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中国临床药理学与治疗学 ›› 2003, Vol. 8 ›› Issue (1): 98-100.

• 研究原著 • 上一篇    下一篇

大黄中有效成分提取分离条件的优化

肖艳, 杜智敏1   

  1. 大庆油田总医院药剂科, 大庆 163001, 黑龙江; 1哈尔滨医科大学第二附属医院临床药理室, 哈尔滨 151000, 黑龙江
  • 收稿日期:2002-08-22 修回日期:2002-09-22 发布日期:2020-11-25
  • 通讯作者: 肖艳, 女, 硕士, 副主任药师。Tel:0459-5805271 E-mail:xy@zyy.dq.cnpc.com.cn

Optimization of the extraction and isolation for anthraquinone component from rhubarb

XIAO Yan, DU Zhi-Ming1   

  1. Department of Pharmacy, Daqing Oil Field General Hospital, Daqing 163001, Heilongjiang; 1Department of Clinical Pharmacology, the Second Affiliated Hospital, Harbin Medical University, Harbin 151000, Heilongjiang
  • Received:2002-08-22 Revised:2002-09-22 Published:2020-11-25

摘要: 目的: 优化从大黄中提取分离大黄蒽醌类有效成分的条件, 以提高其收率和纯度。方法: 用乙醇回流提取大黄4 次, 得到粗提取液后, 用硼酸盐缓冲溶液萃取, 萃取液经高压制备色谱分离纯化, 得大黄酸、大黄素、大黄酚单体成分, 丙酮重结晶后得纯品。色谱柱为YWG C18柱(4.6 mm ×250 mm, 10 μm) 流动相为甲醇∶水∶高氯酸(9:1:0.01), 流速1.0 ml·min-1, 检测波长254 nm。结果: 用高压制备系统可分离纯化大黄蒽醌类有效成分, 得到大黄酸、大黄素、大黄酚单体成分, 各成分纯度在80 %以上, 大黄素收率达0.6 %。结论: 醇提法提取大黄蒽醌类有效成分的方法简单, 提取率高。高压制备系统可分离纯化大黄蒽醌类有效成分, 得单体纯品, 且收率高纯度好。

关键词: 大黄, 蒽醌成分, 提取, 分离

Abstract: AIM: To optimize the method of extracion and isolation for anthraquinone component from rhubarb for increasing the extraction rate and purity.METHODS: Rhubarb was extracted 4 times in the refluxing method with ethanol.The borate buffer solution was used as extracted fluid.Rhein, rheum emodin and chrysophanol could be obtained by Deltar 4 000 high-pressure making system, and the pure product obtained by recrystallizing with acetone.The YWG C18 column was (4.6 × 250) mm, the flow rate 1.0 ml.min-1, and UV detection wavelength 254 nm.Methanol, water and acid perchloride were taken as mobile phase with the ratio of 9:1:0.01.RESULTS: Deltar 4000 high-pressure making system could be used to isolate the anthraquinone component from rhubarb, the purity of rhein, rheum emodin and chrysophanol was more than 80 %, and the ertraction rate of rheum emodin reached 0.6 %.CONCLUSION: The extraction of the anthraquinone component is simple and cheap with ethanol from rhubarb, and the extraction rate is high.Deltar 4000 high pressure making system can be used to isolate the anthraquinone component from rhubarb with a high the extraction rate and purity.

Key words: rhubarb, anthraquinone component, extraction, isolation

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