iTRAQ technology based differences of protein expression in sepsis-associated encephalopathy

doi:10.12092/j.issn.1009-2501.2019.11.009

Abstract

Abstract:

AIM: To analyze the differential protein expression in brain tissue of septic rats by isobaric tags for relative and absolute quantification(iTRAQ) technology. METHODS: Thirty male Wistar rats were randomly divided into sepsis group and control group, with 15 rats in each group. The brain electrical monitoring electrode was placed 3 days before modeling, and the rat sepsis model was prepared by cecal ligation and puncture (CLP). After 48 hours of modeling, the protein was extracted, iTRAQ labeled, the differential protein was screened, and the difference was analyzed. The relationship between protein and sepsis was also analyzed. RESULTS:Compared with the control group, 91 proteins with differential expression were screened from the brain tissue of the sepsis group. Among them, 68 were up-regulated and 23 were down-regulated. It was related to the related gene functions such as stress response, cell signal transduction, glucocorticoid receptor, immune response, insulin-like growth factor, and cellular material energy metabolism. CONCLUSION: A series of abnormal protein expressions in brain tissue of septic rats can be quickly analyzed by iTRAQ technology.

Key words: sepsis, brain, iTRAQ

CLC Number:

R965.2

QIAO Yang, CHEN Qun, YANG Guang,CAO Yingya, LU Weihua. iTRAQ technology based differences of protein expression in sepsis-associated encephalopathy[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1269-1274.

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