Abstract: AIM: To investigate the antiaging effects of ginsenoside Rg1 and the possible mechanism of cell cycle in senescence model induced by tert-butylhydroperoxide (t-BHP).METHODS: WI-38 cells were divided into 4 groups and randomly added with different concentrations of Rg1.From 30 population doubling (PD), WI-38 cells were exposed to t-BHP for 1 h at every two PDs. Four stresses were induced to reach premature senescence.The changes of cells of were observed by microscope and flow cytometric assay, and the SA-β-galactosidase staining was used to investigate the antiaging effect of ginsenoside Rg1.The expression of CDK4, cyclin D1 and p16 was detected by Western blot.RESULTS: Cells of Rg1 groups were smaller and less flat than those in the group only treated with t-BHP. In addition, the number of secondary lysosome, cells in G₁ phase, and activity of β-galactosidase decreased.Futhermore it showed higher expression of CDK4 and lower expression of cyclin D1 and p16 in comparison with cells only treated with t-BHP.CONCLSION: The possible mechanism of antiaging effect of Rg1 is to regulate the expression of p16, cyclin D1 and CDK4 In senescence model induced by t-BHP.

Key words: ginsenoside Rg1, p16, cyclin D, CDK4, senescence, tert-butylhydroperoxide