Study on GM-CSF expression in mouse brain tissue induced by agaricus blazei polysaccharide

Abstract

Abstract: AIM: To identify the molecular mechanisms of GM-CSF expression in mouse brain tissue after stimulation with different concentration of Agaricus Blazei polysaccharide(ABPS).METHODS: The tissue culture technique, GM-CFU clone forming test, and RT-PCR were used to detect GM-CSF expression in mouse brain tissue which treated by ABPS.RESULTS: GM-CSF expression induced by ABPS obviously increased in mouse brain tissue.The appropriate concentration was 4 g·L^-1.The maximum value showed 960 base pairs analyzed byLightFrame Software.CONCLUSION: ABPS can promotethe expression of GM-CSF and facilitate CFU-GMexpression in mouse brain tissue.

Key words: Agaricus Blazei polysaccharide( ABPS), brain tissue, GM-CSF, RT-PCR, expression

CLC Number:

R963

SUN Yan-Hui, HOU Xiang-Lin, LI Xing-Yu. Study on GM-CSF expression in mouse brain tissue induced by agaricus blazei polysaccharide[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(7): 799-802.

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