Table of Content

Volume 23 Issue 6
26 June 2018

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Probucol inhibited metastasis and invasion by reversing Warburg effect and regulated reprogramming of hepatoma cell HepG2

WANG Jin, YANG Yi, GUO Li, LI Wenyan, RUAN Shuiliang

2018, 23(6):  601-607.  doi:10.12092/j.issn.1009-2501.2018.06.001

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AIM: To study the mechanism of probucol modulated the metabolic reprogramming of HepG2, reversed the Warburg effect and inhibited its metastasis and invasion, so as to provide support for the research of lipid metabolism regulating drugs. METHODS: HepG2 cells were divided into control group (NC), high dose group (High), middle dose group (Middle) and low dose group (Low) after cultured in vitro. The cells in the high dose, middle dose and low dose group were treated with probucol of 100, 50, 10 μmol/L, respectively. CCK8 was used to detect cell viability; the cell cycle changes were detected by flow cytometry; the cell migration assay was performed by cell scratch test; the cell invasion ability was detected by Transwell experiment; the expression of hexokinase (HK), pyruvate kinase (PK), lactate dehydrogenase (LDH), phospho fructose kinase (PFKP), pyruvate dehydrogenase (PDH) and citric acid synthase (CS) in cells were detected by Western blot; the level of lactic acid in the medium was detected by the kit; the pressure of glycolysis was detected by ECAR; and the oxygen absorption was detected by the 929 oxygen system. RESULTS:Probucol dosedependently inhibited the proliferation of HepG2, and blocked cell cycle. Probucol increased the expression of HK, PK, LDH, PFKP, PDH and CS, increased oxygen consumption and reduced the production of lactic acid, and promoted the cycle of three carboxylic acids. Probucol also inhibited the invasion and migration of HepG2. CONCLUSION: Probucol can regulate the metabolic reprogramming, reverse the Warburg effect of hepatoma cell HepG2，which can inhibit the proliferation, migration and invasion of tumor cells.

Protective effects of β-asarone on PC12 cells injury induced by Aβ_1-42 activated ACM

HE Yin, BAO Yuting, YAO Ying, XUAN Ling, YANG Yuanxiao

2018, 23(6):  608-613.  doi:10.12092/j.issn.1009-2501.2018.06.002

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AIM: To investigate the effect of different concentrations of Aβ _1-42 activated-ACM cell on the viability and expression of BDNF, AChE of PC12 cells, and to explore the protective effects of β-asarone on the PC12 cells. METHODS: The logarithmic growth PC12 cells were randomly divided into normal group, ACM (Aβ_1-42 3.3 μmol/L) group, ACM (Aβ_1-42 10 μmol/L) group and ACM (Aβ_1-42 30 μmol/L) group. The Real time cell analysis (RTCA) system and MTT assay were used to detect the survival rate of PC12 cells, respectively; Western blot assay was used to detect the expression of BDNF, AChE. The logarithmic growth PC12 cells divided into normal group, ACM (Aβ_1-42 μmol/L) group, β-asarone(18.5 μg/mL, 55.5 μg/mL, 166.7 μg/mL) group randomly. The change of survival rate of PC12 cells was detected by RTCA assay; the expression of BDNF, AChE were detected by Western blot. RESULTS: There was no significant difference in the survival rate of PC12 cells in each group after ACM activated for 24 h (P>0.05); while the survival rate of PC12 cells was significantly reduced in ACM (Aβ_1-42 10 μmol/L) group and ACM (Aβ_1-42 30 μmol/L) group (P<0.01) after ACM activated for 36 h; the expression of BDNF, AChE protein increased significantly with the increase of the concentration of Aβ_1-42 (P<0.05 or P<0.01); the survival rate of PC12 cells were significantly increased with β-asarone (18.5 μg/mL, 55.5 μg/mL, 166.7 μg/mL); the expression of AChE in β-asarone (55.5 μg/mL, 166.7 μg/mL) group was significantly lower than that in the model group (P<0.05 or P<0.01); the expression of BDNF in β-asarone (55.5 μg/mL) group was increased compared with that of the model group (P<0.05). CONCLUSION: β-asarone can inhibit the increase of AChE expression, and promote the expression of BDNF, suggesting that β-asarone has a certain protective effect on neurons.

