Abstract: Aim To observe the effect of recombinant human interferon and verazole used alone or in combination in resisting respiratory synthesis virus (RSV) in vitro. Methods RSV strains were proliferated with Hela cells in Eagles solution on a 96-hole plate. The recombinant human interferon and virazole were diluted to different concentrations and were separately added in the dose of 100 μl to each hole of the plate. After 48 hours cultured, the concentrations of the drugs for inhibiting cytopathogenic effect (CPE) of RSV were determined. Results When the concentration of interferon was ≥5 U·ml^-1 and virazlole ≥ 24 μg·ml^-1, respectively, the effect of the two drugs on inhibiting the CPE of RSV was remarkable and was improved with their concentration increasing. When the concentrations of the two drugs were lower than that of their effect respectively, their united use also had obvious effect in resisting the virus. In addition, the different using methods of interferon have also different results. Conclusion Both recombinant human interferon and virazole are effective in inhibiting RSV in vitro and will bring about better effect when used in combination.

Key words: resisting virus in vitro, interferon, virazole, respiratory synthesis virus