Effects ant mechanism of silibinin on antagonizing alcoholic fatty liver in rats

Abstract

Abstract: AIM: To investigate the effects and mechanism of silibinin(Sil)on rats with alcoholic fatty liver.METHODS: The animal models with alcoholic fatty liver were prepared in rats by feeding on high caloric food and drinking 10 % alcohol qd for 10 weeks ;after 6-week treatment, the effects of Sil (intragastrointestinally treated 100 mg·kg^-1, qd for 4 weeks)were observed by serum activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (AKP), and serum contents of total cholesterols (TC), triacylglycerol (TG), low-density lipoprotein cholesterol (LDLC), high-density lipoprotein cholesterol (HDL-C), tumor necrosis factor (TNF)-αand transforming growth factor (TGF)-β1, and the contents of TG, malondialdehyde (MDA), glutathione (GSH), and activity of superoxide dismutase (SOD)in the hepatic tissue.Hepatic histopathology was observed by microscope.RESULTS: Serum levels of ALT, AST and AKP decreased in administration of Sil (P<0.05 or 0.01), the levels of TG, LDL-C, TNF-αand TGF-β1 reduced (P<0.05 or 0.01), the concentrations of TG and MDA in the hepatic tissue lowered (P<0.01), and the activity of SOD potentiated (P<0.01).Hydropic and or fatty degeneration of hepatocytes were improved (P<0.01).CONCLUSION: Silibinin can preclude alcoholic fatty liver in rats from drinking alcohol and feeding high caloric food, and pharmacological mechanisms were involved in anti-oxidation and removing free radical, inhibiting lipid peroxidation, adapting blood lipid component, reducing fatty sediment in liver, anti-inflammation and anti-hyperplasia.

Key words: silibinin, ethanolic fatty liver, free radical injure, tumor necrosis factor-α, transforming growth factor-β₁

CLC Number:

TAN Hai-Rong, WU Qian, PAN Jing-Qiang, XIAO Liu-Ying, HAN Chao, LIN Jie-Ru, ZHENG Lin-Ying, LI Bo-Ping. Effects ant mechanism of silibinin on antagonizing alcoholic fatty liver in rats[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2004, 9(7): 803-806.

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