Effects of iptakalim hydrochloride on apoptosis of primary cultured cortical neurons induced by hypoxia

Abstract

Abstract: AIM: To investigate the effects of iptakalim hydrochloride (Ipt) on apoptosis of primary cultured cortical neurons induced by hypoxia.METHODS: Apoptosis was measured using electron microscope and flow cytometric analysis technique.The expression of Bcl-2 and Bax was revealed by immunocytochemistry.RESULTS: Ipt 10 μmol·L^-1 was found to improve ultrastructural features of apoptosis of primary cultured cortical neurons induced by hypoxia;up-regulate Bcl-2 expression with no influence on Bax expression.Ipt (0.1-10 μmol·L^-1)was shown to decrease the percent of apoptosis dose-dependently.These effects were antagonized by KATP blocker glibenclamide (Gli).CONCLUSION: Ipt showed inhibitory effects on apoptosis of primary cultured cortical neurons induced by hypoxia and its mechanisms may be related with KATP and Bcl-2 expression.

Key words: apoptosis, iptakalim hydrochloride, ATP-sensitive potassium channel, glibenclamide, hypoxia

CLC Number:

R972.4
R962

ZHANG Ying-Li, FENG Hua-Song, WANG Hai. Effects of iptakalim hydrochloride on apoptosis of primary cultured cortical neurons induced by hypoxia[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(1): 20-24.

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