Metformin inhibits p38MAPK signaling pathway and oxidativestress in rat glomerular mesangial cells cultured with high glucose

YAO Xinming, KONG Xiang, ZHAO Yongli, HUA Qiang, HE Chunling, YU Dan, LI Yeqiong, DAI Xiaokang, YE Shandong

2018, 23(6):  614-620.  doi:10.12092/j.issn.1009-2501.2018.06.003

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AIM: To observe relationship between p38MAPK signaling pathway and oxidative stress in rat glomerular mesangial cells (MCs) cultured in high glucose medium and the regulation of metformin. METHODS: Rat glomerular MCs were cultured in a complete medium, and were divided into five groups: normal control group (group NC), high glucose group(group HG) , metformintreated group (group MET), SB203580treated group (group SB), N-acetylcysteinetreated group (group NAC). Intracellular reactive oxygen species (ROS) content of rat glomerular MCs was tested by flow cytometry. Superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in supernatant fluid were detected by colorimetry and ELISA, respectively. P22phox mRNA of rat glomerular MCs was determined by real-time quantitative PCR. P22phox protein and phosphorylation of p38MAPK(p-p38MAPK)protein of rat glomerular MCs were detected by Western blot. RESULTS: Compared with group NC, the activity of SOD was decreased, whereas the level of MDA, intracellular ROS content, P22phox mRNA and protein, p-p38MAPK protein expression were increased in group HG (P<0.05). When metformin was added to the high glucose medium, the activity of SOD was increased, meanwhile,the level of MDA, intracellular ROS content, P22phox mRNA and protein,pp38MAPK protein expression were decreased (P<0.05), and the results were similar with SB203580 or N-acetylcysteine was added to the high glucose medium (P<0.05). CONCLUSION: Metformin can alleviate high glucose induced oxidative stress and p-p38MAPK protein expression of rat glomerular MCs,which maybe contribute to its renoprotection.

Long non-coding RNA TSIX regulates the proliferation of human pancreatic cancer cells by targeting miR-384

SHEN Binbin, ZHOU Jun, LI Liang, LU Ning, YAO Ming

2018, 23(6):  621-626.  doi:10.12092/j.issn.1009-2501.2018.06.004

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AIM: To observe the expression levels of long non-coding RNA (LncRNA-TSIX) in the pancreatic cancer tissues and cell lines, and to explore its effect on cell proliferation and its possible mechanism. METHODS: qPCR was used to detect the expression of TSIX in 5 pancreatic cancer cell lines (BxPC-3, CAPAN-1, AsPC-1, CFPAC-1 and SW1990) and human normal pancreatic ductal epithelial cells HPDE6-C7, and 7 pancreatic cancer tissues with para-cancer tissues were also collected. Bioinformatics prediction and analysis of miRNAs and downstream genes paired with TSIX were performed. SW1990 cells were infected with recombinant lentiviral vector carrying TSIX siRNA gene (LV-siRNA) as experimental group and negative control lentivirus particles (LV-NC) as control group. The effect of TSIX low-expression on miR-384 and Gab2 mRNA expression was detected by qPCR, and the expression of Gab2 and PI3K/AKT pathway related protein was determined by Western blot. The effects of TSIX low-expression on the cell cycle, viability and colony formation ability of SW1990 cells were detected by flow cytometry, MTT assay and colony formation assay. RESULTS:Compared with HPDE6-C7 cells, TSIX was highly expressed in the pancreatic cancer cell lines (P<0.01). The expression of TSIX was the highest in the SW1990 cells (P<0.01). The expression level of TSIX in pancreatic cancer tissues was higher than that in its adjacent tissues (P<0.05). The expression of TSIX in the SW1990 cells transfected with TSIX siRNA was significantly decreased (P<0.01), the expression of miR384 was increased (P<0.01), and the mRNA expression of Gab2 was decreased (P<0.01). Low expression of TSIX significantly inhibited the cell cycle, and inhibited the cell viability and colony formation ability (P<0.01). CONCLUSION: LncRNA TSIX is overexpressed in the pancreatic cancer tissues and cell lines. Knockdown of TSIX expression inhibits the proliferation of SW1990 cells. The possible mechanism is that TSIX regulates the expression of Gab2 gene by combining with miR-384.

