Effects of 1-methy-4-phenylpyridinium and 6-hydroxydopamine on the glutamate uptake into cultured C6 glioma cells1

Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2002, Vol. 7 ›› Issue (6): 481-484.

Effects of 1-methy-4-phenylpyridinium and 6-hydroxydopamine on the glutamate uptake into cultured C6 glioma cells¹

YAO Hong-Hong, ZHANG Yun², HE Hai-Rong, DING Jian-Hua, LI Hao, HU Gang

Department of Pharmacology &Neurobiology,Nanjing Medical University,Nanjing 210029;
²Department of Pharmacology,Zhenjiang University,Zhenjiang 212001

Received:2002-09-27 Revised:2002-10-02 Online:2002-12-26 Published:2020-11-20

Abstract

Abstract: AIM: To study the effects of 6-OHDA and MPP⁺on the glutamate uptake into cultured C6 glioma cells.METHODS: The glutamate uptake into C6 glioma cells was measured by using radioimmunoassay method;and the viability of C6 glioma cells was investigated by MTT method.RESULTS: Both 6-OHDA and MPP⁺inhibited glutamate uptake into C6 glioma cells in a concentration-dependent manner.Treatment with 6-OHDA produced inhibitory effect on the viability of C6 glioma cells.However,exposure to MPP⁺showed no such effect.Incubation with TPA,a PKC activator,caused increase in glutamate uptake and completely reversed MPP⁺-induced uptake inhibition.CONCLUSION: Inhibition of glutamate uptake by MPP⁺was completely reversed by TPA,indicating that the effect of MPP⁺was mediated by PKC cell signal transduction,while the inhibitory effect of 6-OHDA was due to the impairment viability of C6 glioma cells.

Key words: 6-OHDA, MPP⁺, C6 glioma cells, glutamate uptake

CLC Number:

R971

YAO Hong-Hong, ZHANG Yun, HE Hai-Rong, DING Jian-Hua, LI Hao, HU Gang. Effects of 1-methy-4-phenylpyridinium and 6-hydroxydopamine on the glutamate uptake into cultured C6 glioma cells¹[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2002, 7(6): 481-484.

References

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Effects of 1-methy-4-phenylpyridinium and 6-hydroxydopamine on the glutamate uptake into cultured C6 glioma cells¹

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