Inhibition of dexamethasone on degranulation of mast cells and its mechanism

Abstract

Abstract: AIM: To study the direct effect of dexamethasone on the degranulation of mast cells.METHODES:The flow cytometry was used to observe the effect of dexamethasone on the degranulation of RBL-2H3 cellline(rat basophileleukemia cellline),and the changes of ERK1/ERK2 signal transduction characteristics was analyzed by western blot.RESULTS:Pre-treating RBL-2H3 cellline with dexamethasone resulted in the decrease of Annexin VPositive staining of cells and the activity of MAPK compared that of the activated group.CONCLUSION: The inhibition of dexamethasone on mast cells is associated with the activity of MAPK.

Key words: dexamethasone, mast cells, RBL-2H3, degranulation, Annexin V, MAPK

CLC Number:

R977.1
R965

YUANLI, GUO Wei, LIU Shui-Ping. Inhibition of dexamethasone on degranulation of mast cells and its mechanism[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2003, 8(6): 642-644.

[1]	ZHU Ruifang, GUO Dongkai, ZHI Hui, JIANG Yiguo, ZHANG Yueling, QIAN Xiaoping, JI Shiliang. Exploring the mechanism of elemene synergistic bortezomib against multiple myeloma by ROS-NF-κB-p38MAPK pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2023, 28(11): 1219-1226.
[2]	JIN Yiyi, ZHOU Keting, YANG Chengcheng, XU Ping, ZHU Suyan. Osthole attenuates diabetes-induced renal injury by regulating NF-κB and p38/MAPK pathway mediated inflammatory responses [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2022, 27(6): 622-631.
[3]	ZHAN Zhuqin, BAI Haitao. Effects of different doses of dexamethasone on angiotensin II, its receptors and NO levels in septic kidney injury rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(9): 995-1004.
[4]	WU Wei, LI Guangpeng, ZHANG Jiangbo, KONG Lingyu, CAI Junyan, YANG Feiyun. Gastrodin combined with dexamethasone protects H9C2 cell from injury induced by oxygen-glucose deprivation [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2021, 26(11): 1244-1249.
[5]	ZHANG Cheng, FAN Manli, WANG Kun, QIN Zhiqiang, ZHOU Lanlan. Protective effect of dexamethasone implant on nephrotoxicity of adriamycin in rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(6): 610-617.
[6]	CAI Jiangyi, LIU Yao, LI Jiacheng. Temperature selection of intravesical sodium hyaluronate in the treatment of interstitial cystitis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2020, 25(4): 447-454.
[7]	YU Jiaojiao, YANG Yang, LIU Ying, GUO Xiaoxi, XU Tianrui, AN Shu. Paeoniflorin inhibits mast cell activation and degranulation through PI3K/AKT/m-TOR signaling pathway [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(9): 961-968.
[8]	LI Min, WANG Shanshan, LIU Zhonghe, JIANG Deguo. Expression and mechanism of p38MAPK signaling pathway in chronic pulpitis rats with pain [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(6): 644-649.
[9]	DU Tianping, MENG Zudong. Mechanism of 17β-estradiol on p38MAPK signal transduction pathway in propofol-induced hippocampal neuronal apoptosis in developing rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(2): 128-133.
[10]	KONG Lingyu, XI Zan, YANG Yaqin, MA Wenting, WU Wei, HAO Tongqin. Effects of dexamethasone on the Notch signaling pathway and apoptosis-associated genes in acute pancreatitis [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(2): 134-139.
[11]	BAI Xuemin, ZOU Likao, JIN Zhenmu, JIANG Lijia. Effects of N-acetylcysteine on regulation of EGFR/MAPK signaling pathway in airway mucus hypersecretion in chronic obstructive pulmonary disease [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(10): 1120-1127.
[12]	WANG Jing, ZHOU Haibin, GU Weigang, WANG Xia, YANG Jianfeng. Effect of ketotifen on gastrointestinal symptoms, visceral sensitivity and intestinal mast cells in patients with irritable bowel syndrome with diarrheal [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2019, 24(1): 63-70.
[13]	YAO Xinming, KONG Xiang, ZHAO Yongli, HUA Qiang, HE Chunling, YU Dan, LI Yeqiong, DAI Xiaokang, YE Shandong. Metformin inhibits p38MAPK signaling pathway and oxidativestress in rat glomerular mesangial cells cultured with high glucose [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(6): 614-620.
[14]	CHENG Xiaolong, HU Kunmei, HU Haoran，XU Hanghang, YANG Jieren, HAN Jun. Effects of Chinese medicine Yuxia capsule on renal fibrosis in spontaneously hypertensive rats [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(6): 632-639.
[15]	LOU Chaosheng, WU Xian, WANG Haibo, ZHANG Yin, YE Lechi. Therapeutic effect and mechanism of Yipi Yangwei decoction in treating ulcerative colitis-related cancerous mice [J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(10): 1088-1096.

Inhibition of dexamethasone on degranulation of mast cells and its mechanism

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments