Relationship between protective effect and antioxidative action of rutosid on experimental acute pancreatitis in rats

Abstract

Abstract: AIM: To study the protective effect and the antioxidative action of rutoside (Ru) on experimental acute pancreatitis (AP) in rats.METHODS: AP model was induced by retrograde injection of sodium deoxycholate into bilipancreatic duct in rats.The amylase (AMS) activity and the nitric oxide (NO) level in serum were measured at 6 and 12 h after the induction of AP. The superoxide dismutase (SOD) activity and the malondialdehyde (MDA) content in pancreas tissue were measured at 24 h after the induction.Meanwhile, the pathologic change of pancrease at 24 h after the induction was observed.RESULTS: Ru (30, 60, and 120 mg·kg^-1, sc) could decrease the serum levels of AMS and NO significantly, attenuate the pathologic leision of pancrease, enhance the SOD activity and reduce the MDA production in the pancreas tissue.CONCLUSION: Ru has certain protective effect on AP in rats by administrating subcutaneously.The mechanisms may be due to strengthening endogenous antioxidative system and the ability of scavenging oxygen-derived free radicals (OFR), improving the histophysiology of AP and attenuating the injury of pancreas.

Key words: rutoside, acute pancreatitis, serum amylase, nitric oxide, superoxide dismutase, malondialdehyde

CLC Number:

R965.1

TIAN Jie, SONG Shao-Gang, CHEN Zhi-Wu, ZHAO Wei-Zhong. Relationship between protective effect and antioxidative action of rutosid on experimental acute pancreatitis in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(4): 455-458.

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