Study of the primary target and mutation position in nemonoxacin-resistant mutants of Staphlococcus aureus

doi:10.12092/j.issn.1009-2501.2018.06.011

Abstract

Abstract:

AIM: To determine the primary target of nemonoxacin and mutation position in S. aureus by genomic characterization of first and second step mutants. METHODS: Quinolone resistance-determining region(QRDR)mutations of gyrA, gyrB, and grlA of resistant mutants were identified by PCR amplification and gene sequencing. RESULTS: All the 4 first step mutants selected using nemonoxacin contained changes at codon 86, 439, and 440, but did not lead to amino acid substitution. For second-step mutants selected from first-step resistance strains, a novel second gyrA mutation (Ser-84→Leu) mutations was found. No mutations were found in grlA region of QRDR. CONCLUSION: DNA gyrase is the primary target of nemonoxacin in S. aureus. Mutation at position 84 may be the most important factor to influence the sensitivity of S. aureus.

Key words: nemonoxacin, staphlococcus aureus, drug resistance, mutation position

CLC Number:

R968

LI Zhaoxia，WANG Rui，LI Aimin，LIU Youning. Study of the primary target and mutation position in nemonoxacin-resistant mutants of Staphlococcus aureus[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(6): 667-671.

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Study of the primary target and mutation position in nemonoxacin-resistant mutants of Staphlococcus aureus

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