Effects of iptakalim hydrochloride on glutamate receptors and spontaneous synaptic activities in rat hippocampal neurons

Abstract

Abstract: AIM: To investigate the effects of iptakalim hydrochloride (Ipt) on glutamate receptors and spontaneous synaptic activities in rat hippocampal neurons.METHODS: The cultured hippocampal neurons from neonatal rats were served as preparation.The effects of Ipt on glutamate receptors and spontaneous synaptic activities were observed by using whole-cell recording technique .RESULTS: Different concentrations of Ipt (1-100 μmol·L^-1) suppressed the glutamate and NMDA induced currents of rat hippocampal neurons in a dose-dependent manner, and these inhibitory effects were antagonized by K_ATP blocker glibenclamide (30 μmol·L^-1).Ipt inhibited the sEPSCs in cultured rat hippocampus neurons, and decreased both the frequency and amplitude, with no effect on mEPSCs.CONCLUSION: Ipt can block the function of glutamate receptor and attenuate the glutamatergic transmission in cerebral neurons, and these effects may be related with K_ATP.

Key words: iptakalim, hippocampus, neuron, glutamate, NMDA, patch clamp

CLC Number:

R965
R971

ZHANG Ying-Li, WANG Hai. Effects of iptakalim hydrochloride on glutamate receptors and spontaneous synaptic activities in rat hippocampal neurons[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(2): 130-135.

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