Abstract: AIM: To study the timed number of nitric oxide synthase (NOS)-positive cells and the effects of sodium magnesium fructose diphophate (FDPM).METHODS: Rat model of intracerebral hemorrhage (ICH) was induced by collagenase, the timed number of NOS-positive cells and the effects of FDPM on it was determined by NADPH histochemical staining.RESULTS: After the onset of ICH, the number of NOS-positive cells increased obviously at 2 hours, decreased lightly at 6 hours, increased again at 12 hours, peaked during from 24 hours to 3 days, and decreased again at 7 days, but was till higher than that of normal condition.Application of FDPM decreased significantly the number of NOS-positive cells after ICH, and the effect of FDPM was superior to that of fructose-1, 6-diphophate or magnesium sulfate.CONCLUSION: The change of NOS activity shows two peaks after ICH, and inhibition of NOS activity may be one of the important protective mechanisms of FDPM from ICH.

Key words: intracerebral hemorrhage, nitric oxide synthase, sodium magnesium fructose diphophate, fructose-1, 6-diphophate, magnesium sulfate