Effects of compound Cotinus coggygria oral liquid on liver homogenate and liver tissue of rats

HAO Shaojun, MA Junjie, KONG Xuejun, SU Feng, LI Jun, LI Wenjun, ZHANG Zhengchen

2018, 23(6):  627-631.  doi:10.12092/j.issn.1009-2501.2018.06.005

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AIM: To observe the effects of compound Cotinus coggygria oral liquid on ALT, AST, SOD, MDA, Hyp levels of liver homogenate and liver tissue in rats with chronic liver injury induced by carbon tetrachloride. METHODS: Seventy-two male SD rats, half male and half female, were randomly divided into blank group, model group, Huganing tablet group (0.675 g/kg), high, medium and low dose of compound Cotinus coggygria oral liquid group (15, 10, 5 mL/kg, respectively). Rat model of chronic liver injury was induced by intraperitoneal injection of 50% carbon tetrachloride olive oil solution 1 mL/kg, 2 times/week for 8 weeks, and assigned drugs were administered at fifth weeks at the same time accordingly. Twenty-four hours after the final administration, rats were anesthetized by intraperitoneal injection of chloral hydrate, and the inferior vena cava blood was collected to detect the levels of AST, ALT, MDA, Hyp, and SOD in liver homogenate. Also, a small amount of liver tissue was collected to observe the pathological changes. RESULTS: Rat model of chronic liver injury model was successfully induced. Compared with the model group, high, medium and low dose of compound Cotinus coggygria oral liquid and Huganning tablet could significantly reduce the ALT, AST and MDA level (P<0.01), increase SOD level (P<0.01) , lower Hyp levels (P<0.01, P<0.05) in liver homogenate, and could significantly improve liver pathological damages of model rats (P<0.01). CONCLUSION: Compound Cotinus coggygria oral liquid has a better protective effect on chronic liver injury induced by CCl₄.

Effects of Chinese medicine Yuxia capsule on renal fibrosis in spontaneously hypertensive rats

CHENG Xiaolong, HU Kunmei, HU Haoran，XU Hanghang, YANG Jieren, HAN Jun

2018, 23(6):  632-639.  doi:10.12092/j.issn.1009-2501.2018.06.006

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AIM: To investigate the improvement of Chinese medicine Yuxia capsule on renal fibrosis in spontaneously hypertensive rats (SHR) and its possible mechanism. METHODS: WKYs（Wistar-Kyoto rats）were set as normal control group (0.5% CMC-Na, 5 mL/kg). SHRs were randomly divided into SHR model group (0.5% CMC-Na, 5 mL/kg), Yuxia capsule high dose group (0.6 g/kg), middle dose group (0.3 g/kg) and low dose group (0.15 g/kg). Seven rats in each group. According to the above dose, each group of rats were gavaged once daily for 10 weeks. Diastolic blood pressure (DBP) was measured before administration and 2, 4, 6, 8, 10 weeks during the drug administration by non-invasive tail arterial pressure system. Urine was collected before and 3, 5, 8 week after administration and the urine volume was measured. Automatic biochemical analyzer was utilized to measure the contents of serum creatinine (Scr) and blood urea nitrogen (BUN). Immunohistochemistry was used to detect the levels of TGF-β1 positive cells in kidney. Quantitative reverse transcription PCR (RTqPCR) was applied to detect the expression levels of TGFβ1 mRNA. Western blot was adopted to measure the expressions of ERK1/2，p-ERK1/2，P38，p-P38，MMP-9，TIMP-1 proteins in kidney. RESULTS: Yuxia capsule could dose-dependently reduce DBP and the contents of Scr and BUN, increase urine volume. Expressions of TGF-β1，MMP-9，TIMP-1 and phosphorylation of P38 and ERK1/2 protein level in Yuxia capsule group were lower than those in model group（P<0.05, P<0.01）. CONCLUSION: Traditional Chinese medicine Yuxia capsule demonstrats protective effects against renal fibrosis in a dosedependent manner within a certain range in SHR, of which the mechanism may be associated with reducing diastolic blood pressure, promoting urination, inhibiting P38/MAPK, ERK/MAPK signal pathway and the expressions of TGF-β1，MMP-9 and TIMP-1, recovering the ratio of MMP-9/TIMP-1.

Inhibitory effect and mechanism of Adina rubella flavonoids on platelet aggregation and thrombosis

