Effect of hypertonic-hyperoncotic solution on cell volume and survival rate in human umbilical vein endothelial cells suffering from hypoxia/reperfusion

Abstract

Abstract: AIM: To investigate the effect of hypertonic-hyperoncotic solution(HHS)on cell volume and survival rate in human umbilical vein endothelial cells(HUVEC)suffering from hypoxia/reperfusion.METHODS: HUVEC of ECV-304 were selected and randomly divided into control grouPand experimental group.Cells in two groups were cultured in hypoxia environment(95 %nitrogen,and 5 % carbon dioxide)for 8 h,then in normal environment(95 % air,and 5 % carbon dioxide)for 16 h in control group,and in HHS for 15 min in experimental group.The cell volume and the survival rate were detected before experiment,15 min,60 min,8,8.25,16,and 24 h after experiment in two groups.RESULTS: In control group,cells became significantly inflated and the survival rate decreased in hypoxiaPeriod afterlasting 60 min and hypoxia/reperfusionPeriod(P<0.05 orP<0.01).In experimental group,cells became deflated significantly compared with control grouPat the samePeriod(P<0.01).The survival rate decreased,but significantly higher than that in control grouPat the samePeriod(P<0.05).CONCLUSION: HHS can delay RVD,keePcell volume steady,and decrease cell mortality suffering from hypoxia/reperfusion.

Key words: pharmacodynamics, hypertonic-hyperoncotic solution, human umbilical vein endothelial cells, hypoxia/reperfusion, cell volume, survival rate

CLC Number:

HUANG Shao-Nong, CHEN Jian-Yan, YUAN Shi-Ying. Effect of hypertonic-hyperoncotic solution on cell volume and survival rate in human umbilical vein endothelial cells suffering from hypoxia/reperfusion[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(4): 447-450.

References

1 Shatos MA,Doherty JA,StumPDC,et al.Oxygen radicals generated during anoxia followed by reoxygenation reduce the synthesis of tissue-typePlasminogen activator andPlasminogen activator inhibitor-1 in human endothelial cell culture[J].J Biol Chem,1990;265:20443-8
2 Kröll W,Pölz W,Colombo T,et al.“Small volume resuscitation”-Erö ffnen sich damit neue M? glichkeiten in der Behandlung des hypovolömischen Schocks[J] ?Wien KlinWochenschr,1994;106:8-12
3 Kletzien RF,Pariza MW,Becker JE,et al.A method using 3-O-Methyl-D-Glucose andPhloretin for the determination of intracellular water space od cells in monolayer culture[J].Anal Biochem,1975;68:537-44
4 Fuijita H,Kuroda Y,Saitch Y.The mechanism of action of the two-layer cold storge method in caninePancresaPreservationprotection ofPancreatic microvascular [J].Kobe J Med Sci,1995;41:47-61
5 WatkinsMT,Haudenschild CC,Al-Badawi H,et al.Immediate responses of endothelial cells to hypoxia and reoxygenation:an in vitro model of cellular dysfunction[J].Am JPhysio,1995;268:749-58
6 Kempski O,Behmanesh S.Endothelial cell swelling and brainPerfusion[J].J Trauma,1997;42:38-40
7 Mazzoni MC,BorgstromP,Warnke KC,et al.Mechanisms and implications of capillary endothelial swelling andluminal narrowing inlow-flow ischemias[J].Int J Microcirc Clin Exp,1995;15:265-70
8 袁世荧,张雪平,曾邦雄.高晶体-高胶体渗透压混合液对脂多糖刺激的人血管内皮细胞ICAM-1mRNA 表达的影响[J].中华麻醉学杂志,2002;22:283-6
9 Corso CO,Okamoto S,Leiderer R,et al.Resuscitation with hypertonic saline dextran reduces endothelial cell swelling and improves hepatic microvascularPerfusion and function after hemorrhagic shock[J].J Surg Res,1998;80:210-20
10 Vollmar MD,Preissler G,Menger MD.Small-volume resuscitation restores hemorrhage-induced microcirculatory disorders in ratPancreas[J].Crit Care Med,1996;24:445-50
11 lang F,Vlkl H,Hussinger D,et al.GeneralPrinciples in cell volume regulation[A].In:Bey enbach K Weds,ed.Cell volume regulation[M].1th ed.Basel:Karger,1990:1-16
12 Kimel berg HK,Goderie S,Khigman S,et al.Swelling-induced release of glutamate,aspartate,and taurinefrom as trocytecultures[J].J Neurosci,1990;10:1583
13 Mc Manus ML,Srange K.Acute volume regulation of brain cells in response to hypertonic challenge[J].Anesthesiology,1993;78:1132
14 lippmann BJ,Yang R,Barnett DW,et al.Pharmacology of volume regulation following hypotonicity-induced cell swelling in clonal N1E115 neuroblas to macells[J].Brain Res,1995;686:29

