Resveratrol protects human retinal pigment epithelial cells from toxicity of high glucose by up-regulation of miR-26a

doi:10.12092/j.issn.1009-2501.2020.03.007

Abstract

Abstract: AIM: To study the protective effects and mechanism of resveratrol (RES) on the injury of human retinal pigment epithelial cells (ARPE-19) induced by high glucose (HG). METHODS: ARPE-19 cells were cultured in HG to simulate injury. Cell viability, apoptosis, ROS generation and miR-26a level were examined by CCK-8 assay, flow cytometry assay, DCFH-DA staining and RT-qPCR, respectively. Expression of proteins associated with viability, apoptosis and oxidative stress was measured by Western blot analysis. In addition, the involvements of the ERK and Wnt/β-catenin pathways were analyzed by Western blot analysis.RESULTS:HG reduced cell viability while promoted apoptosis and oxidative stress in ARPE-19 cells. RES ameliorated HG-induced cell injury. The expression of miR-26a was up-regulated by RES in HG-treated cells, and miR-26a inhibition obviously reversed the effects of RES on HG-treated cells. Finally, we found the ERK and Wnt/β-catenin pathways were inhibited by RES through up-regulation of miR-26a. CONCLUSION: RES protected ARPE-19 cells against HG-induced injury through up-regulating miR-26a, along with inhibition of the ERK and Wnt/β-catenin pathways. RES might be a potential therapeutic drug for diabetic retinopathy.

Key words: resveratrol, high glucose, human retinal pigment epithelial cells, miR-26a

CLC Number:

ZHOU Haiyan, WANG Kailing . Resveratrol protects human retinal pigment epithelial cells from toxicity of high glucose by up-regulation of miR-26a[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(3): 285-292.

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