FANG Qingxia，ZOU Xiaozhou，YU Wenying，FANG Jie，MO Li，YU Chenhuan

2018, 23(6):  640-645.  doi:10.12092/j.issn.1009-2501.2018.06.007

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AIM: To investigate the effect and mechanism of Adina rubella flavonoids (ARF) on platelet aggregation and activation. METHODS: Different stimulus［adenosine 5'diphosphate (ADP)，arachidonic acid (AA), platelet activating factor (PAF)］were used to induce platelet aggregation in vitro. A rat model of acute pulmonary embolism caused by ADP, a mouse model of bleeding time and a rat model of arteriovenous shunt thrombosis were established to observe the inhibitory effect of ARF on platelet aggregation and thrombosis. The expression of CD41 and the activation of PI3K/Akt signal pathway were illustrated by Western blot. RESULTS: Compared with the control group, all ARF groups of the low-dose, middle-dose and high-dose significantly improved the survival rates of acute pulmonary embolism rats, prolonged the bleeding time and inhibited arteriovenous shunt thrombosis formation. ARF also inhibited platelet aggregation, and up-regulated expression of CD41 and the activation of PI3K/Akt signal pathway induced by ADP. CONCLUSION: ARF significantly inhibits the platelet aggregation and thrombosis, the mechanism may be related to CD41/PI3K/Akt signaling pathway.

Nimodipine combined with flunarizine for preventing and treating migraine: A Meta-analysis

SHAO Yaojun, ZHENG Tong,WANG Yanqiu,LIU Ling,YAO Yingshui

2018, 23(6):  646-652.  doi:10.12092/j.issn.1009-2501.2018.06.008

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AIM: To evaluate the therapy efficacy and safety of nimodipine combined with flunarizine for preventing and treating migraine. METHODS: Eligible papers about randomized controlled trials of nimodipine combined with flunarizine for preventing and treating migraine and published between 2000 and 2017 were retrieved from PubMed and from online Chinese periodicals, the full-text databases of Wan Fang, VIP and the Chinese National Knowledge Infrastructure.J adad scale was used to assess the quality of included articles and a meta-analysis was performed by RevMan 5.3 software. RESULTS: A total of ten papers were included finally after assessing the quality of studies. A total of 423 people were in the experimental group, in which 398 cases were effective. The control group was 423,in which 324 cases were effective. Meta-analysis showed that there was a significant difference between experimental group and control group［OR=4.87,95%CI（3.07，7.74）, P＜0.01］. Subgroup analysis was performed based on dose and course of treatment. The result showed that it had no heterogeneity between subgroups and different subgroups all had treatment effect. The difference of adverse effect was statistical between two groups［OR=0.41，95%CI（0.20，0.85），P=0.02］. After treatment, symptoms score evaluation of experimental group was better than the control group (P＜0.01). CONCLUSION: The nimodipine combined with flunarizine showed better efficacy and safety for migraine compared with flunarizine alone.

Effect and safety of lactoferrin for prevention of late-onset sepsis in premature neonates: a systematic review

WEI Liuping, WU Qing, YIN Nange, YANG Bin, MAO Jinxiao

2018, 23(6):  653-660.  doi:10.12092/j.issn.1009-2501.2018.06.009

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AIM: To systematically review the effect and safety of lactoferrin for prevention of late-onset sepsis in premature neonates. METHODS: Cochrane Library databases, Pubmed, EMBase, CNKI, VIP Database, and WHO International Clinical Trial Registry, US Clinical Trial Registration and EU Clinical Trial Register were searched for randomized controlled trials (RCT), in which lactoferrin versus placebo in the prevention of late-onset sepsis in premature infants. After quality evaluation and data extraction，Meta-analysis was performed with Rev Man 5.2 software. RESULTS: A total of 8 RCT (published in ten reports) were included，involving 1 755 patients. Results of Meta analysis indicated that lactoferrin with or without a probiotic decreased late-onset sepsis, fungal sepsis and necrotizing enterocolitis (Bell's stage II or III) in preterm infants with no adverse effects or intolerances, and reduced late-onset sepsis-cause mortality. CONCLUSION: Lactoferrin can effectively prevent the development of late-onset sepsis in premature infants. However, due to the limit of methodological quality and sample size, it remains to be further verified with more large-scale and high-quality RCT. Furthermore, clarification regarding dosing, duration and type of lactoferrin (human or bovine) of prevention of late-onset sepsis for preterm infants is still needed.

