Abstract: Aim In order to investigate the protective effects of Silybin and Polyporus Umbellalus Polysaccharides (PSP) on the ultrostructure of human hepatocytes a new experimental model system was founded by human fetal hepatocytes injued by CCL. Methods The hepatocytes the liver of human fetus aged. between 5 and 7 monthes were pretreated by collegenase digestion, treated with Silybin and PSP for different times and then exposed to CCL for 4 hours. Under scanning electron microscope (SEM) the hepatocytes were observed and the transaminase. and SOD in the culture medium were also assayed at the end of culture. Results The levels of transaminaese ALT and AST were largely decresed and the SOD level was elevated in culture medium in two medicines groups when compared with that in control group. And also the cellular integrity and membrane ultrostructure of hepatocytes were well preserved in two drugs groups. Conclusion Polyporus Um bellalus Ploysaccharides (PSP) and Silybin are good drugs for treatment of the liver damage. This model system founded by human fetal hepato-cytes injured by CCL could acl as one of useful methods for observing the effects of drugs on human hepatocytes.

Key words: Polyporus umbellalus polysaccharides(PSP), Silybin, Human fetal hepatocytes