA组比较,GRα siRNA组细胞的增殖能力、线粒体膜电位水平、葡萄糖摄取量明显下降(P<0.05, P<0.01);细胞凋亡、ROS含量明显升高(P<0.01);GRα、GLUT1、GLUT3的相对表达量均显著下调(P<0.05)。 结论: 地塞米松能够抑制胎盘对葡萄糖的转运功能,并且极有可能通过ROS/AMPK路径实现这一调控。

关键词: 地塞米松, 葡萄糖, 糖皮质激素受体, 葡萄糖转运蛋白, 活性氧

Abstract:

AIM: To explore the effect and mechanism of dexamethasone (DEX) on the function of placental transport of glucose. METHODS: One hundred and sixty premature pregnant women who received treatment and delivery in Hangzhou First People's Hospital were randomly divided into control group and DEX group (n=80). Ten placenta tissue samples were randomly selected from two groups, respectively. Immunohistochemistry was used to detect human placental lactogen. JEG-3 cell line used to research mechanism was divided into 4 groups according to different transfer vectors, i.e group pcDNA3.1, group pcDNA3.1-GRα, group Control siRNA and group GRα siRNA. The CCK-8 method was used to detect placental cell proliferation. TUNEL method was used to detect degree of apoptosis. Mitochondrial membrane potential (MMP) and glucose uptake were detected. Fluorescent probe was used to detect content of reactive oxygen species. Quantitative real-time polymerase chain reaction was used to detect the relative the expression level of GRα and GLUT.RESULTS:The strong expression of HPL in control group was more than that in DEX group. Compared with pcDNA3.1 group, cell proliferation, MMP level and glucose uptake increased significantly in pcDNA3.1-GRα group (P<0.05, P<0.01); yet cell apoptosis and reactive oxygen species (ROS) level decreased significantly (P<0.01) and the relative expression level of GRα, GLUT1 and GLUT3 increased significantly (P<0.01). Compared with control siRNA group, cell proliferation, MMP level and glucose uptake of GRα siRNA group decreased significantly (P<0.05, P<0.01); yet cell apoptosis and ROS level increased significantly (P<0.01) and the relative expression level of GRα, GLUT1 and GLUT3 decreased significantly (P<0.05). CONCLUSION: DEX can induce glucose transport function of placenta, which is likely related with ROS/AMPK pathway.

Key words: dexamethasone, glucose, glucocorticoid receptor, glucose transporter, reactive oxygen species

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