Effects of ACE2 polymorphism on the clinical outcomes of irbesartan for the treatment of stage 2 hypertension concomitant with chronic renal failure

MENG Jingqian, ZHAO Yingying, ZHANG Wenji, ZHANG Wanzhe, SHEN Pengxiao，LIU Ding, SU Chenhao, SHEN Jie, WANG Jiansheng

2018, 23(6):  661-666.  doi:10.12092/j.issn.1009-2501.2018.06.010

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AIM: To investigate the effects of ACE2 gene polymorphism on the treatment of irbesartan for the patients with stage-2 hypertension concomitant with chronic renal failure. METHODS: A total of 135 patients with stage-2 hypertension concomitant with chronic renal failure in the department of nephrology and rheumatism were included in this study. All of the patients were given irbesartan 150-300 mg once daily. After 4 weeks treatment, the antihypertensive effect were evaluated, the serum creatinine (Scr) and blood urea nitrogen (BUN) were determined. Genotyping of polymorphism of ACE2 G8790A were performed using the restriction fragment length polymorphic polymerase chain reaction (PCR-RFLP). The blood pressure and Scr and BUN levels in different genotypes were compared. RESULTS:Compared with the pre-treatment baseline blood pressure, The SBP and DBP in patients after irbesartan treatment were significantly reduced, and there was a significant statistical difference (P<0.01). At the same time, compared with the state of renal function before treatment, the Scr and BUN were both significantly reduced in patients after irbesartan treatment, and there was a statistically significant difference (P< 0.05). In terms of genotypes, ACE2 gene G8790A sites, on the other hand, there were more variations, the genotyping results show that the wild type TT 64 cases, 58 cases of TC genotype, CC genotype 13cases, the minimum allele frequency was 0.31, the genotypes distribution in accordance with hardy-weinberg equilibrium (P=0.979). Based on genotypes, the results showed that the decrease of blood pressure in patients with TT genotypes were significantly higher than that of TC and CC genotypes patients, and the results were statistically significant (P=0.018). However, there was no significant difference in the serum creatinine and blood urea nitrogen according to the three groups of genotypes. CONCLUSION: Irbesartan may help the patients with stage-2 hypertension concomitant with chronic renal failure by relieving the disease symptoms. ACE2 G8790A polymorphism may influence the antihypertensive effect of irbesartan treatment. However, there was no significant effect of the polymorphism on the clinical outcomes for irbesartan improvement in renal function.

Study of the primary target and mutation position in nemonoxacin-resistant mutants of Staphlococcus aureus

LI Zhaoxia，WANG Rui，LI Aimin，LIU Youning

2018, 23(6):  667-671.  doi:10.12092/j.issn.1009-2501.2018.06.011

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AIM: To determine the primary target of nemonoxacin and mutation position in S. aureus by genomic characterization of first and second step mutants. METHODS: Quinolone resistance-determining region(QRDR)mutations of gyrA, gyrB, and grlA of resistant mutants were identified by PCR amplification and gene sequencing. RESULTS: All the 4 first step mutants selected using nemonoxacin contained changes at codon 86, 439, and 440, but did not lead to amino acid substitution. For second-step mutants selected from first-step resistance strains, a novel second gyrA mutation (Ser-84→Leu) mutations was found. No mutations were found in grlA region of QRDR. CONCLUSION: DNA gyrase is the primary target of nemonoxacin in S. aureus. Mutation at position 84 may be the most important factor to influence the sensitivity of S. aureus.

Effects of menstrual cycle periodic Chinese medicine prescription on endometriosis infertility patients

GAO Tao, FANG Xiaohong, MA Jing, CHU Yun, CHEN Bixia

2018, 23(6):  672-677.  doi:10.12092/j.issn.1009-2501.2018.06.012

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AIM: To investigate the application of menstrual cycle periodic Chinese medicine prescription in endometriosis (EMs) infertility patients, and to provide a reference for the clinical treatment of EMs infertility patients.  METHODS: A total of 74 infertility patients with EMs from March 2014 to July 2016 who met the inclusion criteria in our hospital were selected, and were randomly divided into observation group (n=37) and control group (n=37) according to the random number table method. The control group was treated with dydrogesterone tablets, while the observation group was treated with menstrual cycle periodic Chinese medicine prescription based on the control group. The clinical effect, immune function, ovarian function and pregnancy outcome were compared between the two groups. RESULTS: The total effective rate of clinical treatment in observation group was significantly higher than that of control group (P<0.05). Before treatment, there were no significantly differences in the level of CD3⁺, CD4⁺, CD4⁺ /CD8⁺, natural kille (NK), estradiol 2 (E2) and anti-mullerian hormone (AMH) between the two groups (P>0.05). After treatment, the level of CD3⁺, CD4⁺, CD4⁺ /CD8⁺ , NK E2 and AMH were significantly better than those of before treatment (P<0.05), and the observation group was significantly better than the control group (P<0.05). Before treatment, there were no significantly differences in the level of luteinizing hormone (LH) and follicle-stimulating hormone (FSH) between the two groups (P>0.05). After treatment, the level of LH and FSH were significantly lower than those of before treatment (P<0.05), and the observation group was significantly lower than the control group (P<0.05). The success rate of pregnancy in observation group was significantly higher than that of control group (P<0.05). CONCLUSION: The menstrual cycle periodic Chinese medicine prescription can adjust the immune function, improve the ovarian function, and increase the pregnancy rate of the EMs infertile patients.

