关键词: B3型柯萨奇病毒, 糖尿病, LncRNA, 转录因子

Abstract:

AIM: To explore the specific mechanism of diabetes mellitus induced by Coxsackievirus B3.  METHODS: Human islet cells HUM-CELL-0058 were infected with Coxsackievirus B3. Total RNA was extracted 48 hours after infection and LncRNA related to diabetes mellitus was detected by fluorescence quantitative PCR. Real-time quantitative PCR and Western blot were used to monitor the transcription factors and proteins regulating the transcription of three LncRNA. The over-expression plasmids of PAX6 and NF-kappa B were constructed and transfected into B cells infected with the virus to detect the abnormal expression of LncRNA after coxsackievirus infection. RESULTS:Diabetes-related LncRNA, Lnc-P12792 and MALT1 were up-regulated, while HI-Lnc45 was down-regulated. Real-time quantitative PCR and Western blot results showed that the expression of PAX6, a transcription factor regulating HI-Lnc45 expression, decreased while the expression of NF-kappa B, a transcription factor regulating Lnc-P12792 and MALT1, increased, which was consistent with the trend of transcription factors regulated by PAX6, indicating that the expression difference of LncRNA was due to the abnormal expression of transcription factors of the gene. Real-time quantitative results showed that the three abnormal expression levels of LncRNA returned to the normal level before coxsackievirus B3 infection, which proved that this trend could be saved by regulating the expression of these two transcription factors. CONCLUSION: Coxsackievirus B3 infection of human pancreatic islet β cells can induce up-regulation of transcription factor NF-kappa B and down-regulation of PAX6, and induce abnormal expression of LncRNA related to diabetes mellitus downstream, which may be one of the pathological mechanisms of Coxsackievirus B3 induced diabetes mellitus.

Key words: type B3 Coxsackie virus, diabetes, LncRNA, transcription factor