Protective effects of β-asarone on PC12 cells injury induced by Aβ1-42 activated ACM

doi:10.12092/j.issn.1009-2501.2018.06.002

Abstract

Abstract:

AIM: To investigate the effect of different concentrations of Aβ _1-42 activated-ACM cell on the viability and expression of BDNF, AChE of PC12 cells, and to explore the protective effects of β-asarone on the PC12 cells. METHODS: The logarithmic growth PC12 cells were randomly divided into normal group, ACM (Aβ_1-42 3.3 μmol/L) group, ACM (Aβ_1-42 10 μmol/L) group and ACM (Aβ_1-42 30 μmol/L) group. The Real time cell analysis (RTCA) system and MTT assay were used to detect the survival rate of PC12 cells, respectively; Western blot assay was used to detect the expression of BDNF, AChE. The logarithmic growth PC12 cells divided into normal group, ACM (Aβ_1-42 μmol/L) group, β-asarone(18.5 μg/mL, 55.5 μg/mL, 166.7 μg/mL) group randomly. The change of survival rate of PC12 cells was detected by RTCA assay; the expression of BDNF, AChE were detected by Western blot. RESULTS: There was no significant difference in the survival rate of PC12 cells in each group after ACM activated for 24 h (P>0.05); while the survival rate of PC12 cells was significantly reduced in ACM (Aβ_1-42 10 μmol/L) group and ACM (Aβ_1-42 30 μmol/L) group (P<0.01) after ACM activated for 36 h; the expression of BDNF, AChE protein increased significantly with the increase of the concentration of Aβ_1-42 (P<0.05 or P<0.01); the survival rate of PC12 cells were significantly increased with β-asarone (18.5 μg/mL, 55.5 μg/mL, 166.7 μg/mL); the expression of AChE in β-asarone (55.5 μg/mL, 166.7 μg/mL) group was significantly lower than that in the model group (P<0.05 or P<0.01); the expression of BDNF in β-asarone (55.5 μg/mL) group was increased compared with that of the model group (P<0.05). CONCLUSION: β-asarone can inhibit the increase of AChE expression, and promote the expression of BDNF, suggesting that β-asarone has a certain protective effect on neurons.

Key words: β-asarone, Aβ_1-42, ACM, PC12 cells, BDNF, AChE

CLC Number:

R965.2

HE Yin, BAO Yuting, YAO Ying, XUAN Ling, YANG Yuanxiao. Protective effects of β-asarone on PC12 cells injury induced by Aβ_1-42 activated ACM[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(6): 608-613.

References

［1］Carter SF, Scholl M, Almkvist O, et al. Evidence for astrocytosis in prodromal Alzheimer disease provided by 11CdeuteriumLdeprenyl: a multitracer PET paradigm combining 11Cpittsburgh compound B and 18FFDG［J］. J Nucl Med, 2012, 53(1): 37-46.
［2］Hoozemans JJ, Rozemuller AJ, van Haastert ES, et al. Neuroinflammation in Alzheimer' s disease wanes with age［J］. J Neuroinflammation，2011, 8: 171.
［3］Li CC, Xing GH, Dong MX, et al. Betaasarone protection againse betaamyloid induced neurotoxicity in PC12 cells via JNK signaling and modulation of Bcl2 family proteins［J］. Eur J Pharmacol, 2010, 635(1/2/3): 96.
［4］Zou DJ, Wang G, Liu JC, et al. Betaasarone attenuates betaamyloid induced apoptosis through the inhibition of the activation of apoptosis signalregulating kinase 1 in SHSY5Y cells［J］. Pharmazie, 2011, 66(1): 44.
［5］何潆, 何嘉娜, 付俊, 等. β细辛醚对Aβ142诱导星形胶质细胞活化所致PC12细胞损伤的保护作用研究［J］. 中国中药杂志, 2016, 41(7): 1282-1288.
［6］Yang YX, Chen YT, Zhou XJ, et al. Betaasarone, a major component of Acorus tatarinowii Schott, attenuates focal cerebral ischemia induced by middle cerebral artery occlusion in rats［J］. BMC Complement Altern Med, 2013, 13(236): 1-6.
［7］Querfurth HW, LaFerla FM. Alzheimer's disease［J］. N Engl J Med，2010, 362(4): 329-344.
［8］Kokubo H, Kayed R, Glabe CG, et al. Soluble Abeta oligomers ultrastructurally localize to cell processes and might be related to synaptic dysfunction in Alzheimer's disease brain［J］. Brain Res, 2005, 1031(2):222-228.
［9］Walsh DM, Klyubin I, Fadeeva JV, et al. Amyloidbeta oligomers: their production, toxicity and therapeutic inhibition［J］. Biochem Soc Trans, 2002, 30(4):552-557.
［10］陈奕芝, 王绮雯, 梁毅, 等. β细辛醚对谷氨酸所致脑皮层神经元损伤的保护作用［J］. 中药材, 2007, 30(4): 436-440.
［11］Geng Y, Li C, Liu J,et al.Betaasarone improves cognitive function by suppressing neuronal apoptosis in the betaamyloid hippocampus injection rats［J］. Biol Pharm Bull, 2010, 33(5): 836-843.
［12］Liu L, Fang YQ, Xue ZF, et al. Betaasarone attenuates ischemiareperfusioninduced autophagy in rat brains via modulating JNK, pJNK, Bcl2 and Beclin 1［J］. Eur J Pharmacol, 2012, 680(1/2/3):34-40.
［13］吴洁, 吴红海, 于洋. 孕酮抑制Aβ 诱导星形胶质细胞活化所致神经元损伤［J］. 中国药理学通报, 2014, 30(11): 1539-1543.
［14］J Wahlert A,Funkelstein L,Fitzsimmons B,et al.Spinal astrocytes produce and secrete dynorphin neuropeptides［J］.Neuropeptides,2013,47(2):109-115.
［15］Jha MK, Seo M, Kim JH, et al. The secretome signature of reactive glial cells and its pathological implications［J］. Biochim Biophys Acta, 2012, 23, 15709639(12)002865.
［16］施晴, 薛锦慧, 刘彦君. 基于 AChE 双位点抑制的新型多靶向配基抗阿尔茨海默病研究进展［J］. 中国临床药理学与治疗学, 2016, 21(8): 943-949.

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Protective effects of β-asarone on PC12 cells injury induced by Aβ_1-42 activated ACM

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