NBP inhibits ox-LDL induced apoptosis of endothelial cells through endoplasmic reticulum stress pathway

doi:10.12092/j.issn.1009-2501.2019.04.006

Abstract

Abstract:

AIM: To investigate the effect of N-butylphthalide (NBP) on endothelial cell apoptosis induced by low density lipoprotein (ox-LDL) through endoplasmic reticulum stress pathway. METHODS: Human umbilical vein endothelial cells (HUVECs) were cultured in vitro, pretreated with NBP (5, 10, 20 mg/L) and 4-phenylbutyric acid (4-PBA, 4 mmol/L) for 1 hour, and then cultured with ox-LDL (100 mg/L). CCK-8 assay was used to detect cell viability, flow cytometry to detect apoptosis, LDH kit to detect the level of lactate dehydrogenase (LDH) in culture medium, Hoechst 33342 staining to observe the level of apoptosis. Western blot and RT-QPCR were used to detect the expression levels of CHOP and GRP78 in the endoplasmic reticulum stress signal. RESULTS:ox-LDL could induce the activation of ER stress pathway in HUVECs and promote the apoptosis of HUVECs. NBP inhibited the injury of HUVECs induced by ox-LDL in a dose-dependent manner, increased the cell viability, decreased the apoptosis rate and decreased the level of LDH. NBP, similar to PBA, could inhibit the expression of CHOP and GRP78 and the activation of ER stress.CONCLUSION: NBP can inhibit the apoptosis of HUVECs induced by ox-LDL, and the mechanism is related to the inhibition of ER stress pathway.

Key words: N-butylphthalide, low density lipoprotein, endoplasmic reticulum stress, human umbilical vein endothelial cells

CLC Number:

R965.2

GUAN Qiaobing，YANG Yi，GUO Li，HAN Chenyang. NBP inhibits ox-LDL induced apoptosis of endothelial cells through endoplasmic reticulum stress pathway[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 397-402.

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