Effects of early intervention of massage combined with probiotics on neonatal jaundice of later period preterm infants

YU Jinjing, ZHANG Yanping, PAN Jiahua

2018, 23(6):  678-681.  doi:10.12092/j.issn.1009-2501.2018.06.013

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AIM: To investigate the effects of early intervention of massage combined with probiotics on neonatal jaundice of later period preterm infants. METHODS: A total of 242 later period preterm infants in neonatology department were included in this study.The infants were randomly allocated to massage group and control group. After excluding 182 cases, thirty cases in each group completed the research. Both the massage group and the control group were given bifid triple viable from first feeding time to the fourth day after birth. The massage group received massage therapy for four days from the first day postnatal while the control group received routine care. The first feeding time, the first defecation time,the daily stool frequency and the transcutaneous bilirubin level (for four days from the first day postnatal) were recorded, and the serum bilirubin level on the fourth day postnatal was also detected to investigate whether they have complications, such as ventosity, diarrhea, vomiting, infection and other complications. RESULTS: Compared with the control group, the number of defecation times of the infants in the massage group was more than that of the control group on day 2-4 after birth (P<0.05); the transcutaneous bilirubin level in the massage group was significantly lower than the control group on day 3-4 after birth (P<0.05); the serum bilirubin level of the infants in the massage group was significantly lower than that of the control group on the fourth day after birth (P<0.05). There were no complications such as ventosity, diarrhea, vomiting and infection in the two groups. CONCLUSION: Early intervention of massage combined with probiotics can significantly reduce the level of bilirubin on day 4 after birth in later period preterm infants.

Effects and safety of target controlled infusion of propofol combined with dexmetomidine in radiofrequency ablation of primary liver cancer

ZHAO Pengcheng, XU Lili, LI Yanling

2018, 23(6):  682-687.  doi:10.12092/j.issn.1009-2501.2018.06.014

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AIM: To observe the effects and safety analysis of propofol target-controlled infusion combined with dexmedetomidine in radiofrequency ablation of primary liver cancer. METHODS: Sixty-eight patients with primary liver cancer who underwent radiofrequency ablation in our hospital from February 2016 to January 2018 were randomly divided into Dex group (34 cases) and control group (34 cases). The control group was given propofol target-controlled infusion combined with remifentanil anesthesia program; on the basis of the control group, the Dex group used dexmedetomidine (first load was 0.5 μg/kg, and followed by 0.2-0.4 μg·kg^-1·h^-1 pumped). The dosage of propofol and remifentanil in two groups were compared, the intraoperative analgesia effect, anesthesia satisfaction, hemodynamics and oxygen saturation during anesthesia and intraoperative respiration adverse reactions were compared between the two groups. RESULTS: There was no significant difference of surgery time between the control group and the Dex group ( t=1.010, P=0.316), while the dosage of propofol and remifentanil as well as the waking time in the Dex group was significantly lower than those of the control group ( t=15.174, 9.703, 32.866, P=0.000, 0.000, 0.000). The BPS scores at puncture and ablation in Dex group were significantly lower than those in control group ( t=10.678, 9.451, P=0.000, 0.000), while the anesthesia satisfaction was significantly higher than that in control group ( t=6.887, P=0.000, 0.000). Compared with T0, HR, MAP, and SpO2 at T1, T2 and T3 decreased significantly in both groups ( P<0.05), and HR, MAP and SpO2 in Dex group were significantly higher than those in control group (P<0.05). Compared with T0 and T4, the HR and MAP of T2 and T3 in control group were significantly increased (P<0.05), and the HR and MAP of T2 and T3 in control group were significantly higher than that of Dex (P<0.05). There was no significant difference in HR and MAP between Dex group at T0, T2, T3 and T4 (P>0.05). The incidence of adverse respiratory and circulatory reactions in the control group was 23.53%, which was significantly higher than that in the Dex group (5.88%). The difference was statistically significant (χ²=4.221, P=0.040). CONCLUSION: Target controlled infusion of propofol combined with dexmetomidine in radiofrequency ablation of primary liver cancer can significantly reduce the dosage of propofol and remifentanil, shorten the recovery time, and have less interference with circulatory and respiratory indexes. Also, it has a lower risk of adverse reactions.

