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Chinese Journal of Clinical Pharmacology and Therapeutics ›› 2005, Vol. 10 ›› Issue (10): 1108-1111.

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Sequence analysis of muscarinic receptors M2, and M4 expression by cultured rat astrocyte cells

ZHANG Xiao-juan, WANG Hai   

  1. Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100580, China
  • Received:2005-08-29 Revised:2005-09-26 Published:2020-11-23

Abstract: AIM:To clone the muscarinic receptor M2, M4 sequence of astrocyte cell, and compare the geneand protein sequence with those of neuron.METHODS: The specific primers were designed to clone the M2 and M4, sequence of astrocyte cellng to the sequences that of neuron by RT-PCR, and then the sequences were sequenced. RESHLTS AND CONCLUSIONS: The M2 and M4 sequences expression were compared by astrocyte cells and neurons, and the difference was marked. The differences of the gene and protein sequences are evidentof M2 and M4 between astrocyte cell and neuron.

Key words: non-neuronal cholinergic system: astro-cyte cell, gene clone, PCR, sequence analysis, muscarunic receptor

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