Long non-coding RNA TSIX regulates the proliferation of human pancreatic cancer cells by targeting miR-384

doi:10.12092/j.issn.1009-2501.2018.06.004

Abstract

Abstract:

AIM: To observe the expression levels of long non-coding RNA (LncRNA-TSIX) in the pancreatic cancer tissues and cell lines, and to explore its effect on cell proliferation and its possible mechanism. METHODS: qPCR was used to detect the expression of TSIX in 5 pancreatic cancer cell lines (BxPC-3, CAPAN-1, AsPC-1, CFPAC-1 and SW1990) and human normal pancreatic ductal epithelial cells HPDE6-C7, and 7 pancreatic cancer tissues with para-cancer tissues were also collected. Bioinformatics prediction and analysis of miRNAs and downstream genes paired with TSIX were performed. SW1990 cells were infected with recombinant lentiviral vector carrying TSIX siRNA gene (LV-siRNA) as experimental group and negative control lentivirus particles (LV-NC) as control group. The effect of TSIX low-expression on miR-384 and Gab2 mRNA expression was detected by qPCR, and the expression of Gab2 and PI3K/AKT pathway related protein was determined by Western blot. The effects of TSIX low-expression on the cell cycle, viability and colony formation ability of SW1990 cells were detected by flow cytometry, MTT assay and colony formation assay. RESULTS:Compared with HPDE6-C7 cells, TSIX was highly expressed in the pancreatic cancer cell lines (P<0.01). The expression of TSIX was the highest in the SW1990 cells (P<0.01). The expression level of TSIX in pancreatic cancer tissues was higher than that in its adjacent tissues (P<0.05). The expression of TSIX in the SW1990 cells transfected with TSIX siRNA was significantly decreased (P<0.01), the expression of miR384 was increased (P<0.01), and the mRNA expression of Gab2 was decreased (P<0.01). Low expression of TSIX significantly inhibited the cell cycle, and inhibited the cell viability and colony formation ability (P<0.01). CONCLUSION: LncRNA TSIX is overexpressed in the pancreatic cancer tissues and cell lines. Knockdown of TSIX expression inhibits the proliferation of SW1990 cells. The possible mechanism is that TSIX regulates the expression of Gab2 gene by combining with miR-384.

Key words: long non-coding RNA, microRNA, pancreatic tumor, cell proliferation

CLC Number:

R965.2

SHEN Binbin, ZHOU Jun, LI Liang, LU Ning, YAO Ming. Long non-coding RNA TSIX regulates the proliferation of human pancreatic cancer cells by targeting miR-384[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(6): 621-626.

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