Effects of dexmedetomidine and hydroxyethyl starch combined with fentanyl on brain glucose and oxygen metabolism in elderly patients with hypertensive cerebral hemorrhage after emergency surgery

SHAO Junjin, LI Ya, LV Lei, WU Kaihua

2018, 23(6):  688-693.  doi:10.12092/j.issn.1009-2501.2018.06.015

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 AIM: To investigate the effects of dexmedetomidine, hydroxyethyl starch combined with fentanyl on brain glucose and oxygen metabolism in elderly patients with hypertensive cerebral hemorrhage after emergency surgery. METHODS: A total of 120 patients undergoing the minimally invasive aspiration of hypertensive cerebral hemorrhage with routine treatment methods in our hospital from October 2015 to May 2017 were selected, and were divided into observation group and control group according to random principle, each of 60 cases. The observation group was given dexmedetomidine, hydroxyethyl starch combined with fentanyl in anesthesia, and the control group was treated with propofol combined with fentanyl. Hemodynamics index (MAP, SBP, DBP ) were compared between the two groups; the cognitive function of the two groups was compared by MMSE (Mini-Mental state examination) before anesthesia and postoperative 1, 3 and 5days; sugar metabolism of oxygen monitoring indicators (SjvO₂, CEO₂, and A-VGlu, AVDO₂, A-VPCO₂ and A-VLac) in the two groups were monitored and compared. RESULTS: (1) During the operation and postoperative 24 hours, the heart rate, MAP, SBP and DBP of control group changed significantly (P<0.05), the observation group did not change significantly (P＞0.05), the observation group T2, T3 time heart rate, T2 time MAP, SBP and SBP were significantly better than the control group (P<0.05); (2)The MMSE score of the two groups after 1 days and 3 days, the observation group were significantly higher than those in control group (P<0.05); (3)Oxygen glucose metabolism index of the patients in the observation group (SjvO2, CEO2, A-VGlu, AVDO2, AVPCO2 and A-VLac) was significantly better than the control group (P<0.05). CONCLUSION: Anesthesia application of dexmedetomidine, hydroxyethyl starch combined with fentanyl in patients with hypertensive cerebral hemorrhage in conclusion, stable hemodynamics during operation and postoperative respiratory depression and postoperative cognitive dysfunction in low level.

Effects of Buyanghuanwu decoction on blood hypercoagulability in lung cancer patients with Qi deficiency and blood stasis

ZHENG Qingxiu，XU Xiaoxiao，LIU Gang，MA Xiaodong

2018, 23(6):  694-699.  doi:10.12092/j.issn.1009-2501.2018.06.016

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AIM: To investigate the effects of Buyanghuanwu decoction on blood hypercoagulability in lung cancer patients with Qi deficiency and blood stasis type. METHODS: Sixty cases of lung cancer patients with Qi deficiency and blood stasis were divided into observation group and control group. The control group was treated with routine nutritional support. The observation group was given Buyanghuanwu decoction on the basis of the control group. The KPS score was recorded to assess the quality of life of the patients. Symptoms of Chinese medicine were evaluated by TCM symptom score. Blood rheology indexes (whole blood viscosity, plasma viscosity, hematocrit, fibrinogen and platelet) were detected before and after treatment of the two groups. The levels of serum tPA and PAI-1 were detected before and after treatment 7, 14, 28 d in two groups. The incidence of pulmonary embolism in the two groups was recorded during the treatment.RESULTS:The curative effect of the observation group was significantly higher than that of the control group. The difference was statistically significant (P<0.05). The curative effect of TCM symptom score in the observation group was significantly higher than that in the control group. The difference was statistically significant (P<0.05). The whole blood viscosity, plasma viscosity, hematocrit, fibrinogen and platelets were significantly decreased after treatment in the observation group. And the above indexes were significantly lower than that of the control group. The difference was statistically significant (P<0.05). After 28 d of treatment, the tPA in the observation group was significantly higher than that of the control group. PAI-1 was significantly lower than that of the control group. The difference was statistically significant (P<0.05). The incidence of pulmonary embolism in the observation group was lower than that in the control group. But the difference was not statistically significant (P>0.05). CONCLUSION: Buyanghuanwu decoction can significantly improve the hypercoagulable state of patients with Qi deficiency and blood stasis type lung cancer, and improve the quality of life of patients.

