Effects of platelet activation on renal tubular epithelial cells in sepsis

doi:10.12092/j.issn.1009-2501.2018.03.007

Abstract

Abstract:

AIM: To investigate the effect of platelet activation on renal tubular epithelial cells in sepsis. METHODS: ELISA was used to detect the inflammatory cytokines IL-1β and IL-6 secreted by platelets. Renal tubular epithelial cells and platelets were co-cultured in the co-culture system. Western blot was used to test p65 and p-p65 protein in renal tubular epithelial cells in the lower chamber. IL-1β and IL-6 mRNA expression level was measured by qRT-PCR. Cecal ligation and puncture(CLP) were used to construct the sepsis model, and TLR4 inhibitor TAK-242 was injected in caudal vein. In order to observe injury degree of renal tissue, EdU experiment and serum creatinine detection were carried out. RESULTS: IL-1β and IL-6 cytokines secreted by active platelets were increased in the presence of different concentrations of LPS. In the platelet and renal tubular epithelial cells co-culture system, p-p65 protein was increased in the tubular epithelial cells. TAK-242 could inhibit platelet activation, and reduce the renal tubular epithelial cell inflammatory cytokines secretion.The proliferation and pathological status were improved, and serum creatinine was decreased. CONCLUSION: The platelets in septic patients are aggregated and activated. inflammatory cytokines are increased. These cytokines activate NF-κB signaling pathway of renal tubular epithelial cells, leading to renal injury. TLR4 inhibitors have potential clinical value to improve the renal injury in sepsis.

Key words: sepsis, kidney injury, platelet activation, NF-κB signaling pathway

CLC Number:

R965.2

FANG Jingjing, TAO Jing, HUANG Qin, SHI Chaolu, YAN Biqing, GAI Lei, LI Xuguang. Effects of platelet activation on renal tubular epithelial cells in sepsis[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(3): 283-289.

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