MiR-145 regulating SOD activity mediated brain protection of ginsenosides Rb1 in rats with cerebral ischemia-reperfusion injury

doi:10.12092/j.issn.1009-2501.2018.11.003

Abstract

Abstract:

AIM: To explore whether ginsenosides Rb1 can increase the activity of SOD in the brain tissue of rats with cerebral ischemia reperfusion injury by reducing the miR-145 content, and play the role of brain protection. METHODS: Sixty SD rats were randomly divided into five groups: model group, normal saline control group, ginsenosides Rb1 group, ginsenosides Rb1+miRNA-145 mimics group and ginsenosides Rb1+miRNA-145 mimics NC group, 12 rats in each group. The rats' cerebral ischemia reperfusion injury models were established by thread embolism method to form middle cerebral artery occlusion (MCAO). After modeling, the rats in ginsenosides Rb1 and saline control group were given ginsenosides Rb1 and normal saline respectively. Two days before modeling, the rats in ginsenosides Rb1+miRNA-145mimics and ginsenosides Rb1+miRNA-145 mimics NC group received miRNA-145 mimics and miRNA-145 mimics NC through lateral ventricle, and other operations were same to ginsenosides Rb1 group. Twenty-four hours after cerebral ischemia reperfusion injury, the cerebral tissues were taken and the volume of cerebral infarction was measured in 6 rats in each group. The cerebral cortex was taken from another 6 rats in each group after execution to detect the contents of miR-145 and superoxide dismutase (SOD). RESULTS: Compared with model group, the rats' behavior score was lowered obviously（P＜0.05）, the infarct volume was markedly decreased（P＜0.05）,the content of miR-145 was remarkably decreased （P＜0.05）, and the content of SOD was significantly increased（P＜0.05）in the ginsenosides Rb1 group. Compared with ginsenosides Rb1 group, the rats' behavior score was obviously increased （P＜0.05）, the volume of cerebral infarction was markedly increased（P＜0.05）, the content of miR-145 was obviously increased (P＜0.05), and the content of SOD was significantly decreased (P＜0.05) in ginsenosides Rb1+miRNA-145 mimics group. CONCLUSION: Ginsenosides Rb1 can increase the activity of SOD in the brain tissue of rats with cerebral ischemia reperfusion injury by reducing the miRNA-145 content, and protect the brain.

Key words: brain ischemia-reperfusion injury, ginsenoside Rb1, miR-145, superoxide dismutase

CLC Number:

R965.2

XU Hui，ZHANG Minyuan, DAI Qinxue. MiR-145 regulating SOD activity mediated brain protection of ginsenosides Rb1 in rats with cerebral ischemia-reperfusion injury[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2018, 23(11): 1215-1220.

References

［1］王陇德, 王金环, 彭斌,等. 《中国脑卒中防治报告2016》概要［J］. 中国脑血管病杂志, 2017, 14(4)：217-224.
［2］Zhu XY, Ma PS, Wu W, et al. Neuroprotective actions of taurine on hypoxic-ischemic brain damage in neonatal rats［J］. Brain Res Bull, 2016, 124:295-305.
［3］Enzmann G, Kargaran S, Engelhardt B. Ischemia-reperfusion injury in stroke: impact of the brain barriers and brain immune privilege on neutrophil function［J］.Ther Adv Neurol Disord, 2018, 11: 1-15.
［4］Sosa PM, Souza MAD, Mello-Carpes PB. Green tea and red tea from Camellia sinensis partially prevented the motor deficits and striatal oxidative damage induced by hemorrhagic stroke in rats［J］. 2018:5158724.
［5］刘慧琳. 人参皂甙Rb_1对血管性痴呆大鼠认知功能障碍的防治作用［D］. 第四军医大学, 2013.
［6］戴勤学, 张荣, 张民远,等. 腺苷A2a受体介导人参皂苷Rb1增加脑缺血/再灌注损伤大鼠的脑血流量［J］. 中国中西医结合急救杂志, 2016, 23(4):337-340.
［7］Song L, Zhang ZR, Zhang JL, et al. MicroRNA-122 is involved in oxidative stress in isoniazid-induced liver injury in mice［J］. Gene Mol Res Gmr, 2015, 14(4):13258.
［8］Dharap A, Bowen K, Place R, et al. Transient focal ischemia induces extensive temporal changes in rat cerebral microRNAom［J］. J Cereb Blood Flow Metab, 2009, 29(4):675.
［9］郑思道, 于雯, 王宁元. MicroRNA 介导的人参及其活性成分药理作用与机制［J］. 世界中西医结合杂志, 2016, 11(6):880-883.
［10］Longa EZ, Weinstein PR, Carlson S, et al. Reversible middle cerebral artery occlusion without craniectomy in rats［J］. Stroke, 1989, 20(1):84.
［11］Xu W, Gao L, Zheng J, et al. The roles of MicroRNAs in stroke: possible therapeutic targets ［J］. Cell Trans, 2018:963689718773361.
［12］Sohel MH. Extracellular/circulating microRNAs: release mechanisms, functions and challenges［J］. Achi Life Sci, 2016, 10(2):175-186.
［13］Li P, Shen M, Gao F, et al. An antagomir to MicroRNA-106b-5p ameliorates cerebral ischemia and reperfusion injury in rats via inhibiting apoptosis and oxidative stress［J］. Mol Neurobiol, 2016, 54(4):1-21.
［14］Megiorni F, Cialfi S, Cimino G, et al. Elevated levels of miR-145 correlate with SMAD3 down-regulation in cystic fibrosis patients［J］. J Cystic Fibrosis, 2013, 12(6):797-802.
［15］Kandhare AD, Alam J, Patil MVK, et al. Wound healing potential of naringin ointment formulation via regulating the expression of inflammatory, apoptotic and growth mediators in experimental rats［J］. Pharm Biol, 2015, 54(3):419-432.
［16］He JT, Mang J, Mei CL, et al. Neuroprotective effects of exogenous activin A on oxygen-glucose deprivation in PC12 cells［J］. Molecules, 2012, 17(1):315-327.
［17］Zhu XY, Ma PS, Wu W, et al. Neuroprotective actions of taurine on hypoxic-ischemic brain damage in neonatal rats［J］. Brain Res Bull, 2016, 124:295-305.
［18］Kikuchi K, Kawahara K, Tancharoen S, et al. The free radical scavenger edaravone rescues rats from cerebral infarction by attenuating the release of high-mobility group box-1 in neuronal cells［J］. J Pharmacol Expl Ther, 2009, 329(3):865.
［19］李朋. 人参皂甙Rb1对大鼠心肌梗死后氧化应激及心室重构的影响［D］. 华中科技大学, 2006.
［20］Kwok HH, Yue PY, Mak NK, et al. Ginsenoside Rb1 induces type I collagen expression through peroxisome proliferator-activated receptor-delta ［J］. Biochem Pharmacol, 2012, 84(4):532-539.
［21］Chan LS, Yue PY, Kok TW, et al. Ginsenoside-Rb1 promotes adipogenesis through regulation of PPARγ and microRNA-27b［J］. Hormones et métabolisme, 2012, 44(11):819.
［22］Yang Q, Wang X, Cui J, et al. Bidirectional regulation of angiogenesis and miR-18a expression by PNS in the mouse model of tumor complicated by myocardial ischemia［J］. BMC Complement Altern Med,14:183.

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