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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2002, Vol. 7 ›› Issue (5): 385-388.

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Relationship between the protective effect of sodium oxybate on neuronal damage induced by hypoxia-reoxygenation and GABAA receptor in primary cultured rat cortical neurons1

GU Shu-Ling, LIMei, CUI Jia-Yong, YAO Bin, DAI Ti-Jun2   

  1. Department of Pharmacology, Xuzhou Medical College, Xuzhou 221002;
    2Jiangsu Provincial Key Laboratory of Anesthesiology, Xuzhou 221002
  • Received:2002-07-15 Revised:2002-07-26 Published:2020-11-26

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Abstract: AIM: To investigate the relationship between the protective effect of sodium oxybate on neuronal damage induced by hypoxia-reoxygenation and GABAA receptor in primary cultured rat cortical neurons. METHODS: The primary cultured rat cortical neurons were used to make the hypoxia-reoxygenation damage model.The morphology of cell was observed.The lactate dehydrogenase (LDH) effluxed into the media as an indicator of neuronal injury was detected after 6 h of the reoxygenation injuries.The malonyldialdehyde (MDA) contents, superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities were determined at the same time. RESULTS: The hypoxia-reoxygenation caused neuronal swelling and widespread neuronal degeneration, increased LDH efflux and MDA contents, and decreased SOD and GPX activities.Sodium oxybate assuaged neuron damage, decreased LDH efflux and MDA contents (P <0.01), and increased SOD and GPx activities (P <0.01).The effect could be abated by seurinine (P <0.01). CONCLUSION: The protective effect of sodium oxybate on neuronal damage induced by hypoxia-reoxygenation is related to excited GABAA receptor.

Key words: sodium oxybate, seurinine, GABAA receptor, hypoxia-reoxygenation injury

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