[1]	WU Yujie, ZHAO Chengcheng, XI Qing. Evaluation of tegacycline regimens in treatment of gram-negative bacterial infections with Monte Carlo simulation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(9): 1027-1033.
[2]	GU Yifei, CHU Nannan, HUANG Kai, QUE Linling, ZHANG Jisheng, XIANG Xuemei, HE Qing. Application and progress of pharmacodynamics study in bioequivalence evaluation of orally inhaled drug products [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(7): 822-833.
[3]	ZHANG Jinhua, LIU Maobai, CAI Mingzhi, ZHENG Yingli, LAO Haiyan, XIANG Qian, DU Liping, ZHU Zhu, DONG Jing, ZUO Xiaocong, LI Xingang, SHANG Dewei, CHEN Bing, YE Yanrong, WANG Yuzhu, GAO Jianjun, ZHANG Jian, CHEN Wansheng, XIE Haitang, JIAO Zheng. Model informed precision dosing of warfarin: China expert consensus report (2022 version) [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(11): 1201-1212.
[4]	XU Liying, ZHANG Gaosong, HUANG Xiaomin, HUANG Jihan, WANG Shuhua. Clinical efficacy and modeling evaluation of He-wei-zhi-xie capsules in treating diarrhea patients [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(1): 47-55.
[5]	JIAO Zheng, LI Xingang, SHANG Dewei, DONG Jing, ZUO Xiaocong, CHEN Bing, LIU Jianmin, PAN Yan, ZHOU Tianyan, ZHANG Jing, LIU Dongyang, LI Lujin, FANG Yi, MA Guangli, DING Junjie, ZHAO Wei, CHEN Rui, XIANG Xiaoqiang, WANG Yuzhu, GAO Jianjun, XIE Haitang, HU Pei, ZHENG Qingshan. Model informed precision dosing: China expert consensus report [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(11): 1215-1228.
[6]	LI Wenchao, BAI Xiangrong, LI Xiaoling, JIANG Dechun. Population pharmacokinetics/pharmacodynamics of tigecycline in the treatment of different infectious diseases [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(11): 1265-1272.
[7]	XIANG Rongfeng, SUN Fengjun, XIONG Lirong, YU Mingjie, DAI Qing, CHEN Yongchuan. Pharmacokinetics and pharmacodynamics of (S)-pantoprazole sodium enteric-coated tablets in healthy subjects [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(8): 903-909.
[8]	LI Jian, YANG Jinbo, WANG Yuzhu. Applications of model-informed drug development (MIDD) on new drug research and development [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(1): 1-8.
[9]	QUAN Haiyan, LIU Yuhuan, YANG Li, YANG Fang, QIN Xuping. Improvement of CGRP on vascular endothelial cells insulin resistance via activation of cAMP/PPARγ/eNOS pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 615-622.
[10]	GUAN Qiaobing，YANG Yi，GUO Li，HAN Chenyang. NBP inhibits ox-LDL induced apoptosis of endothelial cells through endoplasmic reticulum stress pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(4): 397-402.
[11]	MA Guangli, XU Ling, CHEN Rui, CHEN Yuancheng, ZHAO Wei, LIU Dongyang, JIAO Zheng, LI Jian, JI Shuangmin, LI Li, LI Liang, WANG Yuzhu, YANG Jinbo, WANG Yaning, SUN He, HU Pei, ZHENG Qingshan, LU Wei. General considerations of population pharmacokinetic/pharmacodynamic study in new drug development [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(11): 1201-1220.
[12]	LIAN Yanhong, JIANG Huifang, YUAN Xiaohong, ZHOU Huidan, XIE Kangjie, FANG Jun, JIN Xiaoju. Effects of neoadjuvant chemotherapy on the pharmacodynamics of rocuronium bromide in patients undergoing gastric cancer surgery [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(10): 1161-1164.
[13]	. Effects and mechanisms of nicotinic acid curcumin on apoptosis induced by low shear stress in human umbilical vein endothelial cells [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(8): 855-861.
[14]	ZHANG Chao . Pharmacodynamic studies evaluation of voriconazole [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(1): 116-120.
[15]	WU Yonglei, WANG Dan, ZHANG Suiyang, WANG Ying, SUN Qin. Pharmacokinetics and pharmacodynamics of meropene administered by traditional prolonged infusion or optimized prolonged infusion in different tissues of septic rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2017, 22(7): 767-774.