Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body

doi:10.12092/j.issn.1009-2501.2018.04.008

Abstract

Abstract:

AIM: To study the mechanism of Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body. METHODS: The Parkinson model mice was constructed by 6-OHDA injection, and the mice model was successfully constructed by TH immunohistochemical staining and behavior intervention of Antrodia camphorata polysaccharide, neurobehavioral experiments were performed at four time points. The independent behavioral ability and coordination ability of mice were detected by rotating rod test and climbing rod test respectively. The peripheral blood of the tail vein of mice was collected at four time points and the expression of Caspase1 and IL-1β in peripheral blood was detected by ELISA method. After the 7-day intervention, the mice were killed and the brain tissue striatum was taken. Caspase1, pro Caspase1 and NLRP3 in striatum were detected by Western blot; monoamine neurotransmitters in striatum were determined by high performance liquid chromatography; mRNA expression of Caspase1, NLRP3, IL-1β, IL-4 and IL-6 were detected by RT-QPCR. NISSl staining was used to detect the apoptosis of neural cells in the brain tissue of mice. RESULTS: 6-OHDA injection could cause Parkinson like lesions in mice, and the expression of TH protein was down regulated significantly. The behavior of mice was significantly improved after Antrodia camphorata polysaccharide intervention（P<0.05）; in striatum, the expression of Caspase1, proCaspase1, NLRP3 were significantly lower than those in model mice（P<0.05）; the mRNA expression of caspase1, NLRP3, IL-1 beta, IL-4 and IL-6 was down regulated（P<0.05）; the expression of monoamine neurotransmitters increased in striatum（P<0.05）. CONCLUSION: Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body. This may be one of the mechanisms for the treatment of Parkinson with Antrodia camphorata polysaccharide.

Key words: Antrodia camphorata polysaccharide, NLRP3-Caspase1 inflammatory body, Parkinson mice, neurobehavioral

CLC Number:

R965.2

YANG Yi，GUAN Qiaobin，GUO Li，HAN Chenyang. Antrodia camphorata polysaccharide improved neurobehavioral function in Parkinson mice via reducing the expression of NLRP3-Caspase1 inflammatory body[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(4): 406-412.

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