Abstract: AIM: To evaluate the affinity of the man-nose receptorof peritoneal macrophage and pulmonary macrophage and theirligands.METHODS: Two kinds of macrophage were acquired through Irrigation of peritoneal cavity and bronchoalveolarlavage(BAL).Afterpurifica-tion and identification,macrophage were incubated with fluorescent isothiocyanate(FITC)-labeled mannosylated albumin(M-FITC-BSA,Sigma A7790)for20 minites,the affinity of Mrand its ligands are measured by flow cytometry(FCM)and fluorescene microscope.RESULTS: There existed Mrin macrophage.The binding of Mrand its ligandswas calcium dependent,which was inhibited by the existence of D-mannose and EDTA,but not by D-galactose.The affinity of peritoneal macrophage Mrand its ligands were greaterthan that of the pulmo-nary macrophage Mrand its ligands.CONCLUSION: The affinity of peritoneal macrophage Mrand its ligands are greaterthan that of the pulmonary macrophage Mrand its ligands.

Key words: macrophages, mannose receptor, li-gands, polysaccharides, drug targeting