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中国临床药理学与治疗学 ›› 2004, Vol. 9 ›› Issue (3): 322-325.

• 研究原著 • 上一篇    下一篇

4 种归肝经明目中药对晶状体上皮细胞凋亡相关基因Bcl-2 和Bax 的调控

黄秀榕, 祁明信, 汪朝阳, 王勇   

  1. 福建中医学院病理生理研究中心, 福州350003, 福建
  • 收稿日期:2003-08-14 修回日期:2003-12-20 出版日期:2004-03-26 发布日期:2020-11-21
  • 通讯作者: 黄秀榕, 女, 教授, 博士生导师, 研究方向:白内障临床与基础研究。Tel:0591-3570887  E-mail:qihuang@netease.com
  • 基金资助:
    福建省科技厅重点课题(№98Z173), 福建省卫生厅重点课题(№2000W601)

Effects of four Chinese herbs which pass through liver-channel on expression of Bcl-2 and Bax in rat lens epithelial cells

HUANG Xiu-Rong, QI Ming-Xin, WANG Zhao-Yang, WANG Yong   

  1. Fujian College of Traditional Chinese Medicine, Fuzhou 350003, Fujian, China
  • Received:2003-08-14 Revised:2003-12-20 Online:2004-03-26 Published:2020-11-21

摘要: 目的:探讨4 种归肝经明目中药车前子、青葙子、菊花和熟地对实验性氧化损伤大鼠晶状体上皮细胞(lens epithelial cell, LEC)凋亡相关基因Bcl-2和Bax 的调控。方法:将SD 大鼠双眼随机分成7组:空白对照组、过氧化氢(H2O2)组、吡诺克辛(PS)组、车前子组、青葙子组、菊花组和熟地组。无菌操作摘除眼球并在手术显微镜下分离晶状体, 使晶状体孵育在300 μmol·L-1 H2O2 培养液中复制LEC 凋亡模型, 同时采用4 种归肝经明目中药干预, 置二氧化碳培养箱共同孵育24 h 。取晶状体前囊膜采用免疫组化法检测凋亡相关基因Bcl-2 和Bax 的蛋白表达并进行比较。结果:正常SD 大鼠LEC 中Bcl-2 和Bax 均有表达, Bcl-2 表达较Bax 表达强;H2O2组Bcl-2 表达显著下调, Bax 表达显著上调(P <0.01),Bcl-2/Bax 比率下降;与H2O2 组比较, 4 种归肝经明目中药可明显上调Bcl-2 表达, 下调Bax 表达(P <0.01),Bcl-2/Bax 比率上升;PS 与4 种归肝经明目中药的调节作用相似, 但弱于4 种归肝经明目中药的作用(P <0.05)。结论:4 种归肝经明目中药调控凋亡相关基因Bcl-2 和Bax 的表达可能是其抑制LEC 凋亡的分子机制。

关键词: 中药, 车前子, 青葙子, 菊花, 熟地, 晶状体上皮细胞, 凋亡, 基因

Abstract: AIM: To investigate the effects of four Chinese herbs which pass through liver-channel and improve eyesight, i.e.Semem plantaginis (SP), Semem celosiae (SC), Flos chrysanthemi (FC)and Radix rehmanniae preparata (RRP)on Bcl-2 and Bax, apoptosisrelated genes, in rat lens epithelial cell (LEC)injured by oxidation.METHODS: Eyes of SD rats were divided randomly into 7 groups:control group, hydrogen peroxide group (H2O2), pirenoxine sodium group (PS)and four Chinese herbs (SP, SC, FC and RRP)groups.Eyes were extracted and lenses were separated under operating microscope and sterilized condition.Lenses were incubated in CO2 incubator for 24 h with 300 μmol·L -1 H2O2 and with orwithout four Chinese herbs.The expression of Bcl-2 and Bax protein of LEC were measured and compared by tearing the LEC anterior capsule via immunohistochemical analysis.RESULTS: Both Bcl-2 and Bax expressed in normal lenses of SD rats, and the expression of Bcl-2 was stronger than Bax.In H2O2 group, Bcl-2 expression decreased and Bax expression increased remarkably (P <0.01), and Bcl-2/Bax reduced.In four Chinese herbs groups, there were up-regulation in Bcl-2 expression and down-regulation in Bax expression, and Bcl-2/Bax increased compared with H2O2 group (P <0.01). The changes in PS group were similar to four Chinese herbs groups, but much weaker than those in four Chinese herbs groups (P <0.05).CONCLUSION: Four Chinese herbs regulate the expression of apoptosis-related genes,Bcl-2 and Bax,which may be the molecular mechanism of LEC apoptosis inhibition by four Chinese herbs.

Key words: Chinese herbs, Semem plantaginis, Semem celosiae, Flos chrysanthemi, Radix rehmanniae preparata, lens epithelial cell, apoptosis, genes

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