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中国临床药理学与治疗学 ›› 1999, Vol. 4 ›› Issue (2): 104-107.

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羟丁酸钠和氯胺酮对心肌细胞毒性作用的对比研究

张铁铮, 王凤学, 罗军, 陈伟   

  1. 沈阳军区总医院, 沈阳 110015
  • 收稿日期:1999-04-11 出版日期:1999-06-26 发布日期:2020-12-04
  • 作者简介:张铁铮,男,37岁,硕士研究生,主治医师,主要从事临床麻醉和麻醉基础与临床研究。

Cardiotoxicity of sodium γ-hydroxybutyrate and ketamine in neonatal primary myocardial cell cultures

ZHANG Tie-Zheng, WANG Feng-Xue, LUO Jun, CHEN Wei   

  1. Department of Anaesthesiology, The General Hospital of Shenyang Command, PLA, Shenyang 110015
  • Received:1999-04-11 Online:1999-06-26 Published:2020-12-04

摘要: 目的 对比研究羟丁酸钠和氯胺酮对原代培养大鼠心肌细胞的毒性作用。方法 将经原代培养成活4d 后的大鼠心肌细胞分为5 组, 每组6 孔。包括对照组, 小剂量(HL, 3×10-3mol·L-1)与大剂量(HH, 3×10-2 mol·L-1)羟丁酸钠组和小剂量(KL, 1×10-5 mol·L-1)与大剂量(KH, 1×10-4 mo l·L-1)氯胺酮组。各组均于实验开始后8 h 终止反应, 评定心肌细胞搏动功能、观察细胞形态学改变、测定心肌细胞酶及电解质变化。结果 与对照组相比, KL 组心肌细胞搏动频率明显加快(P<0.05), 而KH 组减慢(P<0.05)。细胞形态学亦有相应变化。KH 组LDH 和AST 释放量增加(P<0.05), ALP 活性下降(P<0.05)。而HL、HH 组的上述指标均无明显变化。各组间电解质变化未见显著差异。结论 高浓度的氯胺酮具有直接的心肌抑制作用, 而低浓度的氯胺酮却有正性变时性和变力性作用。无论低浓度或高浓度的羟丁酸钠, 均未见心肌细胞毒性作用。

关键词: 羟丁酸钠, 氯胺酮, 心肌, 细胞毒性, 细胞培养

Abstract: Aim The cardio to xicity profile of sodium γ-hydroxy butyrate and ketamine was investigated.Methods 4 day-old cultured neonatal primary my ocardial cells obtained from 2to3-day-old Wistar rats were divided into 5 groups, with group C serving as control and groups HL, HH, KL and KH treated with sodium γ-hydroxy butyrate (3×10-3 mol·L-1 and 3×10-2 mol·L-1) and ketamine (1×10-5 mol·L-1 and 1×10-4 mo l·L-1)for 8 h. The contractility、morphology and cytoplasmic enzyme (LDH,AST, CK and ALP)release of my ocardial cell and the concentration of elect rolytes (K+, Na+, Cl-and Ca2+)in the medium were measured 8 h following the intrav enous anesthetics administ ration.Results In group KL, the beatingrate of neonatal my ocardial cell was increased (P<0.05), while in group KH decreased significantly (P<0.05).The release of LDH and AST in the cell cultures of group KH was increased and ALP decreased (P<0.05)associated with an adve rse effect on morphology.The results show that group HL, HH and KL had little or no effect on all indices of toxicity evaluated electrolytes were not significantly different between groups. Conclusion The high concent rations of ketamine has significant direct myocardial depressant action, while sodium γ-hydroxy butyrate and low concent rations of ketamine have little or no effect on cardio to xity, in fact, the low concent rations of ketamine has positive chronot ropic and inot ropic action.

Key words: sodium γ-hydroxybutyrate, ketamine, my ocardium, cy to toxicity, cell culture

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