Effects of ketamine on neuroprotection and phospho-p38 protein activation induced by oxygen-glucose deprivation in rat hippocampal slices

Abstract

Abstract: AIM: To investigate the effects of NMDA receptor antagonist ketamine on neuroprotection and the activation of phospho-p38 protein induced by oxygen-glucose deprivation (OGD) in the rat hippocampal slices. METHODS: The OGD model of rat hippocampal slice in vitro was used for the research.The neuroprotective effects of ketamine on the OGD injury of rat hippocampal slices were assessed by 2, 3, 5-triphenyl-tetrazolium chloride (TTC) staining.The changes of phospho-p38 protein level in the hippocampus during ischemia-reperfusion and the effects of ketamine on the phospho-p38 protein activation were examined by Western blot.RESULTS: Ketamine significantly inhibited the decrease of OD in brain slices by TTC staining.The activation of phospho-p38 protein increased after reperfusion 15 min, and it consistently expressed at 2 h.Ketamine (5 and 10 mol·L^-1) markedly inhibited the activation of phospho-p38 protein (P <0.05).CONCLUSION: Ketamine shows the neuroprotective effect related to the inhibition of activation of phospho-p38 protein induced by oxygen-glucose deprivation(OGD) in the rat hippocampal slices.

Key words: brain slice, ischemia-reperfusion, ketamine, p38, Western blot

CLC Number:

R971.3

WU Xing-jun, CHEN Hong-zhuan, LU Yang. Effects of ketamine on neuroprotection and phospho-p38 protein activation induced by oxygen-glucose deprivation in rat hippocampal slices[J]. Chinese Journal of Clinical Pharmacology and Therapeutics, 2005, 10(1): 15-19.

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