Clinical efficacy of dinoprostone suppositories combined with cefuroxime in the treatment of premature rupture of membranes and effects on IL-6 and C reactive protein

HAN Yu, YANG Xinyu, CHEN Xiaolu, LU Yanmei

2018, 23(6):  700-703.  doi:10.12092/j.issn.1009-2501.2018.06.017

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 AIM: To investigate the clinical effect of dinoprostone suppositories combined with cefuroxime in the treatment of preterm premature rupture of membranes and effects on  interleukin-6 (IL-6), C reactive protein (CRP). METHODS: One hundred and four cases of premature rupture of membranes were divided into the trial group and the control group, 52 cases in each group. All patients were given intravenous infusion of cefuroxime. The control group received oxytocin to induce labor. The trial group was given dinoprostone suppositories for induction. The birth outcomes of the two groups were recorded, including vaginal delivery/cesarean section, labor time, total stage of labor, postpartum hemorrhage and intrauterine infection. Neonatal conditions were observed, including neonatal weight, Apgar score, fetal distress and neonatal asphyxia. The levels of serum CRP and IL-6 were detected in two groups at admission and after labor. RESULTS: The total effective rate of the trial group was significantly higher than that of the control group, the difference was statistically significant (P <0.05). The cesarean section, labor time, total stage of labor and intrauterine infection in the trial group were significantly lower than those in the control group, the difference was statistically significant (P<0.05). Comparison of postpartum hemorrhage volume between two groups was not statistically significant (P >0.05). Comparison of neonatal weight, Apgar score, fetal distress and neonatal asphyxia between the two groups was not statistically significant (P >0.05). CRP in the two groups increased significantly after parturition, and IL-6 decreased significantly (P <0.05). The levels of CRP and IL-6 in the study group were significantly lower than those in the control group, the difference was statistically significant (P <0.05). CONCLUSION: Dinoprostone suppositories combined with cefuroxime has definite effect on the treatment of preterm premature rupture of membranes, and can increase the rate of vaginal delivery, reduce the rate of intrauterine infection.

Pharmaceutical service in statinsinduced Rhabdomyolysis by clinical pharmacists

YUAN Yumei, WANG Ronghua, YANG Shuixin

2018, 23(6):  704-708.  doi:10.12092/j.issn.1009-2501.2018.06.018

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AIM: To explore the pharmaceutical services of clinical pharmacists in rhabdomyolysis caused by statins. METHODS: Clinical pharmacist participated in the assessment and treatment of three patients of rhabdomyolysis caused by statins, analyzed and summarized the pharmaceutical services of clinical pharmacists in it. RESULTS: Clinical pharmacists provided Pharmaceutical Care of rhabdomyolysis caused by statins, conducted the relevance evaluation of Adverse Drug Reactions, analyzed the risk factors of rhabdomyolysis caused by statins, and offered corresponding pharmaceutical services. CONCLUSION: Clinical pharmacists can provide pharmaceutical service on entry point of relevance evaluation of Adverse Drug Reactions, focusing on risk factors, giving Medical education in rhabdomyolysis patients induced by statins.

Progress in neurosteroids related to the pathogenesis and treatment of schizophrenia

CAO Ting, LI Nana, CAI Hualin

2018, 23(6):  709-714.  doi:10.12092/j.issn.1009-2501.2018.06.019

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The latest researches have shown that the metabolism of neurosteroids is probably involved in the pathogenesis of schizophrenia and associated with relevant treatment. This review will focus on the relationship between neurosteroids and schizophrenia, mainly from these perspectives: biosynthesis of neurosteroids, alternation of neurosteroids, mechanism of action and its auxiliary role in schizophrenia, gender differences etc. so as to provide theoretical references for applying neurosteroids in adjuvant treatment of schizophrenia.

Advances in pathogenesis of nonalcoholic fatty liver disease caused by tamoxifen

CHEN Xi, LING Jiawei, DING Jiaxin, JIANG Zhenzhou, ZHANG Luyong

2018, 23(6):  715-720.  doi:10.12092/j.issn.1009-2501.2018.06.020

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Tamoxifen, a selective estrogen receptor antagonist, is most widely used for endocrine therapy for breast cancer. However, mounting evidence suggests that tamoxifen treatment can significantly improve the risk of nonalcoholic fatty liver disease (NAFLD) in breast cancer patients. At present, there is no enough research on hepatic lipotoxicity caused by tamoxifen, the exact mechanism of it is still unknown. In this paper, we reviewed the phenomenon and mechanism of tamoxifen induced nonalcoholic fatty liver disease, providing references for further mechanism research and therapeutic drugs development.